106results about How to "Less blood" patented technology

The invention discloses a method for detecting multiple lipid-soluble vitamins in a blood sample simultaneously. The method comprises the steps of preprocessing a biological sample by a simple liquid-liquid extraction method, then carrying out chromatographic separation and mass spectrometric detection, respectively selecting a pair of qualitative ions and a pair of quantitative ions according to each vitamin, wherein the relative retention time and qualitative ion pairs of the various vitamins are taken as qualitative basis, and a standard curve for quantitation is formed by standard substances. Meanwhile, according to the method, three levels of quality control substances are used for inspecting the accuracy and effectiveness of the method, and the distortion of the detection result is avoided. According to the method, the purpose that six lipid-soluble vitamins in one serum sample can be simultaneously detected can be realized for the first time by applying an LC-MS technology, the influence caused by interferents can be alleviated, the operation is simple, convenient and rapid, the analysis time is only 4.8min, the throughput is high, the cost is low, the levels of lipid-soluble vitamins in a human body are effectively monitored, and the method has guiding significance on the reasonable and safe supplementation of vitamins, and is liable to clinical promotion and popularization.

A circulatory assist apparatus comprising: an inflatable pumping balloon having a proximal end joined to an elongated balloon catheter, the balloon catheter having a distal end joined to the pumping balloon and a proximal end, separated from the distal end by a length sufficient to extend from within a circulatory lumen to the outside of a patient's body, for receiving positive and negative pressure pulses from a pump to inflate and deflate the pumping balloon; and a radially expandable frame, mounted on one of a segment extending distally from the pumping balloon, the balloon catheter, and a sleeve tube surrounding the balloon catheter. The expandable frame is manipulate to expand within the circulatory lumen, and functions to space apart the inflatable balloon from the circulatory lumen, having a first diameter in a collapsed configuration for intraluminal delivery and a second, larger diameter in an expanded configuration achieved by said manipulation.

A field effect transistor-based rapid human tumor diagnosis apparatus comprises a field effect transistor biochip, a microfluidic channel sample packaging system and a set of electronic detection system. The semiconductor manufacturing technology is utilized to construct a source electrode, a drain electrode and a semiconductor channel between the source electrode and the drain electrode on a silicon substrate, silane reagent is covalently modified on the channel to serve as connecting molecules, which are connected with the specific antibody protein of a certain tumor marker, a microfluidic channel which is constructed with polydimethylsiloxane is then tightly attached and packaged on the silicon substrate, and finally, the source electrode and the drain electrode are connected with the signal detection system. By assaying the tumor marker in human blood, the tumor diagnosis apparatus can rapidly, accurately and early diagnose whether a patient suffers from a certain type of cancer, and is an integrated, high-flux and portable tumor diagnosis apparatus.

Belonging to medical diagnosis, especially the in vitro diagnosis field, the invention discloses a dry type biochemical immune mixed analysis system and a device thereof. The mixed analysis system includes a dry type biochemical and immune detection system able to individually or simultaneously complete biochemical and immune detection, and the system includes mixed type and parallel type two modes. The system device involved in the invention is combined by a support base plate, a reagent strip storage unit, a sample addition unit, a sample unit, a detection unit, and a circuit unit, and includes single reaction disk and double-reaction disk two forms. Specifically, the single reaction disk can complete biochemical and immune analysis mixedly, the double reaction disks correspond to biochemical and immune analysis procedures respectively, and the detection process of the two can be finished integratedly. According to the invention, between-sample pollution is effectively reduced, compared with single biochemical type or immune type, the system and the device greatly increase the number of detection items, and have the characteristics of fast detection speed and small blood collection amount, thus reducing the patient pain in the detection process.

Treatment or prevention methods are described wherein t-PA and C1-inhibtor are used together in order to minimize the hemorrhagic complications of tPA Preferably, C1-inhibitor is infused prior to treatment with t-PA, thereby allowing for a safer thrombolysis without the excessive and dangerous bleeding associated with the use of t-PA alone particularly in the treatment of ischemic stroke.

