Application of 6-hydroxy kaempferol-3-oxo-beta-glucoside in preparation of anti-cancer medicines
A technology of glucoside and anticancer drugs, which is applied in the application field of 6-hydroxykaempferol-3-oxo-β-glucoside in the preparation of anticancer drugs, and can solve the problem of 6-hydroxykaempferol-3- Oxygen-β-glucoside anti-tumor reports and other issues, to achieve strong pharmacological effects
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Embodiment 1
[0018] 6-Hydroxykaempferol-3-oxo-β-glucoside inhibits the proliferation of cancer cells:
[0019] The proliferation inhibitory effect of the compound on 8 kinds of tumor cell lines was measured by standard MTT colorimetric method. The tested tumor cell lines are human liver cancer cell line HepG2, gastric cancer cell line SGC-7901, breast cancer cell line MCF-7, cervical cancer cell line HeLa, bile duct cancer cell line QBC-939, esophageal cancer cell line EC9706, colon cancer cell line SW480 , glioma cell line C6, and human normal liver cells HL-7702 as a control.
[0020] The specific steps are: take each cell in logarithmic growth phase and in good condition, add 0.25% trypsin to digest, make a single cell suspension, count on a cell counting plate, and inoculate 100 μl per well in a 96-well plate, each well The number of cells is controlled at 5×104 / ml, 37°C, 5% CO2 incubator for overnight culture, and the prepared 6-hydroxykaempferol-3-oxygen-β-glucoside of different con...
Embodiment 2
[0023] Morphological Observation of Cells
[0024] DAPI is a very specific DNA staining agent, it can pass through the cytoplasm of the cell and its nuclear membrane very quickly, making DNA stained, and can emit blue fluorescence when observed under a fluorescent microscope. Liver cancer HepG2 cells and gastric cancer SGC-7901 cells were inoculated in 6-well plates. After the cells adhered to the wall and grew well, they were treated with 50 μg / ml 6-hydroxykaempferol-3-oxo-β-glucoside for 48 hours, and treated with 0.1% DMSO was used as the control group, and the cells were collected, washed with pre-cooled phosphate buffer solution (20 mM PBS, pH 7.2) and resuspended in fixative solution. After 15 minutes, the suspension was centrifuged at low speed to collect the precipitate, washed twice with buffer solution, and then the cell suspension was dropped onto a glass slide, stained with 2 μg / ml DAPI in the dark for 15 minutes, and the morphological changes of the nuclei were ob...
Embodiment 3
[0026] Apoptosis rate of HepG2 cells and SGC-7901 cells detected by flow cytometry
[0027] In order to detect the effect of 6-hydroxykaempferol-3-oxo-β-glucoside on the apoptosis of liver cancer HepG2 cells and gastric cancer SGC-7901 cells, 50 μg / ml 6-hydroxykaempferol-3-oxo-β- Glucoside was applied to HepG2 cells and SGC-7901 cells respectively. After 48 hours, they were stained with Annexin V / FITC and PI, and 0.1% DMSO was used as the control group. Cells were collected and detected by flow cytometry. The result is as image 3 As shown, A is the control group cells, and its early apoptosis rate is 3.3%, B-C is the experimental group, and its early apoptosis rate is significantly higher than that of the control group, respectively 32.4% and 17.2%. It can be seen that the compound can obviously induce Apoptosis of liver cancer HepG2 cells and gastric cancer SGC-7901 cells.
[0028] The above results show that 6-hydroxykaempferol-3-oxo-β-glucoside can very effectively inhib...
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