48 results about "Betaglucin" patented technology

The invention relates to the field of genetic engineering, in particular to medium-temperature beta-glucosidase BglA1, gene of the same and application of the same. The novel medium-temperature beta-glucosidase BglA1 contains amino acid sequence shown like SEQ ID NO.1. The invention further provides the gene for encoding the medium-temperature beta-glucosidase BglA1, recombination carrier containing the gene, recombination bacterial strain containing the gene and application of the medium-temperature beta-glucosidase BglA1, wherein nucleotide sequence of the gene is shown like SEQ ID NO.2. The optimum potential of hydrogen (pH) of the medium-temperature beta-glucosidase BglA1 is 6.0 and has higher enzymatic activity when the pH is from 5.0 to 7.0. Optimum temperature of the medium-temperature beta-glucosidase BglA1 is 50 DEG C, thereby being good in heat resistance. Thus, the medium-temperature beta-glucosidase BglA1 can be applied to industrial production with requirements for heat resistance.

The present invention is directed to extracts of cranberries (Vaccinium macrocarpon) comprising either mixed flavonols that are substantially free of anthocyanins and proanthocyanidins or a purified cranberry flavonol compound, including myricetin-3-β-xylopyranoside, quercetin-3-β-glucoside, quercetin-3-α-arabinopyranoside, 3′-methoxyquercetin-3-α-xylopyranoside, quercetin-3-O-(6″-p-coumaroyl)-β-galactoside, and quercetin-3-O-(6″-benzoyl)-β-galactoside. The present invention also embodies the use of those extracts, as well as extracts comprising the cranberry flavonol compound quercetin-3-α-arabinofuranoside, for the treatment of inflammatory disorders. Pharmaceutical, food, dietary supplement, and cosmetic compositions utilizing the extracts or compounds of the present invention are also recited.

The invention relates to the fields of gene engineering and genetic engineering, and in particular relates to beta-glucosaccharase mutant M36E with high catalytic efficiency as well as a coding gene and applications of the beta-glucosaccharase mutant M36E. For the mutant M36E of beta-glucosaccharase with heat resistance, high-temperature acid beta-glucosaccharase BGL3A derived from Talaromyces leycettanus JCM12802 is taken as a female parent, site-specific mutagenesis is carried out on the sequence of the beta-glucosaccharase by adopting a molecular biological technology, and the amino acid sequence is shown in SEQ ID NO.1. Under the modification condition, the affinity of the mutant for cellobiose is improved by 2.1 times compared with that of the wild type (before mutation), the catalytic efficiency is improved by 2.3 times, and the optimum reaction pH and temperature are invariable. With the adoption of the strategy, the catalytic efficiency of beta-glucosaccharase can be greatly improved, and an application foundation is provided for the beta-glucosaccharase in the industrial production fields including food, bioethanol and the like. The strategy has great guiding significances for the improvement of the catalytic efficiencies of beta-glucosaccharase and other enzymes.

The invention relates to beta-glucosidase Mut1b as well as an expressed gene and application thereof, which belong to the technical field of biological engineering. The invention provides gene mut1b for expressing the beta-glucosidase Mut1b, wherein the nucleotide sequence of the gene mut1b is shown as SEQ ID NO.1; and the invention also provides the beta-glucosidase Mut1b, wherein the amino acidsequence of the beta-glucosidase Mut1b is shown as SEQ ID NO.2. Mutant Mut1b with high hydrolyzing activity for the beta-glucosidase can be used for constructing a high-yield cellulase strain and a high-activity cellulase system in an industry, and the efficiency of enzymolysis of cellulose is effectively enhanced; and furthermore, the beta-glucosidase Mut1b is beneficial to the high-efficiency conversion of agricultural waste resources and has important economic value and social benefit.