The invention relates to a kit, device and method for detecting the copy number of embryo chromosomes. By using the kit, device and method provided by the invention, the copy number of embryo chromosomes can be detected non-invasively and economically. The inventor finds that most embryo DNAs (deoxyribonucleic acids) in maternal plasma are 100bp-250bp segments and the ratio of each chromosome to the whole DNA is equal to the ratio of each chromosome to the DNA at any point or in any interval within the range of 100bp to 250bp in the maternal plasma. According to the method provided by the invention, the copy number of chromosomes to be detected can be determined by simply confirming which chromosome each DNA segment of the DNA at any point or in any interval within the range of 100bp to 250bp comes from, calculating the ratio of the number of DNA segments from the chromosomes to be detected to the number of DNA segments from reference chromosomes in all the DNAs at any point or in any interval within the range of 100bp to 250bp in the same sample, calculating the differences among the samples and determining according to the difference values.

The invention discloses a system for monitoring, collecting and detecting the biological information of human blood in real time, which comprises a sensor group, a central processor, a blood-specimen collecting system, a blood-specimen detecting system and a vein indwelling body, wherein the indwelling body which can be indwelled in a vein is adopted, the time interval of blood-specimen collection is set by the central processor, and a blood specimen is collected and transported to the blood-specimen detecting system by the blood-specimen collecting system. When applied to clinics, the system can be used for perform painless, controllable and uninterrupted monitoring, blood-specimen collection and blood-specimen detection for the human blood to obtain the more comprehensive information about the blood of patients and integrally and dynamically know the pathophysiologic states of human bodies in real time. The invention solves the technical difficult problems that the blood coagulation and the deposition of protein and other substances in the blood influence subsequent continuous monitoring and cause wounds to the patients and the like; and the system uses capillaries for collecting blood, has an extremely-small blood collecting quantity, can not damage the safety range of human health and has more important significance for guiding clinical treatment.

The invention discloses a full-automatic determining instrument of red blood cell osmotic fragility. The full-automatic determining instrument is based on the principle of light-proof turbidimetry and consists of a sample absorbing device, an automatic electromechanical device, a light detector and a circuit system, wherein the sample absorbing device is used for absorbing a blood sample through the instrument; the automatic electromechanical device is used for accomplishing various control actions in the automatic measurement of the instrument; the light detector is used for radiating reaction liquid to generate a permeable light signal; the circuit system is used for controlling various stepper motors, electromagnetic valves and peristaltic pumps, converting the light signal into an electric signal, amplifying the electric signal and converting the electric signal into a digital signal so as to carry out data processing analysis, data display and communication between the instrument and a PC (Personal Computer). The full-automatic determining instrument is convenient to operate, rapid in measuring speed, high in precision and good in repeatability, and the information of the red blood cell osmotic fragility of the blood sample can be obtained through full-automatic detection.

The invention discloses a multifunctional micro-fluidic detection chip. The detection chip comprises a chip body, wherein a sample injection cavity, a sample quantifying cavity, a sample overflow cavity, a diluent storage cavity, a diluent quantifying cavity, a diluent overflow cavity, a quantitative uniform mixing cavity, a reaction cavity and an air hole are formed in the chip body; a to-be-detected sample is injected into the sample injection cavity and enters the sample quantifying cavity through a micro-channel, and redundant samples enter the sample overflow cavity; diluent in the diluent storage cavity enters the diluent quantifying cavity through the micro-channel, and redundant diluent enters the diluent overflow cavity; the reaction cavity comprises one or more reaction chambersand a sample blank chamber; and after the sample in the sample quantifying cavity and the diluent in the diluent quantifying cavity are uniformly mixed in the quantitative uniform mixing cavity, the obtained mixture enters the reaction chamber through the micro-channel for a reaction with a reaction reagent to be detected, and meanwhile, the mixture enters the sample blank chamber to be used as asample blank for detection. The multifunctional micro-fluidic detection chip can effectively reduce sample consumption, improve the accuracy of detection results, and detect multiple indexes at the same time.

