37 results about "Capsaloides" patented technology

The present invention relates to the production of capsaicinoid compounds including Capsaicin and Nonivamide via microbial fermentation.

The invention discloses a method for preparing a capsanthin microcapsule, comprising the following steps of: (1) preparing capsanthin core phase; (2) preparing modified starch capsule phase; (3) mixing the capsanthin core phase and the modified starch capsule phase and emulsifying and homogenizing the mixture into emulsified liquid with a nanometer grain diameter at high pressure; and (4) drying the emulsified liquid to obtain the capsanthin microcapsule. The method for preparing the capsanthin microcapsule microencapsulates capsanthin by adopting microencapsulation technology and coats the capsanthin (core) into the microcapsule with a nanometer grain diameter by using single capsule modified starch. The method for preparing the capsanthin microcapsule has simple process, and the prepared product has good water solubility and high stability.

The present invention relates to a compound preparation for treating large freshwater fish melon worm disease and its application. The components and mass percentage ranges of the compound preparation are: 8-12 wt% of hemosanone hydrobromide, 32 wt% of Guanzhong extract ~35wt%, capsaicin 1~2wt%, dithiocyanomethane 0.5~1.5wt%, and the rest are dissolving agents, the sum of the above raw materials is 100%. The combination of hemosanone hydrobromide, the ethanol extract of Guanzhong and capsaicin has obvious synergies, and has good water solubility and stable drug effect. The secondary infection of mold and bacteria can significantly reduce the mortality rate of large freshwater fish melonosis, and improve the economic benefits of breeding.

The invention relates to a method for efficiently separating and purifying bacterial strains of Phytophthora capsici from a diseased tissue of aging Phytophthora capsici Leonian, the method includes steps of acquisition of the diseased tissue of Phytophthora capsici Leonian, surface sterilization, preparation of Cucumis sativus, surface sterilization of the Cucumis sativus, culturing the diseasedtissue and the Cucumis sativus, making a selective culture medium, and separation, purification and storage of the Phytophthora capsici. The method ensures separating Phytophthora capsici from the aging disease tissue, and the method is simple, easy, efficient, free from Miscellaneous bacteria pollution and adapted to separation of mass bacterial strains.

The invention relates to a single spore isolation method of phytophthora capsici zoospores. The single spore isolation method comprises the steps that phytophthora capsici is inoculated to a V8 culture medium, sealing is performed with a sealing membrane, culture at the constant temperature of 25 DEG C is performed for 5 days, then the sealing membrane is removed, irradiation is performed for 24 hours, sterile water of 4 DEG C is added to perform induction and constant-temperature release respectively for 25 minutes so as to obtain a zoospore suspension, the suspension is diluted to reach the standard that 1-2 spores exist in each (10*) view, 1 mL of the suspension is taken and added to a V8 culture medium flat plate containing antibiotics in an evenly coated mode, constant-temperature standing is performed for 6 hours, a single germinated zoospore is found through an inverted microscope and is cut by using a scalpel to form a 0.5 cm square mark, it is confirmed that single spores are transferred to a flat antibiotic plate, and purified spores are single spore plants. The single spore isolation method is simple and convenient to operate, high in accuracy and good in rapid separating effect, adopts simple devices and can separate single spores of a large number of phytophthora capsici zoospores within a short time.

The invention discloses a Phytophthora capsici LAMP primer and a rapid detection method thereof, which are specially used for the specific detection of Phytophthora capsici. Mainly designed a LAMP primer (F3, B3, FIP, BIP) of Phytophthora capsici, using this primer for constant temperature amplification and adding 50μM Calcein-500μM MnCl2 color reagent or agarose gel electrophoresis detection, it can be observed to green fluorescence or a trapezoidal band characteristic of LAMP. The present invention can be used for rapid, sensitive and accurate detection of Phytophthora capsici in plants and soil infected by Phytophthora capsici in production practice, and can be used for early diagnosis of field diseases and monitoring and identification of pathogens. Provide reliable technical and theoretical basis for disease control.

The invention provides a PCR (Polymerase Chain Reaction) detection primer for phytophthora capsici leonian. The primer comprises Enl4s and Enl1a (as shown in Seq ID No.1 and 2). Four pairs of specific primers capable of quickly detecting phytophthora capsici leonian germs can be obtained based on the sequence difference between the Enl and Ypt genes of the phytophthora capsici leonian and other phytophthora nicotianaes: Enl2s / Enl3a, Enl4s / Enl1a, Ypt2s / Ypt3a and Ypt2s / Ypt5a, wherein the sensitivity of Enl4s / Enl1a is the highest. A PCR detection system is established on the basis, which is capable of quickly and accurately detecting the phytophthora capsici leonian from complex pathogenic bacteria environments in the tissues of plants having diseases and soil. A detection kit established according to the method is simple and convenient for operation, good in specificity and high in sensitivity; and the detection kit is capable of detecting the propagules in various forms of the phytophthora capsici leonian, such as hypha, oospore, zoospore and the like, thereby having great significance in the aspects of early warning on the epidemic situation of the phytophthora capsici leonian, pathogen supervision on the epidemic area and the like.