Method for preparing squalene by adopting lignocellulose

Abstract

The invention provides a process for preparing squalene by taking lignocellulosic agricultural and forestry wastes as raw materials, which aims at problems in the aspect of the production of squaleneby microbial fermentation in the prior art. The process comprises the steps of pre-treatment, saccharification, yeast-like fungi fermentation and squalene extraction and the like. According to the process provided by the invention, lignocellulose biological saccharification is combined with yeast-like fungi high-density fermentation; by adopting lignocellulose biomass as raw material, on the one hand, the cost of enzyme usage and the production cost of squalene microbial fermentation in a saccharification stage are significantly reduced; on the other hand, the problem of comprehensive utilization of the agricultural and forestry wastes is solved. In addition, medium and fermentation medium in the lignocellulose saccharification stage can be recycled, water can be saved and usage of chemicals can be reduced, and has significant effect on reducing the discharge of wastewater and reducing the cost. In the process provided by the invention, the squalene yield can reach 13 g / L, accounting for 5% of the dry weight of thallus, and the purity of refined squalene is higher than 93%.

Description

technical field [0001] The invention relates to the field of microbial fermentation engineering, in particular to a method for producing squalene by using lignocellulose raw materials. Background technique [0002] Squalene is a non-toxic marine biologically active substance with the effect of preventing and curing diseases. Its chemical name is 2,6,10,15,19,23-hexamethyl-2,6,10,14 ,18,22-Tetracosahexaene has many physiological functions such as increasing the activity of superoxide dismutase in the body, enhancing the body's immunity, improving sexual function, anti-aging, anti-fatigue, anti-tumor, etc. It is widely used in medicine, Beauty, cosmetics, food and other fields. [0003] Currently, the main source of squalene is fish oil, especially shark liver oil. However, with the depletion of fishery resources and the improvement of relevant regulations, people are trying to find other sources of squalene; such as from olive oil, palm oil and other oil crops, or from amar...

AI Technical Summary

Problems solved by technology

However, since the activity and stability of exogenously added proteins will decrease with the progress of the saccharification process, the consumption of enzymes requires an increase in the amount or frequency of enzyme addition

In addition, these non-cellulosomal proteins are added to the saccharification system as free enzymes, which cannot interact with cellulosomes, and will significantly reduce the synergistic effect with other enzymes in the system, inevitably resulting in additional enzymes that are greater than the demand, resulting in The high cost of enzyme preparations

Not only that, this saccharification strategy that relies on the addition of exogenous free enzymes has complex processes, high equipment requirements, and conversion efficiency that cannot meet the requirements of industrial production.

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