The subject invention provides an improved bypass circuit for use in adult and especially pediatric cardiac surgery. The auto regulating capability of this circuit design simplifies its operation and combines the benefits of both VAVD and KAVD systems while eliminating the need for multiple blood pumps. Advantageously, this system occupies less space than existing systems, requires the use of less blood, reduces the contact between blood and tubing, reduces damage to blood cells, eliminates the need for multiple blood pumps and also reduces the incidence of gaseous emboli.

The present invention relates to electrochemical detection technology, and is quick detecting method of aspartate aminotransferase vitality. Electrochemical or optical change is produced corresponding to the vitality of the measured matter. The method includes: A. sucking the measured matter liquid drop into the reaction area between electric poles to generate oxidation-reduction on the surface of the electric poles so as to produce electron transfer and form current; and B. converting the aspartic acid into glutamic acid and oxaloacetic acid under the cooperated action of current and auxiliary factors and measuring the amount of glutamic acid and oxaloacetic acid so as to obtain the corresponding aspartate aminotransferase vitality. The present invention has raised response speed and detection sensitivity of current type enzyme sensor and lowered reaction voltage.

A biosensor for measuring the content of lactic acid in whole blood features that an electrode is covered by plastic film to form a cavity, said plastic film has a capillary hole communicated with said cavity, and the reaction layer consisting of carboxymethyl cellulose, modified carbon nanotubes, and the mixture of L-lactate oxidase, reagent, stabilizer and buffering liquid is put in said cavity. Its advantages are high snesitivity, low voltage (300 mv), and less blood speciment without pretreatment.

The invention discloses a matrix modifying reagent for detecting content of lead and cadmium elements in whole blood, which comprises the following compositions: diammonium phosphate with the mass concentration of 0.5 to 0.6 percent, millesimal polyoxyethylene octyl phenyl ether with volume concentration of 2.0 to 4.0 percent, millesimal concentrated nitric acid with volume concentration of 1.0 to 3.0, and the balance of ultrapure water. The invention also provides a method for detecting content of lead and cadmium elements in the whole blood by the matrix modifying reagent, which dilutes concentration of the whole blood to be between one eighth and one thirteenth of the original concentration by adopting the matrix modifying reagent, and adds the diluted blood to an atomic absorption spectrometer for detecting. The detection method can detect lead and cadmium elements in one time, and has small amount of blood sample, simple operation, and low requirement on environment and operators.

The invention discloses the technical field of fat-soluble vitamin detection and particularly relates to a method for simultaneously detecting multiple fat-soluble vitamins. The detection method comprises the following steps: step 1, fully and uniformly mixing 10-200 microlitres of sample plasma with a protein precipitation solution and a vitamin deuterated internal standard substance at 100-3000rpm for 1-5 min to obtain a solution A, and centrifuging the solution A at 0-8 DEG C at 200-12000 rpm to obtain a supernatant; and step 2, analyzing the fat-soluble vitamin of the supernatant by usinga liquid chromatograph-mass spectrometer, and calculating the content of the fat-soluble vitamin in the sample plasma by using an internal standard method. Compared with the prior art, the method hasthe advantages that more types of vitamins are detected, the sample can be enriched without the assistance of SLE, SPE and other materials, the detection cost is effectively reduced, the required blood volume is less than that of the existing method, the compliance of patients, especially infants, is better due to the reduction of the blood volume of the sample, and the method has a wide application prospect.

The invention discloses a novel closestool. A seat ring of the closestool is in a saddle type; the height difference between the lowest points of the upper end surface and the lower end surface of the seat ring is 55 to 60mm; the length of the seat ring is 280 to 290mm; the width of the seat ring is 350 to 360mm; the length of a seat ring hole formed in the seat ring is 230 to 250mm; the width of the seat ring hole is 170 to 190mm; an included angle between a connecting line between the lowest point of the lower end surface and the highest point of the front end of the upper end surface of the seat ring, and the horizontal tangent line of the lowest point of the lower end surface is 15 to 20 degrees; an included angle between the connecting line between the lowest point of the lower end surface and the highest point of the rear end of the upper end surface of the seat ring, and the horizontal tangent line of the lowest point of the lower end surface is 30 to 45 degrees. The novel closestool has the advantages that in the use process, the lower limbs naturally droop; the skeletal muscle is relaxant; the returned blood volume is reduced; the afterload of the heart is reduced; the elevation-angle-increase sitting posture defecation is realized, so that the defecation body position is comfortable and smooth; the novel closestool conforms to the defecation habit; the defecation time is shortened, so that the constipation occurrence rate is reduced.