The invention relates to an active extract composition of rubia cordifolia with exact pharmacologic action. The composition is characterized by comprising 1, 3, 6-trihydroxyl-methyl anthraquinone-3-O-alpha-rhamnose (1->2)-beta-glucoside and mollugin, and the total content of the two active ingredients is greater than 90%. The weight ratio of the 1, 3, 6-trihydroxyl-methyl anthraquinone-3-O-alpha-rhamnose (1-2)-beta-glucoside to the mollugin in the composition is 1:(0.5-5). The invention further relates to an application of the active extract composition of rubia cordifolia in preparing medicines for treating leucopenia.

The invention discloses a pretreatment method for analyzing tetrabromobisphenol A in biologic urine, relates to a detection and analysis technique of organic pollutant in a biologic sample, and provides a pretreatment technique for testing the content of tetrabromobisphenol A in urine. The pretreatment method comprises the following steps: regulating the pH value in a urine sample, performing enzymolysis on the urine in a mode of adding beta-glucuronide / aryl sulfuric acid lipase, enriching enzymatic hydrolysate through an ENVI-18 column, washing the enriched eluant by using a 1% hydrochloric acid solution and dichloromethane in a vortex and shaking manner, and subsequently washing a recycled organic layer by using distilled water for another time. The detection on the sample by using an ultra-high performance liquid chromatography-tandem mass spectrometer shows that the method is simple to operate, high in recycling rate and good in impurity moving effect, is applicable to pretreatment on the biologic urine sample, and is particularly applicable to extraction and purification operation on a large-scale biologic urine sample. By adopting the method, conjugate of the tetrabromobisphenol A in the sample can be effectively decomposed, the urine sample can be effectively purified, and support is provided for detection on the tetrabromobisphenol A in the urine.

The invention discloses a double-antibody sandwich method for detecting beta-glucuronidase in escherichia coli cells of food, belonging to the technical field of immunoassay. The double-antibody sandwich method provided by the invention comprises the following steps of carrying out immune on seven-week BALB / c mice by using escherichia coli beta-glucuronidase (EC3.2.1.31), and fusing and screening to obtain ten monoclonal antibodies, respectively marking HRP and carrying out pairing; and establishing an sandwich ELISA analytical method by utilizing CGMCC No.7209 as a coating antibody, utilizing a CGMCC No.7211 as an enzyme labelled antibody, and utilizing the recombination expressive escherichia coli beta-glucuronidase as a standard substance. According to the double-antibody sandwich method provided by the invention, the beta-glucuronidase in escherichia coli ATCC 25922 detection cells is cracked, the escherichia coli is detected, and the LOD is 3.27*10^4cfu / mL; according to the method, the monoclonal antibody of a landmark of the escherichia coli, namely beta-glucuronidase, is prepared, the double-antibody sandwich method for detecting the escherichia coli is established, the method and salmonella, E.coli, enterobacter sakazakii, staphylococcus aureus and listeria monocytogenes do not have cross reaction, and the new detection method is provided for detecting the escherichia coli in food.

The invention discloses a method for producing ethanol by using lychee dregs as raw materials. The method comprises the following steps of: a, drying and crushing fresh lychee dregs, and screening with a 30-mesh screen; b, immersing the obtained lychee dreg powder with water of which the weight is 3-6 times that of the lychee dreg powder; adjusting the pH value to 4.0-4.8; based on the weight of the lychee dreg powder, adding 30-60U / g of cellulase and 15-20CBU / g of beta-glucosidase; performing enzymolysis at 45-50 DEG C for 3-5 hours; and filtering; c, based on the weight of the filtrate, adding 0.08-0.12% of urea, and sterilizing at 110-120 DEG C for 10-15 minutes; and d, based on the volume of the filtrate, adding 4.5-5.5% of saccharomyces cerevisiae seed liquid; performing sealed fermentation for 70-75 hours in an environment with temperature of 30-36 DEG C and humidity of 75-85%; and distilling and collecting to obtain the ethanol. Through the method, the carbohydrates in the lychee dregs can be sufficiently used, and the yield of the ethanol can reach about 8.1kg of ethanol per 100kg of lychee dregs; the pollution and discharge are reduced through comprehensive utilization of the lychee dregs; the energy consumption is reduced; and the method has a wide prospect.

