70 results about "Atractylenolide I" patented technology

The invention discloses atractylenolide extract having antiparkinsonian effect, a preparation method and application thereof. Weight percentages of atractylenolide I, atractylenolide II and atractylenolide III in the atractylenolide extract are larger than 5%, 10% and 20% respectively, and purity of total lactone is larger than 35%. The application refers to that the atractylenolide extract is used to prepare antiparkinsonian drug. The preparation method includes that oxidant is added into white atractylode rhizome powder, and atractylone in white atractylode rhizome volatile oil is oxidized into atractylenolide compounds, so that content of atractylenolide is increased; zinc particles are added into an extraction solution to protect generated compounds such as atractylenolide from being further oxidized and degraded. The preparation method is simple, convenient and practical, easy to operate and more suitable for industrial production, and has remarkable progressiveness and practical value.

The invention relates to a content determination method for multiple components in a Yupingfeng preparation. The method comprises the steps of: (1) taking the Yupingfeng preparation, crushing or not crushing it, then performing precise weighing, adding methanol to conduct reflux extraction for 1-3h, carrying out filtration and concentration, then adding methanol to a constant volume, thus obtaining a test solution; (2) precisely weighing the reference substance prim-o-glucosylcimifugin, calycosin-7-glucoside, cimifugin, 5-O-methylvisammioside, sec-o-glucosylhamaudol, calycosin, formononetin, atractylenolide III, atractylenolide I and atractylenolide II respectively, and adding methanol to perform dissolving to a constant volume, thus obtaining a mixed reference solution; (3) conducting determination: precisely sucking the test solution and the mixed reference solution respectively, injecting them into a high performance liquid chromatograph, carrying out gradient elution under certain mobile phase condition, and performing multi-wavelength simultaneous monitoring, thus obtaining the content. The method provided by the invention can accurately determine the content of 10 components in the Yupingeng preparation, and can objectively, comprehensively and sensitively reflect the quality condition of the Yupingfeng preparation.

The invention discloses a method for identifying rhizoma atractylodis macrocephalae by adopting a thin layer chromatography. The method comprises the following steps of: taking three target compounds of atractylone, atractylenolide I and atractylenolide III as comparison products, preparing a comparison product solution by using methanol and ultrasonically extracting the rhizoma atractylodis macrocephalae by using methanol to prepare a sample solution; sucking the comparison product solution and the sample solution, respectively applying samples on the same silica gel G thin-layer plate, carrying out secondary expansion by respectively taking petroleum ether-ethyl acetate with the temperature of 60-90 DEG C and cyclohexane-ethyl acetate as expanding agents, taking out and airing, carrying out identification by visually inspecting under the conditions of natural lights and the wavelength of 365nm after color development by adopting a sulfuric acid ethanol solution, and considering the sampling medicinal material which displays same color spots as the rhizoma atractylodis macrocephalae at the same Rf (Radio frequency) position of the three comparison products. The identifying method has strong specificity as well as simple and convenient operation, can be used for accurately identifying the rhizoma atractylodis macrocephalae and definitely distinguishing the rhizoma atractylodis macrocephalae from easily-confused product-rhizoma atractylodis.

The invention relates to the field of traditional Chinese medicine detection technology, in particular to a characteristic spectrum construction method and a quality detection method for bighead atractylodes rhizomes. According to the characteristic spectrum construction method for bighead atractylodes rhizomes, a characteristic spectrum of effective ingredients, namely atractylenolide I, atractylenolide II, atractylenolide III and atractylon of bighead atractylodes rhizome medicinal materials, bighead atractylodes rhizome herbal pieces and bighead atractylodes rhizome herbal pieces stir-bakedwith bran are analyzed through efficient liquid chromatographic analysis, chromatographic peaks of the characteristic spectrum are completely displayed, uniformity is good, the characteristic chromatographic peaks are completely separated, the quantity of impurities is small, a contrast characteristic spectrum is established according to the characteristic spectrum, and meanwhile the content of atractylenolide I and the content of atractylenolide III are determined. In combination with thin-layer chromatography identification and heavy metal and harmful element determination, the comprehensive quality detection method for bighead atractylodes rhizomes with effectiveness and safety is provided, so that quality detection on bighead atractylodes rhizomes, processed products of bighead atractylodes rhizomes and relevant products is realized. Moreover, the quality detection method has the advantages of being simple, fast, stable, reliable, high in precision, good in reproducibility, easy to grasp and the like.

