Method for determining content of effective components of atractylodes macrocephala lactone III and I in atractylodes macrocephala koidz

Abstract

The invention relates to the technical field of detection, and in particular relates to a method for determining the content of effective components of atractylodes macrocephala koidz lactone III andI in atractylodes macrocephala koidz. The preparation method comprises the steps of grinding bighead atractylodes rhizome into powder, and adding ethyl acetate for soaking; evaporating to dryness after suction filtration, adding methanol for dissolving, and fixing the volume; filtering by using a filter membrane, and taking the subsequent filtrate; precisely weighing reference substances of atractylodes macrocephala koidz lactone III and I; adding methanol to prepare a solution containing 0.1 mg of methanol per 1ml; precisely sucking 10 [mu] L of the reference solution and 10 [mu] L of the test solution, and respectively injecting into a liquid chromatograph for determination; and recording a chromatogram, and calculating the content of atractylodes macrocephala koidz lactone III and I byan external standard method. According to the method, the content of atractylodes macrocephala koidz lactone III and I is determined through a liquid chromatograph, chromatograms are recorded, the content of atractylodes macrocephala koidz lactone III and I is calculated through the external standard method, the operation is easy, convenient and rapid, meanwhile, under set chromatographic conditions, the peak area is determined, the sample precision, reproducibility, stability and recovery rate are good, and evaluation standards are met.

Description

technical field [0001] The invention relates to the technical field of detection, in particular to a method for determining the content of atractylodes lactone III and I, the active ingredients in Atractylodes macrocephala. Background technique [0002] Atractylodes macrocephala is a commonly used and important bulk Chinese herbal medicine, which has the functions of invigorating the spleen and stomach, drying dampness and diuresis, antiperspirant and preventing miscarriage. The rhizome of Atractylodes Rhizoma Atractylodes Rhizome contains volatile oil, and the main components in the oil are atractylodes ketone, atractylodes alcohol, and atractylodes lactone. Certain curative effect. Atractylodes macrocephala has a wide range of uses. In addition to medical prescriptions, it is also an important raw material for more than 40 kinds of Chinese patent medicine preparations. [0003] Atractylodes lactone is a compound in Atractylodes macrocephala, and Atractylodes lactone III ...

AI Technical Summary

Problems solved by technology

[0003] Atractylodes lactone is a compound in Atractylodes macrocephala, and Atractylodes lactone III and Atractylodes lactone I are the main lactone components in Atractylodes macrocephala. At present, the determination methods of atractylodes lactone in Atractylodes macrocephala mostly use one of CC, HPLC and mass spectrometry Or several active ingredients, the quality control of Atractylodes macrocephala has not yet formed a unified standard system, therefore, it is of great significance to establish a stable, controllable, accurate and efficient determination method for the content of Atractylodes lactone III and Atractylodes lactone I

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