33results about How to "Influence binding" patented technology

The invention discloses a quick trenbolone residue detection test paper card and a preparation method thereof. According to the technical scheme, the quick trenbolone residue detection test paper card is characterized by comprising a plastic box body and a test strip encapsulated in the plastic box body, wherein a supporting layer is arranged on the bottom layer of the test strip, and a sample pad, a gold-labeled antibody bonding pad, a nitrocellulose membrane and an absorption pad which are closely connected with one another are sequentially attached to the supporting layer from left to right; a detection line printed by a trenbolone-albumin egg conjugate solution and a quality control line printed by a goat anti mouse IgG solution are arranged on the nitrocellulose membrane; and a colloidal-gold-labeled anti-trenbolone specific mono-clonal antibody is filled in the gold-labeled antibody bonding pad. The invention also discloses the preparation method of the quick trenbolone residue detection test paper card. The quick trenbolone residue detection test paper card has the advantages of simple operation, quickness, accuracy, high sensitivity, strong specificity, low cost and high stability, and can be used for batch detection.

The invention relates to a side-view type LED packaging structure as well as a production method and application thereof. The side-view type LED packaging structure comprises a silicon substrate, a first conducting pin, a second conducting pin and a first LED chip. The silicon substrate comprises a groove which defines the exit surface of the side-view type LED packaging structure; the first conducting pin is positioned on at least part of the groove and extends to one outer surface of the silicon substrate; the second conducting pin is positioned on at least part of the groove and extends to the outer surface of the silicon substrate, and the first conducting pin and the second conducting pin are separated electrically; the first LED chip comprises a first electrode and a second electrode which are respectively electrically connected with the first conducting pin and the second conducting pin in the groove; and the outer surface of the silicon substrate is perpendicular to the exit surface.

The present invention provides method of raising binding force of chemical coating on the surface of NdFeB permanent magnet. The NdFeB permanent magnet is pre-coated in alkaline plating solution under ultrasonic action, and by means of the activation, cavitation and stirring of ultrasonic wave, one homogeneous and compact initial deposited layer is formed on the surface of the magnet, so as to raise the binding force of chemical nickel-base coating on the surface of the magnet obviously and reduce pores in the coating. The present invention also provides the technological process including deoiling and pickling to activate before plating, the ultrasonic pre-plating and the conventional chemical plating. The formed coating has high corrosion resistance, raised binding to the substrate and perfect protection on the NdFeB permanent magnet.

The invention relates to a divisional application with the application number of 202010060055.7. The invention relates to a lectin-magnetic carrier coupling compound for separating glycosylated exosomes in a clinical sample, and the lectin-magnetic carrier coupling compound comprises a magnetic carrier, a binding agent, a binding agent and a binding agent, and lectin coupled to the outside of the magnetic carrier. The lectin-magnetic carrier coupling compound provided by the invention can be used for rapidly, accurately and automatically separating the glycosylated exosome in a clinical sample, the separation efficiency is high, and the separated exosome is complete in form and free from rupture or fragmentation, and can be directly used for glycosylated exosome liquid detection or directly used for related immunology detection, so that the detection efficiency is high. Or directly extracting related nucleic acid in the exosome for nucleotide sequence detection and analysis.

The invention relates to a ceramic matrix composite heat-resistant board and a preparation method thereof. (1) Prepare a fiber preform according to design; (2) Prepare an interface layer; (3) Repeat dipping-curing-cracking 2-6 times; (4) ) Process the heat-resistant plate product through holes according to the design size, and process the head of the bolt and the external thread of the screw according to the design size; (5) Apply the ceramic precursor slurry containing nanofibers on the surface of the bolt and the through hole, The bolts pass through the through holes of the heat-resistant plate; (6) Repeat the dipping-curing-low temperature cracking 2-4 times, and then repeat the dipping-curing-high temperature cracking 1-2 times; (7) CVI technology densifies the SiC matrix; (8) ) CVD deposition of SiC coating to obtain ceramic matrix composite heat-resistant plate. The heat-resistant plate produced by the invention is resistant to high temperature, can effectively prevent the engine tail flame from entering the back of the heat-resistant plate from the gap to ablate the metal bracket, prolong the service life of the metal bracket and reduce the use cost.