The invention belongs to the technical field of medicines, and particularly relates to application of pectolinarin in preparation of a medicine for treating glycolipid metabolism diseases and a medicinal composition. According to the application of the pectolinarin in preparation of the medicine for treating the glycolipid metabolism diseases, experiments prove that the pectolinarin has the effects of reducing blood sugar of mice suffering from glycolipid metabolism disorder, regulating blood fat, reducing weight and reducing abdominal girth, can also promote L cells of intestinal tracts of the mice to secrete hypoglycemic hormone GLP-1 to achieve the effect of enhancing the insulin sensitivity, and can effectively treat the glycolipid metabolism diseases. The invention also provides the medicinal composition containing the pectolinarin. The medicinal composition comprises the pectolinarin and atractylenolide I. Compared with the pectolinarin, the medicinal composition has the advantages that the blood sugar is reduced, the blood fat is regulated, the effect of promoting the intestinal L cells to secrete the hypoglycemic hormone GLP-1 is more remarkable, and the medicinal composition has a good application prospect in the aspect of treating the glycolipid metabolism diseases.

The invention provides application of atractylenolide in preparation of an antidepressant drug, and relates to the field of medicine. In-vitro activity tests prove that atractylenolide I and atractylenolide III have the better protection activity on corticosterone-damaged PC12 cells, and the mechanism is related to inhibition of neuron apoptosis; in-vivo test results show that atractylenolide I and atractylenolide III can significantly shorten the immobility time of a mouse forced swimming test and a mouse tail suspension test. Experiments prove that atractylenolide I and atractylenolide III have the significant antidepressant effect and can be used for preparing the drug for preventing and treating a depressive disorder.

The invention discloses a method of single time measurement of atractylenolide and atractylon. The procedure includes preparing mobile phase resolution A and B made of chromatographic pure methanol, acetonitrile and redistilled water, preparing the mixed control solution made of atractylenolide I, atractylenolide III and atractylon, preparing sample solution, testing the three absorption peaks of control solution and samples at three different time respectively by chromatograph of liquid according to the setting gradient of mobile phase A and B and the time, and calculating the content of atractylenolide I, atractylenolide III and atractylon according to the formula X=(peak area of sample is multiplied by density of control then is divided by peak area of control and is multiplied by density of sample) is multiplied by 100 percent. The invention can determine the content of atractylenolide I, atractylenolide III and atractylon in atractylodes macrocephala by only one sample injection. Thus the operation is simplified, the error of multiple sample injection is reduced and analysis of the constituent ration of the three ingredients is convenient. The invention is of important academic value and meaningful in practical application.

The invention provides a method for determining chemical components in traditional Chinese medicines by liquid chromatography-mass spectrometry. The method comprises the steps of preparing a mixed standard solution and a tested traditional Chinese medicine solution, and carrying out sample injection determination by using a liquid chromatography and mass spectrometry combined technology; and by comparing an accurate molecular weight, retention time with the chemical components in the mixed standard solution, carrying out qualitative and quantitative determination on the corresponding chemicalcomponents in a traditional Chinese medicine extracting solution. The invention further provides application of the method, and it can determine that the traditional Chinese medicines comprise the following chemical components: one or more of benzoylaconitine, benzoylmesaconine, atractylodes macrocephala lactone I, atractylodes macrocephala lactone II, atractylodes macrocephala lactone III, codonopsis alkynyl glycoside, liquiritin, liquiritigenin, 8-gingerol and 10-gingerol. By the method, simultaneous detection and quantification of one or more of the 10 chemical components in the traditionalChinese medicines can be realized. The method has the advantages of rapid and efficient determination method, high sensitivity and simple and feasible determination method, and a methodological basisis provided for the quality control of the traditional Chinese medicines.