The invention relates to a method for detecting contents of folic acid and sulfur-containing amino acid in plasma and belongs to the technical field of analysis and detection. The method comprises the following steps: firstly adding a reducing agent for reducing cysteine and homocysteine in plasma; secondly, adding a precipitant for performing albumen precipitation; thirdly, taking supernatant, and adding a reaction solvent and a derivatization reagent for carrying out amino acid derivatization reaction; and finally, adopting a chromatographic column for performing separation and mass spectrum detection determination. According to the method provided by the invention, the folic acid, cysteine, homocysteine and methionine in plasma can be determined at one time, and the interference of other matters is eliminated. The method has the advantages of high precision, accuracy and sensitivity and wide linearity range and can be applied to detection of the contents of the folic acid, cysteine, homocysteine and methionine in large quantity of plasma samples.

The invention discloses a novel toilet seat cover. A toilet seat of the toilet seat cover is in an inward-recessed saddle shape, when a person sits on the toilet seat, an angle formed between each thigh of the human body and a horizontal plane is 33 to 37 degrees; the front end bottom of the toilet seat is hinged onto the front end of a toilet seat holder, the rear end bottom of the toilet seat is connected with the toilet seat holder by virtue of an electric pushing rod, a pushing rod top end of the electric pushing rod is hinged to the rear end bottom of the toilet seat, and the electric pushing rod is fixed on the toilet seat holder; and the rear end top of the toilet seat is hinged to a toilet top cover. When the toilet seat cover is used, lower limbs of the person droop naturally, skeletal muscles are relaxed, the returned blood volume is reduced, the after load of heart is alleviated, by increasing the elevation, the defecation position is comfortable, the defecation is smooth, the defecation habit can be satisfied, the defecation time can be shortened, and the constipation rate is reduced. In addition, by arranging the electric pushing rod, the toilet seat has an automatic bouncing function, after the defecation, as long as a control switch is pressed, the toilet seat can be slowly bounced for 45 degrees, and the senior can stand up easily.

The invention relates to a method for in-vitro culture of killer T cells. Specifically, IL-2 and an anti-CD3 monoclonal antibody are combined with indigowoad leaf decoction for costimulation of lymphocytes taken from 50ml of in-vitro blood so as to get the T cells with cytotoxin activity by induction; and the T cells with the cytotoxin activity, which are obtained by an induction culture method, have the advantages of large proliferation number and good vitality, and the number of the CD8-positive T cells can be increased from 20%-30% to 80%-90%. Simultaneously, the method disclosed by the invention has the advantages of low taking quantity of the blood, simple operation steps, low cost of a reagent and short maturation time of the cells, above 1*10<9> cells can be achieved by 8 days and used for performing continuous intravenous infusion on a patient six times, and above 1*10<9> cells can be infused every time. The method is suitable for a clinical biological immunotherapy of the patients with tumors and can be widely popularized and applied.

A method of relieving pain by manipulation of those parts of a body in order to direct pressurized blood to be brought into contact with ischemic nerves by intraneural facilitation comprising the steps of pressurizing blood vessels proximate to the pain source while performing one or more of the following: stretching the muscles, mobilizing the joints, tractioning the skin, distending the viscera, and distorting the vascular system.