The invention provides a lomefloxacin immunogen which has the structure represented by the formula I. A preparation method of the lomefloxacin immunogen is provided and comprises the steps of carrying out C chain derivatization of carboxyl on lomefloxacin, and activating BSA by using a glycosylation modification method, then synthesizing and immunizing by using a carbodiimide two-step method, and thus obtaining the immunogen having the characteristics of high coupling rate and strong immunity. The invention also provides a test paper card for detecting the lomefloxacin, wherein the test paper card has the advantages of fast detection speed and high sensitivity; and the detection limit of the test paper card can be reach 0.3 ng / ml, and the requirement of rapid detection can be met.

The invention relates to a side-view type LED packaging structure as well as a production method and application thereof. The side-view type LED packaging structure comprises a silicon substrate, a first conducting pin, a second conducting pin and a first LED chip. The silicon substrate comprises a groove which defines the exit surface of the side-view type LED packaging structure; the first conducting pin is positioned on at least part of the groove and extends to one outer surface of the silicon substrate; the second conducting pin is positioned on at least part of the groove and extends to the outer surface of the silicon substrate, and the first conducting pin and the second conducting pin are separated electrically; the first LED chip comprises a first electrode and a second electrode which are respectively electrically connected with the first conducting pin and the second conducting pin in the groove; and the outer surface of the silicon substrate is perpendicular to the exit surface.

The invention relates to a mandibular gingival flap stretching fixation clamp which has simple structure, is convenient to use and is used in gingival flap implant operation. When in use, a left alveolar bone fixing arm and a right alveolar bone fixing arm clamp the alveolar bone from the left and right sides, a left gingival flap stretching arm and a right gingival flap stretching arm can move oppositely, gingival flap stretching plates on the left and right gingival flap stretching arms stretch the gingival flap to the left and right sides so that the surgical site on the top of the alveolar bone can be fully exposed for operation, the dental drill for holing and the implant can not contact the gingival soft tissue, and the soft tissue can not be carried into the cavity to affect synosteosis; and after the gingival flap is stretched by the stretching fixation clamp, the hands of the doctor can be free and the operation can be performed smoothly. The mandibular gingival flap stretching fixation clamp is mainly used to stretch and fix the gingival flap after gingival is flapped in the mandibular teeth implant operation and can also be used in the mandibular teeth alveolar ridge trimming and forming operation.

The invention relates to a DNA pulldown method and a kit. The method includes the following steps of pretreating magnetic beads, wherein the magnetic beads marked by BSA(bovine serum albumin) and oligonucleotide random primer closed streptavidin are used; preparing a probe-magnetic bead compound; extracting and pretreating cell nucleus protein, wherein deoxyribonuclease is added into an extracting nucleoprotein sample for incubation, the magnetic beads are added for incubation and then removed, and supernatant is collected; carrying out pulldown; collecting the protein sample. The DNA pulldown method has the following advantages that BSA and an oligonucleotide random primer are used for closing the magnetic beads in advance, and nonspecific binding between the magnetic beads, protein and genomic DNA is reduced. Nuclease contamination is prevented through systemic nuclease protection, stability and reliability of experiments are improved, repeatability is high, and the experiment process is simplified.

The invention relates to environment-friendly metal composite plate production equipment which comprises a body. A hot rolling chamber, a treatment mechanism and two grinding chambers are arranged inthe body. Two extrusion devices are arranged in the hot rolling chamber. A grinding mechanism is arranged in each grinding chamber. Each grinding mechanism comprises a first motor, a movement unit anda transmission unit. The treatment mechanism comprises an air extraction unit, a heat exchange unit and a purification unit. The air extraction unit comprises a drive assembly, a push plate, a compression pipe, a gas inlet pipe and an exhaust pipe. According to the environment-friendly metal composite plate production equipment, through the grinding mechanisms, the metal composite plate production equipment can remove oxide films on the surface of a metal raw material plate, and bonding between two kinds of metal is prevented from being influenced by oxide; and moreover, through the treatmentmechanism, the metal composite plate production equipment can treat and purify harmful gas released by the metal in the high-temperature and high-pressure environment, and the harmful gas is prevented from corroding parts in the metal composite plate production equipment.

