Method for identifying rhizoma atractylodis macrocephalae by adopting thin layer chromatography

Abstract

The invention discloses a method for identifying rhizoma atractylodis macrocephalae by adopting a thin layer chromatography. The method comprises the following steps of: taking three target compounds of atractylone, atractylenolide I and atractylenolide III as comparison products, preparing a comparison product solution by using methanol and ultrasonically extracting the rhizoma atractylodis macrocephalae by using methanol to prepare a sample solution; sucking the comparison product solution and the sample solution, respectively applying samples on the same silica gel G thin-layer plate, carrying out secondary expansion by respectively taking petroleum ether-ethyl acetate with the temperature of 60-90 DEG C and cyclohexane-ethyl acetate as expanding agents, taking out and airing, carrying out identification by visually inspecting under the conditions of natural lights and the wavelength of 365nm after color development by adopting a sulfuric acid ethanol solution, and considering the sampling medicinal material which displays same color spots as the rhizoma atractylodis macrocephalae at the same Rf (Radio frequency) position of the three comparison products. The identifying method has strong specificity as well as simple and convenient operation, can be used for accurately identifying the rhizoma atractylodis macrocephalae and definitely distinguishing the rhizoma atractylodis macrocephalae from easily-confused product-rhizoma atractylodis.

Description

technical field [0001] The invention relates to a method for identifying Atractylodes macrocephala medicinal material, in particular to a method for identifying Atractylodes macrocephala medicinal material by thin-layer chromatography. The invention belongs to the technical field of identifying traditional Chinese medicinal materials. Background technique [0002] The traditional Chinese medicine Atractylodes macrocephala is the dried rhizome of Atractylodes macrocephala (Rhizoma atractylodis macrocephala Koidz.), which is recorded in "Chinese Pharmacopoeia" 2010 edition, part one. It is a well-known traditional multi-purpose commonly used traditional Chinese medicine, and is one of the "Eight Flavors of Zhejiang". It has the effects of invigorating the spleen and replenishing qi, drying dampness and promoting diuresis, antiperspirant and preventing miscarriage. It is planted in many provinces in China, and Zhejiang Province is one of the main producing provinces. The annu...

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Problems solved by technology

The thin-layer identification method with a single component as the index has shortcomings, mainly because the identification basis is not sufficient, and it cannot accurately distinguish Atractylodes macrocephala and its confusing product-Atractylodes atractylodes

[0004] In view of the fact that there is no TLC identification method using the three components as indicators, there is no identification index for Atractylodes atractylodes under the item of Atractylodes identification in the Pharmacopoeia (2010 Edition, Part One)

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