Chinese loropetalum extract comprising quinate and glucoside compounds and pharmaceutical application thereof
A technology of extract and quinic acid, which is used in the treatment of bleeding diseases and the preparation of hemostatic drugs.
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Embodiment 1
[0038] Implementation Example 1: Preparation of the total extract of Roropetus alba and different polar parts
[0039] Take 20kg of dried medicinal materials of P. chinensis, cut into 2-3cm segments, soak and extract 3 times with 8-10 times acetone at room temperature, 5 days each time, combine the extracts, concentrate under reduced pressure to dryness, and obtain 902g of total extract of P. . Weigh 793.5 g of acetone extract, add 4 L of deionized water for ultrasonic dispersion, and add 4 L of petroleum ether for extraction 5 times. The water layer was evaporated to dryness to obtain 564.5 g of the water-soluble fraction. Add 2L of deionized water to dissolve, apply HP20 macroporous resin after filtration, the ratio of resin column diameter to height is 1:8, use 10%, 20%, 30%, 40%, 50%, 70% of 2 times BV after sample loading 95% ethanol-water gradient elution, collect 20%, 30%, 40% ethanol eluate and evaporate to dryness under reduced pressure to obtain 20%, 30%, 40% ethan...
Embodiment 2
[0040] Implementation example 2: preparation and spectral analysis of each monomeric compound
[0041] Take 20kg of dried medicinal materials of P. chinensis, and use the method of Example 1 to prepare the elution site of 30% ethanol of P. spp. Take 30.7g of the obtained 30% ethanol elution fraction, dissolve it in 20ml water, filter and use reverse phase C 18 Silica gel column chromatography, gradient elution with different concentrations of aqueous methanol (10%, 20%, 30%, 40%, 50%, 70%, 95%), divided into sections F1-F46. Sections F19-20 are combined and recorded as component B, and then reversed phase C 18 Silica gel column chromatography, gradient elution with different concentrations of aqueous methanol (5%, 10%, 15%, 20%, 25%, 30%, 40%, 50%, 100%), divided into B1-B58. Where B8-B10 are combined, use LH-20 column chromatography, elute with methanol, and divide into B-8-1 to B-8-58, where components B-8-16 to B-8-26 are combined, and use silica gel Column chromatograph...
Embodiment 3
[0054] Implementation Example 3: Evaluation of the hemostatic effect of the total extract of Roropetus alba, different polar parts and monomeric compounds.
[0055] 1. The impact of the total extract of Example 1 on the bleeding time and bleeding amount by rat tail docking method
[0056] Experimental animals: Wistar rats, half male and half male, weighing 180g±10g, purchased from Beijing Weitong Lihua Company, and bred in the experimental animal center of the unit.
[0057] Test sample: the total extract of Example 1, dissolved with physiological saline.
[0058] Experimental method and results: 18 Wistar rats were randomly divided into 3 groups, 6 rats in each group. After the tail was docked 3cm from the root of the tail, intraperitoneal injection was administered, and the bleeding stop time and bleeding volume were observed; or intraperitoneal injection for 30 minutes Finally, the tail was docked at 3 cm from the root of the tail, and the bleeding stop time and bleeding v...
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