The invention provides a preparation method of an acellular collagen matrix membrane, and the method comprises the following steps: performing pretreating to obtain a mammalian membrane tissue, and then carrying out repeated freeze-thawing treatment, acid-base treatment, gradient
dehydration treatment,
organic matter degreasing treatment and hypotonic / hypertonic treatment on the mammalian membrane tissue, thereby effectively removing tissue
immunogenicity, retaining an ordered
collagen fiber structure, and improving the decellularized collagen matrix membrane; then performing
drying and sterilizing to obtain an acellular collagen matrix membrane, wherein the decellularized collagen matrix membrane has excellent flexibility,
biocompatibility and soft and
hard tissue regeneration promoting performance, has a smooth-surface and rough-surface double-layer bionic structure, can retain a natural three-dimensional pore
scaffold structure similar to the tissue, retains a collagen structure, and has good water absorption and
thermal denaturation stability; source materials are easy to obtain, the cost is low, the decellularized collagen matrix membrane has a stronger
barrier function and a space maintaining function, and the surface appearance, the
elastic modulus, the
porosity and the pore size of the decellularized collagen matrix membrane can be regulated and controlled through different freeze-thaw treatment
modes.