The invention relates to a method for jointly detecting a lung cancer tumor marker based on flow cytometry. The method comprises the steps: step one, preparing a probe I; to be specific, preparing different types of fluorescent coded microspheres, marking the fluorescent coded microspheres with streptavidin, marking a capture antibody with a biotin, and carrying out coupling between the coded microspheres marked by streptavidin and the capture antibody marked by the biotin; step two, preparing a probe II; to be specific, marking a detection antibody with fluorescent dye; step three, carrying out multi-component biomarker detection by a fluorescence resonance energy transfer system in a reaction system; to be specific, in the reaction system, binding the probe I with the the probe II to form a fluorescence resonance energy transfer system under the condition of existing a tumor marker in an added sample, introducing the detected fluorescence intensities of the coded microspheres at the maximum emission wavelength of the probe II into a work curve to obtain concentrations of different types of tumor markers. The method has advantages of high sensitivity, fast implementation, high throughput, and low price.

The invention provides a method and a kit for simultaneously detecting multiple steroid hormones in a blood sample. According to the method, a biological sample is pretreated through methanol deproteinization combination of sodium bicarbonate and a solid-phase support extraction method, chromatographic separation and mass spectrometric detection are performed, a pair of qualitative ions and a pairof quantitative ions are selected for each hormone, and the relative retention time and the qualitative ion pair are used as qualitative basis to determine the content of the sodium bicarbonate in the biological sample. And making a standard curve for quantification by using a standard substance. Meanwhile, three levels of quality control products are applied to investigate the accuracy and effectiveness of the method, and detection result distortion is avoided. The purpose of simultaneously detecting eighteen hormones in a serum sample by applying an HPLC-MS / MS technology is achieved for thefirst time, the influence of interferents is reduced, the method is easy, convenient and rapid to operate, high in flux and low in cost, the steroid hormone level in a human body is effectively monitored in real time, a sufficient basis is provided for clinical disease diagnosis, and the method is worthy of popularization and application.

The invention provides a method for determining folic acids in blood spots through high performance liquid chromatography-tandem mass spectrometry. The folic acid and 5-methyltetrahydrofolate are separated by high performance liquid chromatography, and then mass spectrometry isotope internal standard quantitation is used. The concentration of the standard is taken as the X-axis, and the peak arearatio of the standard to the internal standard is the Y-axis to establish a calibration curve. The content of the folic acid and 5-methyltetrahydrofolate is calculated. The method provided by the invention has the advantages of high sensitivity, high specificity, accuracy, simple pretreatment process, short time and greatly saved time cost.

The present invention relates to a drug for treating dysfunctional uterine bleeding, the India madder root, Sanguis Draconis and carbonized human hair are mixed and smashed into the fine powder, the Chinese angelica, baical skullcap root, trogopterus dung, cattail pollen, south dodder seed etc. are boiled by water and then filtered and condensed into the thick paste, the drug powder is added for even mixing, finally the product is prepared by smashing and filling in the capsule. The drug of the present invention has the effects of nourishing blood, cooling blood, activating blood circulation, removing blood stasis and hemostasis, which is applicable to the treatment of the advanced menstruation of women, large amount of menstrual bleeding or large amount of uterine bleeding caused by contraceptive ring embedding and drug abortion and other diseases; the present invention has the advantages of easy administration, accurate efficacy and no toxicity or side effects etc. The total effective rate can reach 96.0 percent through the clinical efficacy observations of 100 cases of patients with dysfunctional uterine bleeding.

The invention discloses a microfluidic blood type detection chip. The chip comprises a chip body, and the chip body comprises a positive type blood type identification area which is provided with a first sample injection cavity, a quantitative uniform mixing cavity, a positive type reaction cavity and an air hole; a reverse typing blood type identification area which is provided with a second sample injection cavity, a quantitative separation cavity, a reverse typing reaction cavity and an air hole; a sample is injected into the first sample injection cavity, and the sample and a preset diluent in the first sample injection cavity enter the quantitative uniform mixing cavity through the micro-channel; a sample and a diluent are uniformly mixed in the quantitative uniform mixing cavity and then enter the positive setting reaction cavity to react with a reaction reagent for detection; a sample is injected into the second sample injection cavity and enters the quantitative separation cavity through the micro-channel, the sample is separated in the quantitative separation cavity, and separated plasma enters the reverse typing reaction cavity to react with a reaction reagent for detection. The positive and negative typing blood type identification can be simultaneously carried out, so that the blood type identification result is more accurate.