The invention discloses a quick trenbolone residue detection test paper card and a preparation method thereof. According to the technical scheme, the quick trenbolone residue detection test paper card is characterized by comprising a plastic box body and a test strip encapsulated in the plastic box body, wherein a supporting layer is arranged on the bottom layer of the test strip, and a sample pad, a gold-labeled antibody bonding pad, a nitrocellulose membrane and an absorption pad which are closely connected with one another are sequentially attached to the supporting layer from left to right; a detection line printed by a trenbolone-albumin egg conjugate solution and a quality control line printed by a goat anti mouse IgG solution are arranged on the nitrocellulose membrane; and a colloidal-gold-labeled anti-trenbolone specific mono-clonal antibody is filled in the gold-labeled antibody bonding pad. The invention also discloses the preparation method of the quick trenbolone residue detection test paper card. The quick trenbolone residue detection test paper card has the advantages of simple operation, quickness, accuracy, high sensitivity, strong specificity, low cost and high stability, and can be used for batch detection.

The invention discloses a test paper card for quickly detecting residues of fluoroquinolones and a preparation method of the test paper card. The key points of the technical scheme of the test paper card are as follows: the test paper card for quickly detecting the residues of the fluoroquinolones comprises a plastic box body and a test strip which is packaged in the plastic box body, wherein the bottom layer of the test strip is used as a supporting layer; a sample pad, a gold-labeled antibody combination pad, a cellulose nitrate film and an absorption pad which are closely connected with one another are adhered on the supporting layer from left to right in sequence; a detection line which is printed by using a norfloxacin and edge albumin conjugate solution and a quality control line which is printed by using a goat anti-rat IgG solution are arranged on the cellulose nitrate film; and colloidal-gold-labeled anti-fluoroquinolone-type specific monoclonal antibodies are poured on the combination pad. The invention also discloses a method for preparing the test paper card for quickly detecting the residues of the fluoroquinolones. The test paper card is easy to operate, fast, accurate, high in sensitivity, low in cost and high in stability, and can be used for performing batch detection.

The invention discloses a leather degreasing agent, a preparation method and application thereof. A degreasing agent comprising one, two, three or more isomeric alcohol block polyethers selected from the following formula (I): RO[CH 2 CH(CH 3 )O] n (CH 2 CH 2 O) m H (I); Wherein, R is selected from the isomeric alkyl group with an average degree of isomerization ≥ 2; n is selected from the number of 1-8; m is selected from the number of 0.5-15. The degreasing agent has good penetration and emulsifying effect, which can effectively reduce the oil content in the leather embryo; the fatliquoring agent is suitable for the processing and degreasing of cowhide, sheepskin, pigskin and other leather products.

The invention relates to an RNA pulldown method and a kit. The method includes the following steps of S1, pretreating magnetic beads by means of using BSA and a random oligonucleotide primer to enclose magnetic beads containing streptavidin; S2, preparing probe-magnetic bead complex; S3, extracting and pretreating whole cell proteins by means of adding ribonuclease into whole cell protein extraction samples for incubation, then adding the magnetic beads for incubation, and collecting supernatant; S4, performing pulldown; S5, collecting RNP. The method and the kit have the advantages that the BSA and the random oligonucleotide primer are used for enclosing the magnetic beads in advance, so that nonspecific binding of the magnetic beads with the proteins and RNA is lowered. Through systematic nuclease protection, pollution of nuclease is prevented, stability and reliability in tests are improved, repeatability is good, and test procedures are simplified.

The invention discloses a beta-diketone organotin PVC heat stabilizer and a preparation method thereof, and belongs to the technical field of a PVC heat stabilizer. According to main points of the technical scheme, the beta-diketone organotin PVC heat stabilizer has a structural formula as shown in the specification, wherein R is phenyl or heptadecyl. The invention also discloses a preparation method of the beta-diketone organo tin PVC heat stabilizer. Advantages of an organotin stabilizing agent and a beta-diketone compound auxiliary heat stabilizer are combined, thus achieving the effects of the two substances. Then, the product is more convenient to use. In the preparation method of the beta-diketone organotin PVC heat stabilizer, the use of ultrasonic wave can avoid ammonium chloride which is generated after a reaction and doped in the product from affecting quality of the product; and by the use of nitrogen during the whole reaction, a solvent is not easy to volatilize under the condition of 0 DEG C. through nitrogen for solvent volatilization, crystallization can be promoted, and the influence of oxygen on the combination of a beta-diketone compound and stannous ion can be avoided.