The invention discloses a preparation method of
lactic acid, which takes lignocellulose as
raw material, and is low in cost and high in efficiency, wherein the preparation method specifically comprises the following steps of: separating from lignocellulose to store
pediococcus acidilactici, carrying out
liquid culture on the
pediococcus acidilactici to obtain
cell bacteria taken as seeds, inoculating into a reactor with high lignocellulose content, adding
cellulase, and synchronously carrying out saccharification and high-temperature
fermentation to generate the
lactic acid; or firstly saccharifying the
cellulase of the lignocellulose to obtain
sugar-containing
hydrolysate, then carrying out high-temperature
lactic acid fermentation by the
hydrolysate to obtain the lactic acid; or uniformly blending the
pediococcus acidilactici and the lignocellulose
raw material, carrying out
solid state cultivation, adding the
pediococcus acidilactici-containing lignocellulose
raw material into a
fermentation tank, adding the
cellulase, and synchronously carrying out saccharification and high-temperature fermentation to prepare the lactic acid. By adopting unique fermentation technology and
microorganism, the preparation method is high in product concentration, simple in process, and low in cost; and particularly by taking the lignocellulose as raw material, the preparation method is wide in production space and development prospect.