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Compound for regulation and control of plant chlorophyll synthesis and use thereof

A complex, chlorophyll technology, applied in the field of plant genetic engineering, can solve problems such as complex regulation mechanism of chlorophyll synthesis

Inactive Publication Date: 2016-07-27
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Studies have shown that the regulation mechanism of chlorophyll synthesis is very complex

Method used

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  • Compound for regulation and control of plant chlorophyll synthesis and use thereof
  • Compound for regulation and control of plant chlorophyll synthesis and use thereof
  • Compound for regulation and control of plant chlorophyll synthesis and use thereof

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Embodiment Construction

[0064] After extensive and in-depth research, the inventors unexpectedly discovered that there is a SCL27:PORC complex in plants. Using this complex, the present inventors used the overexpression or silent regulation of the effective PORC gene to regulate plant chlorophyll content, leaf color and other traits for the first time, so as to achieve the purpose of controlling night color or regulating plant photosynthesis efficiency. The present invention has been completed on this basis.

[0065] As used herein, the terms "complex of the present invention", "SCL27-PORC complex", "protein-nucleic acid complex of the present invention", or "SCL27 protein-PORC gene complex" are used interchangeably and all refer to SCL27 The protein (or its active fragment) binds to certain specific regions of the promoter region of the PORC gene to form a complex. The research of the present invention shows that the compound plays a central role in regulating chlorophyll synthesis.

[0066] PORC gene ...

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Abstract

The invention provides a compound for regulation and control of plant chlorophyll synthesis and a use thereof. The invention provides a protein-nucleic acid compound. A SCL27 protein is bonded to a specific area of a PORC gene so that the compound is formed. An experiment proves that the compound can regulate and control PORC gene expression, simply and effectively regulate and control plant chlorophyll synthesis and adjust plant chlorophyll content and blade color thereby controlling a leaf color or regulating plant photosynthesis efficiency.

Description

Technical field [0001] The present invention relates to the field of plant genetic engineering. Specifically, the present invention provides a compound, nucleic acid sequence and application thereof for regulating the synthesis of plant chlorophyll. Background technique [0002] The photoreaction of photosynthesis in higher green plants must be completed in the chloroplast. The main molecules in the chloroplast that receive light energy are chlorophyll, including chlorophyll a and chlorophyll b. Chlorophyll can capture photons and convert light energy into chemical energy, which is the synthesis of plants Metabolism and growth provide the driving force. [0003] Chlorophyll is encoded by chloroplast DNA, but its expression and location are strictly controlled by nuclear genes. Since chlorophyll is the basis of plant photosynthesis, research and development of related genes and control functions involved in this pathway play an important role in improving the photosynthetic efficie...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/113G01N33/53
Inventor 黄继荣麻兆雪
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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