The invention discloses a resuscitation method of CAR-T cells. The resuscitation method comprises the following steps: taking out a cryopreservation bag loaded with CAR-T cells and cryopreservation liquid from liquid nitrogen, adding cryopreservation liquid preheated at 36-40 DEG C, shaking the cryopreservation bag for thawing under the condition of 36-40 DEG C water bath for 1-2 minutes, performing centrifuging, and discarding supernate; and adding resuscitation liquid preheated at 36-40 DEG C, performing mixing, performing centrifuging, discarding supernate, and resuspending the cells by using a cell culture medium, wherein the resuscitation liquid comprises the following components of 10-50 [mu] g/L L-proline, 10-100 mg/L sodium selenite, 2.0-2.2 mg/L sodium pyruvate, 0.2-0.5 mg/L f NaOH, 0.2-0.4 mg/L magnesium chloride, 15-25 mg/L astragaloside, 10-50 mg/L atractylodes macrocephala lactone II, 15-30 mg/L sodium diatrizoate and the balance the cell culture medium. By adopting the resuscitation method disclosed by the invention, the motility rate, the multiplication capacity and tumor cell removal capacity of cells can be well maintained. And the method is simple and convenient to operate, low in cost and beneficial to industrial use.

The invention belongs to the technical field of traditional Chinese medicine component detection and provides a method for determining the content of nineteen components in radix stephaniae tetrandraeand astragalus membranaceus soup by ultra-high performance liquid chromatography-tandem mass spectrometry. The method comprises the following steps of pespectively preparing a test solution, a reference solution and a mixed standard solution; detecting the mixed standard substance solution by ultra-high performance liquid chromatography-tandem mass spectrometry, drawing a standard curve and a test solution, performing mass spectrometry positive and negative ion mode analysis by ultra-high performance liquid chromatography-tandem mass spectrometry, and obtaining the contents of nineteen components in the radix stephaniae tetrandrae and astragalus membranaceus soup according to the standard curve. The method is high in sensitivity and good in reproducibility; analysis accuracy and reliability, 19 chemical components in the radix stephaniae tetrandrae and radix astragali soup can be quantitatively detected at the same time; formononetin, formononetin, calycosin-7-one, calycosin-7-one, pterocarpan, pterocarpan, isoflavane, liquiritin, glycyrrhetinic acid, tetrandrine, tetrandrine, atractylodes lactone I, atractylodes lactone II I, isoflavane, astragaloside I, astragaloside II, astragaloside III and astragaloside IV.

The invention relates to the field of medicines, and provides an application of atractylenolide II in preparation of an anti-renal fibrosis medicine, and an effective anti-renal fibrosis effect can beachieved by taking an atractylenolide II monomer as an effective component. The invention further provides an anti-renal fibrosis medicine which comprises the atractylenolide II and pharmaceutic adjuvants. Researches show that the atractylenolide II has a good effect of treating renal fibrosis.

Disclosed is a composition comprising a plurality of active botanical ingredients, each predominantly associated with an in-use beneficial effect on an animate or inanimate substrate, where said composition comprises: (i) a first aqueous extract of a material derived from a first plant, said first extract comprising a first amount of a first active botanical ingredient; (ii) a second aqueous extract of a material derived from a second plant whose genus is not the same as the first plant, said second extract comprising a first amount of a second active botanical ingredient; (iii) an additional amount of said first active botanical ingredient; and (iv) an additional amount of said second active botanical ingredient; where each said additional amount is included by spiking said composition or the corresponding aqueous extract selectively with said first and said second active botanical ingredients, such that ratio of said first amount of said first active botanical ingredient to its corresponding additional amount and ratio of said first amount of said second active botanical ingredient to its corresponding additional amount in the composition is from 1:1 to 1:3000 parts by weight, wherein said composition is substantially free of non-aqueous extracts of any material derived from said first or said second plant wherein said first plant is Atractylodes macrocephala and said first active botanical ingredient is atractylenolide I, II or III and further wherein said second plant is Glycyrrhiza uralensis, Glycyrrhiza inflata or Glycyrrhiza glabra and said second active botanical ingredient is licochalcone A or glabridin.