The invention relates to a method for simultaneously detecting homocysteine and metabolism-related substances thereof. The method comprises the following steps: pre-treating a blood sample, analyzing and detecting by adopting liquid chromatography-tandem mass spectrometry, and finally quantifying by adopting an internal standard method. The method can be used for simultaneously detecting the contents of homocysteine and metabolism-related substances (methionine, pyridoxine, folic acid and 5-methyltetrahydrofolic acid) in human serum, and carrying out accurate qualitative and quantitative analysis, and is a detection method which is simple in sample treatment, high in flux and reliable in result.

The subject invention provides an improved bypass circuit for use in adult and especially pediatric cardiac surgery. The auto regulating capability of this circuit design simplifies its operation and combines the benefits of both VAVD and KAVD systems while eliminating the need for multiple blood pumps. Advantageously, this system occupies less space than existing systems, requires the use of less blood, reduces the contact between blood and tubing, reduces damage to blood cells, eliminates the need for multiple blood pumps and also reduces the incidence of gaseous emboli.

The invention relates to a method and kit for preparing a library of free miRNAs in blood, and the method mainly comprises the following steps: (a) separating serum or plasma from blood, and extracting free RNAs; (b) mixing the free RNA with exogenous RNA, wherein any part of an exogenous RNA sequence is not overlapped with a known miRNA sequence of a human / rat / mouse; (c) adding a linker to the RNA mixture in the step (b), carrying out reverse transcription on the first strand and the second strand for synthesis and amplification, and recovering the target DNA fragment to obtain the free miRNAsequencing library. The invention further relates to a miRNA expression quantification method. The method comprises the steps (a), (b) and (c), the miRNA sequencing library is subjected to next-generation sequencing and data analysis, in the data analysis, the S2 random tag sequence of the adaptor RA5 can be used as a quantification tag, the PCR repetitive sequence is removed, and the detection accuracy is improved.

The invention discloses a stretching and pushing massage treatment apparatus for rheumatic diseases. For the stretching and pushing massage treatment apparatus for rheumatic diseases, connecting rodsare arranged on fixing stand columns vertically arranged on the periphery of a main bracket, connecting rings are arranged at the vertex angle positions or the middle of one arch-shaped elastic plate,and the connecting rings are hinged to the end parts of the corresponding connecting rods; pushing and pressing rods are vertically fixed to the lower sides of an arch-shaped spring, and a smooth part is arranged at the tail end of each pushing and pressing rod; a drive mechanism is fixed in the middle of the main bracket, an eccentric slider is also mounted in the middle of the main bracket, anda fixing belt is connected to the lower side of each fixing stand column. According to the massaging mode adopted by the invention, pressure is applied towards two sides from the center and is pushedtowards the outside, during the pushing process, the blood vessels and the tissue in the middle position of the massage area are extruded, so that the blood amount is reduced, during the non-processing process in the cycle period, the tissue cells and the blood vessels start relaxation for hyperaemia, and after repeated stretching and pushing as well as pressing, the effect equivalent to the stretching and pushing as well as pressing effect of the hands is achieved.

The invention relates to a gynecological traditional Chinese medicine, and in particular relates to a traditional Chinese medicine preparation for treating primary vaginal dripping. The traditional Chinese medicine preparation is prepared from ginseng, bighead atractylodes rhizome, tuckahoe, radix rehmanniae carbo, fried white peony root, ass-hide glue, moxa carbo, ginger carbo, cuttlebone, dodder, eucommia, teasel root and bitter cardamon as raw materials according to certain proportions by weight, and can be processed into pills, tablets or capsules. The traditional Chinese medicine preparation provided by the invention has an obvious effect on treating symptoms of vaginal dripping, pale menstrual flow and dripping menorrhea by nourishing the kidney and yang and enriching the blood for convergence, is simple to take, and is safe and reliable in clinical application.