A composite enzyme product as the additive of forage for aquatic animals to increase the utilization rate of forage and the resistance to diseases, promote growth, and decrease the contents of organic substances, N, P, etc in excreta is proportionally prepared from beta-glucosidase, xylanase, beta-mannase, neutral phytase, amylase, neutral proteinase, acidic proteinase, antioxidant, anti-mildew agent. Its preparing process is also disclosed.

The invention provides a process for producing a β-glycoside compound represented by formula (3), characterized in that the process includes causing to react a cyclic alkene compound represented by formula (1) or (2) with a nucleophile in the presence of a transition metal catalyst.

The invention provides a Beta-glucosaccharase gene S-bgl3 and application of the Beta-glucosaccharase gene S-bgl3. Beta-glucosaccharase gene S-bgl3 has the nucleotide sequence as shown in SEQ ID NO: 1; and the genetically coded Beta-glucosaccharase S-bgl3 has the amino acid sequence as shown in SEQ ID NO: 2. The Beta-glucosaccharase S-bgl3 can be applied to the decomposition of cellobiose.

The invention discloses a novel Gus dyeing liquid preparation method which comprises the following steps: (1) preparing a K3[Fe(CN)6] solution and a K4[Fe(CN)6] solution; (2) preparing a PBS (phosphate buffer solution); and (3) preparing dyeing liquid, namely dissolving 5-bromine-4-chlorine-3-benzpyrole-beta-glucuronide ester in methyl alcohol, and sequentially adding the K3[Fe(CN)6] solution, the K4[Fe(CN)6] solution, methyl alcohol, 10% Triton-100 and the PBS after 5-bromine-4-chlorine-3-benzpyrole-beta-glucuronide ester is completely dissolved. The novel Gus dyeing liquid preparation method is relatively simple in preparation process and easy to operate; all the reagents are non-toxic and harmless reagents and cannot injure a human body or pollute an environment; the prepared dyeing liquid has a good dyeing effect; generally, the dyeing liquid can develop a color after 1 hour compared with other dyeing liquid needing a night, so that the test time is shortened.

The invention discloses a double-antibody sandwich method for detecting beta-glucuronidase in escherichia coli cells of food, belonging to the technical field of immunoassay. The double-antibody sandwich method provided by the invention comprises the following steps of carrying out immune on seven-week BALB / c mice by using escherichia coli beta-glucuronidase (EC3.2.1.31), and fusing and screening to obtain ten monoclonal antibodies, respectively marking HRP and carrying out pairing; and establishing an sandwich ELISA analytical method by utilizing CGMCC No.7209 as a coating antibody, utilizing a CGMCC No.7211 as an enzyme labelled antibody, and utilizing the recombination expressive escherichia coli beta-glucuronidase as a standard substance. According to the double-antibody sandwich method provided by the invention, the beta-glucuronidase in escherichia coli ATCC 25922 detection cells is cracked, the escherichia coli is detected, and the LOD is 3.27*10^4cfu / mL; according to the method, the monoclonal antibody of a landmark of the escherichia coli, namely beta-glucuronidase, is prepared, the double-antibody sandwich method for detecting the escherichia coli is established, the method and salmonella, E.coli, enterobacter sakazakii, staphylococcus aureus and listeria monocytogenes do not have cross reaction, and the new detection method is provided for detecting the escherichia coli in food.

The invention relates to high-sugar-tolerant beta-glucosaccharase Bg14, and an expressed gene and application thereof. The amino acid sequence is shown as SEQ ID NO.1. The gene of the high-sugar-tolerant beta-glucosaccharase Bg14 of aspergillusniger is cloned, recombined and expressed for the first time; and the glucose-tolerant coefficient Ki of the recombinase Bg14 is 2 mol / L.