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Molecular marker tightly interlocked with muskmelon yellow and green leaf color gene ygl

A molecular marker, yellow-green leaf technology, applied in the field of muskmelon genetic engineering, can solve the problems of unrealized leaf color control gene cloning, unrevealed melon leaf color molecular mechanism, etc.

Active Publication Date: 2018-04-03
HENAN AGRICULTURAL UNIVERSITY
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Problems solved by technology

However, this research work has not yet achieved the cloning of the gene controlling leaf color, let alone revealed the molecular mechanism of the change of melon leaf color

Method used

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  • Molecular marker tightly interlocked with muskmelon yellow and green leaf color gene ygl
  • Molecular marker tightly interlocked with muskmelon yellow and green leaf color gene ygl
  • Molecular marker tightly interlocked with muskmelon yellow and green leaf color gene ygl

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Embodiment

[0040] This embodiment mainly introduces the ygl The fine mapping process of genes, including the construction of genetic segregation populations, preliminary positioning, fine positioning and other processes (analysis process such as image 3 shown), the process involves the development, design and verification of the final molecular markers Indel24 and Indel43, and the relevant experimental procedures are briefly introduced as follows.

[0041] 1. Construction of genetic segregation populations

[0042] The yellow-green melon material NSL73046 was used as the female parent, and the normal material DHL92 of the melon was used as the male parent (it should be explained that during the experiment, considering the availability of materials and convenient operation, the inventor used DHL92 as the male parent. For homozygous normal leaf color materials, relevant experiments can also be carried out), using these two parents to configure a hybrid combination, the results show that ...

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Abstract

The invention relates to a molecular marker in the technical field of muskmelon genetic engineering, and in particular relates to a pair of molecular markers which are used for positioning a muskmelonyellow and green leaf color gene ygl, and are tightly interlocked with the muskmelon yellow and green leaf color gene ygl. When the molecular marker Indel24 is used for positioning the yellow and green leaf color gene ygl, PCR (Polymerase Chain Reaction) amplification can be carried to obtain a characteristic strip which is tightly interlocked with 191 bp; when the molecular marker Indel43 is used for positioning the yellow and green leaf color gene ygl, PCR amplification can be carried to obtain a characteristic strip which is tightly interlocked with 200 bp. The yellow and green leaf colorgene ygl is located between the two molecular markers, and a physical distance is only about 26Kb. By utilizing the result, a physical map between the two molecular markers can be obtained, so that afoundation is laid for final cloning of the gene ygl and establishment of a molecular marker assisted breeding system; meanwhile, a foundation is also laid for development of muskmelon chloroplasts and researches of a chlorophyll synthesis regulation and control network.

Description

technical field [0001] The invention relates to molecular markers in the technical field of melon genetic engineering, in particular to a pair of genes for locating the yellow-green leaf color of melon ygl , and melon yellow-green leaf color gene ygl Tightly linked molecular markers. Background technique [0002] The occurrence of leaf color mutation is mainly due to the direct or indirect interference of the mutated gene with the synthesis and degradation of chlorophyll, which changes the content and proportion of various pigments in the leaves through various channels, and finally causes the leaf color variation. The synthesis and degradation of chlorophyll involve a complex process of coordinated regulation of nuclear genes and chloroplast genes. Mutations in any of the related genes may result in blocked chlorophyll synthesis or defects in chloroplast development, resulting in blocked photosynthesis. The biosynthesis of chlorophyll and the development of chloroplasts i...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 杨路明朱华玉宋芃垚胡建斌孙守如
Owner HENAN AGRICULTURAL UNIVERSITY
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