36 results about "Capsaloides" patented technology

The invention provides a pectin methylesterase gene Pcpme l cloned from phytophthora capsici and a preparation technique of a protein thereof, belonging to the technical field of biology. The invention proves that the pectin methylesterase gene Pcpme l effectively participates in the processes of infecting a host by the phytophthora capsici and causing the disease course of phytophthora capsici leonian on the basis of gene and protein levels and also proves that the protein coded by the pectin methylesterase gene Pcpme l has property of destroying leaf tissue cells and cell wall structures thereof to enable destroyed parts to generate obvious symptoms on the basis of a cell chemistry technique, thus the pectin methylesterase gene Pcpme l is an important target pathogenic gene of a coded phytophthora capsici pectin methylesterase gene cluster. The invention provides sufficient technical reserve for further researching a germ molecule detection technique.

The invention relates to the technical field of external medicines for hypertrophic scars, and particularly relates to a preparation method and an application of capsaicin-collagen sponge. The preparation method comprises the following steps: 1 preparing collagen powder from tendo calcaneus; 2 extracting the collagen powder with pepsin, so as to obtain an extract liquid; 3 centrifugally collecting supernate; 4 separating out collagen by adding sodium hydroxide, and washing, so as to prepare high-purity collagen; 5 redissolving the collagen with ethylic acid; and 6 evenly mixing the collagen liquid, glycerinum and capsaicin, and then carrying out freeze-drying, so as to obtain the capsaicin-collagen sponge. The capsaicin-collagen sponge prepared by the method is applied to tissue trauma repair and inhibition of the hypertrophic scars in the tissue trauma repair process, and has the advantage of good percutaneous permeability; the drug liver first-pass effect can be avoided; and the thrill to the skin can be reduced.

The invention provides a method for rapid separation of phytophthora capsici and a special culture medium thereof, and belongs to the technical field of phytopathology research. The separation method comprises the steps of sick sample treatment, disinfection, culture medium preparation, and the like. The sick sample is selected from a freshly-picked sick stem, and a sick sample tissue of 9-11 cm is taken with a juncture of the sick part and the healthy part of the sick sample as a center; the tissue is cleaned with tap water, and is dried in the air to remove surface moisture; the dried tissue is uniformly sprayed with 75% alcohol, then soaked with a 1% sodium hypochlorite solution, and cleaned with sterile water; after drying in the air, the disinfected large block of the sick sample is cut into small blocks on an aseptic operating floor; and separation culture is performed with special culture medium. With the method of the invention, the separation rate of phytophthora capsici reaches 81.99% on average, which is increased by 32.6% when compared with the separation rate of a routine (small block disinfection treatment, small block separation) treatment method on average; the separation time is only 31.6 minutes on average; the separation time is shortened by 50.1% on average; and the operations are simple and practical.

The invention relates to the technical field of microorganisms, the artemisia japonica endophytic fungi is isolated from artemisia japonica plant living body which is collected from suburb of Guiyang City Guizhou Province by endophytic fungi separation technology, by microbial taxonomic identification, the artemisia japonica endophytic fungi is named as pestalotiopsis uvicola GMH31, the strain is preserved in China Center For Type Culture Collection (CCTCC), the preservation date is June 30, 2014, and the preservation registration number is CCTCC NO:M2014303. The most striking feature of the Pestalotiopsis uvicola GMH31 is that the fermentation liquor of the strain can produce active metabolites with inhibiting effects on kiwi fruit sclerotinia sclerotiorum, phytophthora capsici and other pathogenic fungi of plant fungal diseases, and the Pestalotiopsis uvicola is an important biological resource in agriculture.

The invention discloses key proteins Pcsec4-1 and Pcsec4-2 for regulating and controlling secretion of extracellular vesicles from phytophthora capsici as well as a coding gene and application thereof. The key protein sequence for regulating and controlling vesicle secretion provided by the invention is shown as a sequence 2 and a sequence 4; the coding gene is shown as a sequence 1 and a sequence 3. Experiments prove that the protein provided by the invention plays an important role in the growth and development process of Phytophthora capsici, specifically, after the protein is deleted, the growth of phytophthora capsici hyphae is slowed down, the number of zoospores is reduced, the pathogenicity is reduced, the secretion amount of extracellular vesicles is reduced, and the like. The conclusions provide a technical basis for exploring phytophthora capsici development and a pathogenic molecular mechanism, and provide a potential molecular target for research and development of novel bactericides in the future.

The invention provides a taste-masking capsaicin particle and a preparation method thereof, the taste-masking capsaicin particle comprises a particle material and a coating layer, the particle material is coated with the coating layer, and the particle material is composed of the following components in percentage by weight: 1-30% of capsaicin, 70-95% of an auxiliary material and 0.5-3% of an adhesive. The capsaicin raw material is granulated, the particle state of the capsaicin raw material is improved, so that the capsaicin raw material is easy to coat, the oral irritation of capsaicin is relieved to a certain extent, the capsaicin particles are coated, and the surfaces of the capsaicin particles are coated with one or more coating layers, so that the capsaicin is prevented from being in direct contact with a human body; therefore, an excellent taste masking effect is achieved, and the oral irritation of capsaicin is greatly reduced.

The invention discloses a plant expression vector capable of effectively interfering a phytophthora capsici cellulose synthase 3 gene through host-induced gene silencing and application thereof. The plant expression vector disclosed by the invention can be transcribed to form a double-stranded RNA with a reverse complementary structure, and the double-stranded RNA is formed by respectively inserting a nucleotide sequence shown as SEQ ID No.1 or SEQ ID No.2 into the vector twice in the forward direction and the reverse direction. The invention also discloses application of the expression vectors in preventing and treating plant diseases caused by phytophthora capsici. The plant expressing the vector provided by the invention has obviously enhanced disease resistance to phytophthora capsici, the expression of the cellulose synthase 3 gene of the phytophthora capsici infecting the plants is significantly inhibited, and the cellulose synthase 3 gene is necessary for the development and pathopoiesis of the phytophthora capsici, therefore, the plant expression vector disclosed by the invention can effectively inhibit plant infection caused by phytophthora capsici, and an effective way is provided for preventing and treating related diseases caused by phytophthora capsici.