Novel Gus dyeing liquid and preparation method and application thereof
A preparation method and dyeing solution technology, applied in the field of plant genetic engineering, can solve problems such as defective selection of reagents, and achieve the effects of good dyeing effect, simple preparation process and saving test time.
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[0037] Embodiment: Take the preparation of 100 milliliters of x-gluc as an example
[0038] The container used for constant volume in the preparation process is a 100mL volumetric flask. The containers used to preserve the solutions are all 100mL triangular flasks. After all the solutions are prepared, the mouths of the triangular flasks will be sealed with parafilm.
[0039] Step 1: Preparations
[0040] 1. Prepare K 3 [Fe(CN) 6 ] Potassium ferricyanide solution: the required concentration is 50mM;
[0041] Specific method: Weigh 1.647g (gram) and dilute to 100mL sterile deionized water.
[0042] 2. Prepare K 4 [Fe(CN) 6 ] Potassium ferrocyanide: The required concentration is 50mM.
[0043] Specific method: Weigh 2.112g (grams) and dilute to 100mL (milliliters) sterile deionized water.
[0044] 3. Prepare PBS phosphate buffer:
[0045] First prepare liquid A: the composition of liquid A is NaH 2 PO 4 2H 2 O Sodium dihydrogen phosphate dihydrate, weigh 3.12g (grams...
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