Method for producing sophorolipid through fermentation of lignocellulose material

A technology for lignocellulose and microbial fermentation biosurfactant, which is applied in the field of using lignocellulosic material to ferment and produce microbial fermentation biosurfactant by using a Wick yeast variant, and achieves the effects of improving energy shortage, simple process and easy separation.

Inactive Publication Date: 2012-06-13
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the fact that there is no report on the production of sophorolipids by fermentation of biomass resources at present, the problem to be solved in the present invention is to provide a method for the production of sophorolipids

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  • Method for producing sophorolipid through fermentation of lignocellulose material
  • Method for producing sophorolipid through fermentation of lignocellulose material
  • Method for producing sophorolipid through fermentation of lignocellulose material

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Embodiment 1

[0027] (1) Preparation of cellulase solution: Penicillium decumbens JU-A10 was inoculated into the enzyme-producing medium in a conventional amount, cultured at 30°C, shaking at 250 rpm for 96 hours to produce enzyme, centrifuged at 6000 rpm for 30 minutes, and collected The supernatant is the crude cellulase enzyme solution;

[0028] The above-mentioned enzyme production medium formula is: 20g / l xylose residue, 30g / l bran, 6g / l microcrystalline cellulose, 5g / l soybean meal, 2g / l ammonium sulfate, 1g / l urea, 2g / l nitric acid Sodium, 3g / l potassium dihydrogen phosphate, 0.5g / l magnesium sulfate, 3ml / l Tween 80;

[0029] (2) Preparation of saccharification solution: take the delignified xylose residue, add cellulase solution to 25 FPA per gram of dry substrate, and saccharify at 45°C for 72 hours; collect the saccharification solution in a water bath at 80°C for 30 minutes to make residual cellulose The enzyme is inactivated, and the saccharification solution is centrifuged at 8000 ...

Embodiment 2

[0036] (1) Preparation of cellulase solution: Inoculate Penicillium decumbens in the enzyme-producing medium in a conventional amount, culture at 30°C, shaking at 250 rpm for 84 hours to produce enzyme, centrifuge at 6000 rpm for 30 minutes, and collect the supernatant to obtain cellulose. Enzyme crude enzyme solution;

[0037] The above-mentioned enzyme production medium formula is: 20g / l xylose residue, 30g / l bran, 6g / l microcrystalline cellulose, 5g / l soybean meal, 2g / l ammonium sulfate, 1g / l urea, 2g / l nitric acid Sodium, 3g / l potassium dihydrogen phosphate, 0.5g / l magnesium sulfate, 3ml / l Tween 80;

[0038] (2) Preparation of saccharification solution: take the delignified xylose residue, add cellulase solution to 30 FPA per gram of dry substrate, and saccharify at 45°C for 72 hours; collect the saccharification solution in a water bath at 80°C for 30 minutes to make residual cellulose The enzyme is inactivated, and then the saccharification solution is centrifuged at 9000 rp...

Embodiment 3

[0045] (1) Preparation of saccharification solution: take the delignified xylose residue, add 25 FPA per gram of dry substrate to a commercial cellulase solution (from Qingdao Kang Dien Biotechnology Co., Ltd.), the buffer system is pH 4.8 Saccharification with 0.05M HAC-NaAc buffer solution at 45℃ for 72h; Collect the saccharification solution in a water bath at 80℃ for 30min to inactivate the residual cellulase, and then centrifuge the saccharification solution at 8000rpm for 10min to obtain a clear saccharification solution;

[0046] (2) Detoxification treatment of the saccharification solution: heat the saccharification solution to 80°C in a water bath, add 1.0% by mass activated carbon, stir for 30 minutes, and then remove the activated carbon by vacuum filtration to obtain a detoxified saccharification solution;

[0047] (3) Use saccharification solution to prepare single-cell oil: prepare an oil-producing saccharification solution fermentation medium with a formula of detoxif...

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Abstract

The invention discloses a method for producing sophorolipid through fermentation of a lignocellulose material. The method comprises the steps of: carrying out shaking table oscillation fermentation on hydrolysate of delignified xylose residue, xylose residue, corn straw, corn cob powder, grass meal and other biomass resources and single cell oil generated by the hydrolysate in the presence of Wickerhamiella domercqiae variant at 30+/-2 DEG C and 150-300 rpm to obtain fermentation liquid containing sophorolipid, and carrying out rough extraction on the fermentation liquid to obtain sophorolipid. According to the method disclosed by the invention, the lignocellulose saccharification liquid is used to replace the sugar required for producing the single cell oil and sophorolipid, and the single cell oil is used to replace a hydrophobic substrate required for producing sophorolipid, thus obtained sophorolipid is easy to separate and high in yield; the method has the characteristics of richand inexpensive raw materials, simple and convenient process and low cost; a great deal of biomass resources are utilized, and agricultural wastes are treated; and the method has important practical significance and important economic value in improving energy insufficiency condition in China, guaranteeing sustainable energy supply in China and alleviating energy crisis.

Description

Technical field [0001] The invention relates to a method for producing sophorolipid by fermentation, in particular to a method for producing sophorolipid by fermenting Wickerhamiella domercqiae var.sophorolipid with lignocellulose material. Background technique [0002] Sophorolipid is an important biosurfactant, mainly obtained through microbial fermentation. Generally, when a hydrophobic carbon source and a hydrophilic carbon source exist simultaneously, sophorolipid-producing bacteria can secrete a large amount of sophorolipid. Compared with chemically synthesized surfactants, sophorolipids have excellent surface properties, stable emulsification ability, and obvious foaming effect, but also have good biodegradability, biocompatibility, etc., and have antibacterial and anti-tumor properties. , Anti-virus and other biological activities. These advantages make Sophorolipids have great application prospects in business. [0003] As a biosurfactant, sophorolipid can be used in va...

Claims

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Application Information

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IPC IPC(8): C12P19/12C12R1/645C12R1/845C12R1/80
Inventor 宋欣马晓静
Owner SHANDONG UNIV
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