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Beta-glucosaccharase gene S-bgl3 and application thereof

A glucosidase and gene technology, applied in the β-glucosidase gene S-bgl3 and its application fields, can solve the problems of limited application range, low cellulase activity, and affecting enzymatic hydrolysis efficiency

Inactive Publication Date: 2013-05-22
GUANGXI ACAD OF SCI
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, there are a variety of mycotoxins in the fermentation products of Trichoderma and the obtained cellulase activity is low, especially the activity of β-glucosidase is very low, resulting in the accumulation of cellobiose in the reaction system and affecting the enzymatic hydrolysis efficiency, so its application The scope is limited (Han Xiao, Chen Jienan, Wang Yiqiang, 2008, Research progress on cloning and expression of β-glucosidase gene

Method used

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  • Beta-glucosaccharase gene S-bgl3 and application thereof
  • Beta-glucosaccharase gene S-bgl3 and application thereof
  • Beta-glucosaccharase gene S-bgl3 and application thereof

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Embodiment Construction

[0020] The following implementation methods are for better explaining the present invention, but should not be construed as limiting the present invention.

[0021] The materials used in the examples of the present invention include: Escherichia coli (Escherichia coli) strain XL1-blue was purchased from Dalian TaKaRa Company; expression vector pSE380 was purchased from Stratagene Company; TaKaRa, MBI Corporation.

[0022] The present invention will be described in detail below by embodiment:

[0023] 1) Extraction of Streptomyces sp.GX6 genomic DNA

[0024] Genomic DNA of Streptomyces sp.GX6 was extracted according to the instruction of BioFlux company's Bacterial Genomic DNA Extraction Kit Biospin Bacteria Genomic DNA Extraction Kit (Cat. No. BSC12S1).

[0025] The extracted genomic DNA of Streptomyces sp.GX6 was sent to Huada Gene Company for genome sequence determination.

[0026] 2) Analysis of the gene sequence encoding β-glucosidase in the genome sequence of Streptomyce...

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Abstract

The invention provides a Beta-glucosaccharase gene S-bgl3 and application of the Beta-glucosaccharase gene S-bgl3. Beta-glucosaccharase gene S-bgl3 has the nucleotide sequence as shown in SEQ ID NO: 1; and the genetically coded Beta-glucosaccharase S-bgl3 has the amino acid sequence as shown in SEQ ID NO: 2. The Beta-glucosaccharase S-bgl3 can be applied to the decomposition of cellobiose.

Description

technical field [0001] The present invention relates to a β-glucosidase gene S-bgl3 and its application. The protein coded by the gene can be used to degrade cellobiose. Background technique [0002] β-glucosidase (beta-glucosidase, EC3.2.1.21), also known as β-D-glucoside glucohydrolase, is a kind of cellulase. β-glucosidase is of great value in carbohydrate metabolism of humans, animals, plants and microorganisms (Ramakrishnan Anish, Mohammad Safikur Rahman, and Mala Rao, 2007, Application of cellulase from an alkalothermophilic Thermomonospora sp. in biopolishing of denims, Biotechnol. Bioeng., 96(1):48-56.). [0003] As the main polysaccharide product of plant photosynthesis, cellulose is the most abundant carbohydrate on earth and the largest renewable resource. According to reports, the global annual production of cellulose through photosynthesis is as high as 1.55×10 9 t, 85% of which have not been utilized by humans (Dunlap C., Chiang G.C., 1980, Utilization and r...

Claims

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Application Information

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IPC IPC(8): C12N15/56C12N9/42C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10C12P19/14
Inventor 黄日波杜丽琴庞浩韦宇拓韦航赵英丽
Owner GUANGXI ACAD OF SCI
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