The invention relates to a quality control method of a substance reference of a Linggui curcuma zedoary decoction. The method comprises an HPLC content determination method for simultaneously determining four components of liquiritin, cinnamic acid, ammonium glycyrrhizinate and atractylenolide III of the substance reference of the Linggui curcuma zedoary decoction and an HPLC characteristic chromatogram of the substance reference of the Linggui curcuma zedoary decoction. According to the structural characteristics of the active ingredients contained in the Linggui curcuma zedoary decoction, the optimal mobile phase composition, gradient elution procedure, flow velocity, detection wavelength, chromatographic column, column temperature and other analysis conditions are screened out through a large number of experiments, the method for detecting the quality of the Linggui curcuma zedoary decoction can comprehensively, objectively and accurately detect and evaluate the quality of the Linggui curcuma zedoary decoction material reference and the preparation thereof, has important significance for ensuring the clinical curative effect of the Linggui curcuma zedoary decoction material reference, and provides a reliable quality attribute research method and evaluation system for the classical famous prescription Linggui curcuma zedoary decoction material reference and the preparation of the classical famous prescription Linggui curcuma zedoary decoction material reference.

The invention discloses a quality detection method of a Shouhui bowel-relaxing capsule and belongs to the field of analysis of traditional Chinese medicinal preparations. The quality detection methodadopts a thin-layer chromatography to identify bighead atractylodes rhizome in the capsule and simultaneously adopts an HPLC to determine the contents of atractylenolide I, atractylenolide II and atractylenolide III in the capsule. The quality detection method disclosed by the invention is stable and reliable, strong in specificity, good in reproducibility and capable of fully and effectively controlling the quality of the euphorbia pulcherrima bowel-relaxing capsule, ensuring the safety and the effectiveness of clinical medication and better meeting the needs of patients and the market.

The invention provides a quality evaluation method for Chinese herbal compound aconite lizhong decoction for treating gastric ulcer. The method defines content limits of 10 chemical components of benzoylaconitine, benzoylmesaconine, atractylenolide I, atractylenolide II, atractylenolide III, lobetyolin, liquiritin, liquiritigenin, 8-gingerol and 10-gingerol in the Chinese herbal compound aconite lizhong decoction; the content is determined through a liquid chromatography-mass spectrometry method; and meanwhile, the 10 chemical components are detected simultaneously. The method is used for evaluating the quality of the Chinese herbal compound aconite lizhong decoction for treating gastric ulcer. The content of the 10 chemical components in the Chinese herbal compound aconite lizhong decoction is detected through the liquid chromatography-mass spectrometry method, so that the 10 chemical components can be detected simultaneously and quantified through one injection; and the method is fast in determination, efficient, high in flexibility and simple and feasible in detection, and can quickly evaluate the quality of the Chinese herbal compound aconite lizhong decoction.

The invention discloses a traditional Chinese medicine composition with an anti-cancer effect and an anti-cancer traditional Chinese medicine implant, and relates to a traditional Chinese medicine composition and an implant. The problems that an existing traditional Chinese medicine composition is low in lung cancer cell proliferation inhibition rate and unstable in implant release rate are solved. The traditional Chinese medicine composition is prepared from a traditional Chinese medicine extract, atractylenolide I and crocin. The preparation of the implant comprises the steps: dissolving the PLGA and the mannitol in an organic solvent, adding the traditional Chinese medicine composition, performing drying, performing forming, performing sub-packaging and performing sterilizing. The auxiliary release device containing the anti-cancer traditional Chinese medicine implant is composed of a medicine storage bin, foamed plastic, the flaky anti-cancer traditional Chinese medicine implant and a mesh, the traditional Chinese medicine composition can inhibit proliferation of tumor cells and has low toxic and side effects, and the auxiliary release device ensures that the release rate is constant.