The invention discloses a mushroom cultivation charging mulcher which comprises a support, a feeding hopper and a conveying belt. The feeding hopper is mounted on the support, a feeding opening is formed in the bottom of the feeding hopper, and the conveying belt is placed below the feeding hopper and is used for conveying cultivation beds. The feeding hopper is provided with a first side plate and a second side plate which tilt relatively, the bottom of the first side plate is provided with a stopping part which extends to the second side plate in a curved mode, and the feeding opening is formed between the stopping part and the bottom edge of the second side plate. A roller which is arranged at the position, close to the feeding opening, in the feeding hopper in a rotatable matching mode, the roller presses close to the first side plate, and material dialing teeth are distributed on the peripheral face of the roller. The mushroom cultivation charging mulcher is simple in structure, low in manufacturing cost and convenient to popularize, the feeding opening which is obliquely arranged is used, the roller with the material dialing teeth is mounted on the side edge of the feeding hopper, caking and bridging of materials are prevented, the falling materials are distributed more evenly, the feeding opening with the adjustable size is arranged to be used for controlling the feeding speed, and the demand for charging of the cultivation beds is met.