128 results about "Alpha-Ketoglutaric acid" patented technology

The invention relates to a preparation method of crystal L-arginine alpha-ketoglutarate, comprising the following steps: preparing water solution of L-arginine, and slowly adding alpha-ketoglutaric acid into the water solution, wherein the mol ratio of L-arginine and alpha-ketoglutaric acid is 1:1 or 2:1; reducing pressure and concentrating the alpha-ketoglutaric acid at 50-80 DEG C after complete dissolution, removing water accounting for 20-60 percent of the total volume and cooling to 20-30 DEG C; slowly adding an organic hydrophilic solvent or a mixed solvent of a polar hydrophilic solvent and a non-polar hydrophilic solvent; and carrying out temperature reduction, crystallization, filtering, washing and vacuum drying to obtain the crystal L-arginine alpha-ketoglutarate. Compared with the prior art, the invention has the advantages of reasonable process, simple steps, easy operation, good crystal form of the obtained product, difficult caking, convenient transportation and use, high purity (more than or equal to 98 percent) and high yield (80-86 percent) of the product, and the like, and is suitable for industrialization production.

The invention relates to a feed capable of enhancing growth performance and protein deposition of a carp, solving the problem that protein deposition rate of the existing feed is low. The feed capable of enhancing growth performance and protein deposition of carp comprises the following components in parts by weight: 17-19 parts of fish meal, 29-40 parts of soybean meal, 32-45 parts of middlings, 0.8-1.1 parts of soybean lecithin, 2-3 parts of fish oil, 1-2 parts of soybean oil, 1.5-2.5 parts of monocalcium phosphate, 0.4-0.6 part of choline, 0.2-0.5 part of lysine, 0.1-0.25 part of sulfur-containing amino acid, 0.4-0.6 part of premix and 0.7-1.6 parts of alpha-ketoglutaric acid. The feed provided by the invention is applied to the field of carp cultivation.

A method for producing an L-amino acid of glutamate family such as L-glutamic acid is provided. An L-amino acid of glutamate family is produced by culturing a coryneform bacterium having an ability for producing an L-amino acid of glutamate family, which has been modified so that the activity of an alpha-ketoglutaric acid (alpha-KG) uptake carrier is increased, in a medium, and collecting the L-amino acid of glutamate family from the medium.

The invention discloses a method for extracting alpha-ketoglutaric acid from fermentation liquor. The method comprises the following steps: heating fermentation liquor containing the alpha-ketoglutaric acid for sterilization and decolorization, and drying mycoprotein; adding Ca(OH)2 into a filtration liquid, and performing centrifugal separation to obtain calcium 2-oxoglutarate and Ca(OH)2; adding H2SO4 into the calcium 2-oxoglutarate and Ca(OH)2, and performing centrifugal separation to obtain CaSO4 sediments; enabling the filtration liquid to flow into an ion exchange column for adsorption, and adding activated carbon into eluant for decolorization for 30 min; performing pressure reduction evaporation on the high-concentration alpha-ketoglutaric acid solution to obtain alpha-ketoglutaric acid crystals, and performing centrifugal separation to obtain an alpha-ketoglutaric acid crude product, washing the alpha-ketoglutaric acid crude product with methanol, removing impurities through washing, and performing centrifugation to remove methanol, so as to obtain the alpha-ketoglutaric acid, wherein the volume ratio of the alpha-ketoglutaric acid crude product to the methanol is 5:1. Through the adoption of the method, the problems in the prior art that pyruvic acid as a by-product during the extraction process of the alpha-ketoglutaric acid cannot be effectively separated and the discharge amount of waste liquid is high are solved.

The invention provides a method and a special-purposed strain used for producing alpha-ketoglutaric acid. Lichtheimia corymbifera D1 provided by the invention has a collection number of CGMCC NO.4974. The lichtheimia corymbifera D1 provided by the invention has good strain stability, good alpha-ketoglutaric acid production capacity, relatively low fermentation cost (substrates have wide source and low cost), and good industrialized application prospect. The invention also optimizes the fermentation conditions of lichtheimia corymbifera D1. The method provided by the invention is used for producing alpha-ketoglutaric acid, and has good scientific research value and industrial application prospect.

The invention relates to a preparation method of hydroxyectoine. According to the preparation method, an ectoine-containing feed liquid is adopted as a substrate, Escherichia coli with ectonine hydroxylase activity and alpha-ketoglutaric acid are added, and an enzymatic reaction is performed under conditions that the pH is 7.0-8.0 and the temperature is 37-45 DEG C to synthesize the hydroxyectoine. According to the method, the hydroxyectoine with the higher added value is synthesized with the ectoine-containing feed liquid, prepared with a fermentation method, used as the substrate, and the method has the advantages that the raw material source is wide, the production cost is low and the like.

