The invention relates to a pharmaceutical composition containing 17 amino acids. The pharmaceutical composition comprises the following raw materials and auxiliary materials: proline, serine, alanine, isoleucine, leucine, tyrosine, glutamic acid, phenylalanine, arginine, lysine acetate, valine, threonine, histidine, tryptophan, methionine, acetylcysteine, glycine, sorbitol, and sodium bisulfite. The product is free of chlorine ion, and is wider in application crowd range; in the production, an acid or a base is not additionally added for adjusting the pH value; and the product is prepared according to the component ratio, and has the pH value of 5.5-7.0. Therefore, the pharmaceutical composition meets the pharmaceutical registering requirements that the material consumption is reduced and the production cost is lower, and has equivalent or better quality and stability as compared with the commercially available products.