The invention aims at providing a method for synthesizing alpha-ketoglutaric acid by a biological conversion method. According to the method, L-glutamic acid is used as a substrate; in a conversion culture solution obtained from kluyveromyces marxianus ATCC36534 through being cultured via a conversion culture substrate, alpha-ketoglutaricdehydrogenase inhibitors are added; the synthesis conversion culture is performed under the oscillation conditions of 25 to 30 DEG C and 200 to 250 r/min; after the synthesis conversion culture is completed, the alpha-ketoglutaric acid is obtained through separating and purifying the synthesis conversion solution; the L-glutamic acid is converted into alpha-ketoglutaric acid by yeast cells in the growth state; the alpha-ketoglutaricdehydrogenase inhibitors are added; the further metabolism consumption is blocked, so that a great amount of alpha-ketoglutaric acid is accumulated in the culture medium; when the feeing concentration of the substrate L-glutamic acid is 50g/L, the mol conversion rate can reach 83.2 percent. The low-value L-glutamic acid is converted into high-value alpha-ketoglutaric acid; the large-scale industrial application can be realized; a production process has the advantages of short period, high conversion rate, small environment pollution and the like.

A method for improving adsorption of amino acids in a vertebrate, including mammal and bird, is included. The method comprises administering to a vertebrate, including mammal and bird, in a sufficient amount and/or at a sufficient rate to enable desire effect. AKG, AKG derivatives or metabolites, AKG analogues or mixture thereof. Also conteplated is a method for decreasing adsorption of glucose in a vertebrate, including mammal and bird, in the need thereof, AKG, AKG derivates or metabolites, AKG analogues or mixture thereof, for decreasing glucose adsorption as well as compositions for use in treatment.

The invention provides a multifunctional biological adsorbent, and a preparation method thereof comprises the following steps: chitosan alpha-ketoglutaric acid is taken as a carrier, a heavy metal ion is taken as an imprinted template, an ionic imprinting material is obtained through the ionic imprinting technique, then the ionic imprinting material and magnetic nano-Fe3O4 are subjected to cross-linking reaction, a magnetic ionic imprinting material is obtained, and finally the surface of a Serratia marcescens thallus is coated with the magnetic ionic imprinting material through curing reaction. The obtained biological adsorbent integrates advantages of high adsorption with low cost, high selectivity with low pollution, high separating efficiency with low loss, and the like, also has advantages of magnetic materials and biological adsorbents, and can realize various functions such as magnetic separation, selective recognition, circulative recovery and the like; moreover, the method is simple, the cost is low, and the biological adsorbent is green and environment-friendly, avoids secondary pollution, and can be widely used for treatment of various specific heavy metal industrial wastewater, therefore, a novel material is provided for treatment of industrial wastewater.

The invention discloses a method for efficiently producing L-glutamate oxidase and belongs to the technical fields of fermentation engineering and enzyme engineering. In the method disclosed by the invention, the optimal induction condition for L-glutamate oxidase recombinant bacteria is inducted for 5h in the presence of 5g/L lactose at 25-30 DEG C; through researches on different feeding modes of batch feeding fermentation, a manner of combining index feeding with Do-stat is beneficial to increase for a bacteria density; through optimization for induction condition of batch fermentation, inducing lasts for 12h in the presence of 10g/L lactose under the condition of a logarithmic phase OD600 of 40, and the highest enzyme activity reaches 156.1U/mL; high-expression recombinant wet bacteria are added in buffer solution containing 110g/L of glutamic acid with a pH value of 8.0, and converted for 24h at 37 DEG C to produce 107.9g/L of alpha-ketoglutaric acid, the conversion rate is 90% or above, and the needed bacterium solution amount is only 1/50 of that of shake-flask fermentation.

A method for improving adsorption of amino acids in a vertebrate, including mammal and bird, is included. The method comprises administering to a vertebrate, including mammal and bird, in a sufficient amount and/or at a sufficient rate to enable desire effect. AKG, AKG derivatives or metabolites, AKG analogues or mixture thereof. Also conteplated is a method for decreasing adsorption of glucose in a vertebrate, including mammal and bird, in the need thereof, AKG, AKG derivates or metabolites, AKG analogues or mixture thereof, for decreasing glucose adsorption as well as compositions for use in treatment.

The invention relates to a synthesis method for 4-hydroxyisoleucine. The method comprises the following steps: firstly, constructing recombinant expression plasmids for expressing L-isoleucine-4-hydroxylase and L-glutamate oxidase simultaneously; adding two types of substrates including L-glutamic acid and L-isoleucine into a constructed catalytic reaction system; taking the substrates as precursors of a catalytic reaction and synthesizing the 4-hydroxyisoleucine. After the catalytic reaction is performed for 4h, the conversion rates of the L-glutamic acid and the L-isoleucine are 97.01 percent and 98.45 percent respectively. By replacing alpha-ketoglutaric acid with the L-glutamic acid and utilizing the L-glutamic acid as the precursor for synthesizing the 4-hydroxyisoleucine, the 4-hydroxyisoleucine can be efficiently produced, the production cost is low and industrial production is convenient to realize.