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33results about How to "Influence binding" patented technology

Method of raising binding force of chemical coating on the surface of NdFeB permanent magnet

The present invention provides method of raising binding force of chemical coating on the surface of NdFeB permanent magnet. The NdFeB permanent magnet is pre-coated in alkaline plating solution under ultrasonic action, and by means of the activation, cavitation and stirring of ultrasonic wave, one homogeneous and compact initial deposited layer is formed on the surface of the magnet, so as to raise the binding force of chemical nickel-base coating on the surface of the magnet obviously and reduce pores in the coating. The present invention also provides the technological process including deoiling and pickling to activate before plating, the ultrasonic pre-plating and the conventional chemical plating. The formed coating has high corrosion resistance, raised binding to the substrate and perfect protection on the NdFeB permanent magnet.
Owner:ZHEJIANG UNIV

Quick trenbolone residue detection test paper card and preparation method thereof

The invention discloses a quick trenbolone residue detection test paper card and a preparation method thereof. According to the technical scheme, the quick trenbolone residue detection test paper card is characterized by comprising a plastic box body and a test strip encapsulated in the plastic box body, wherein a supporting layer is arranged on the bottom layer of the test strip, and a sample pad, a gold-labeled antibody bonding pad, a nitrocellulose membrane and an absorption pad which are closely connected with one another are sequentially attached to the supporting layer from left to right; a detection line printed by a trenbolone-albumin egg conjugate solution and a quality control line printed by a goat anti mouse IgG solution are arranged on the nitrocellulose membrane; and a colloidal-gold-labeled anti-trenbolone specific mono-clonal antibody is filled in the gold-labeled antibody bonding pad. The invention also discloses the preparation method of the quick trenbolone residue detection test paper card. The quick trenbolone residue detection test paper card has the advantages of simple operation, quickness, accuracy, high sensitivity, strong specificity, low cost and high stability, and can be used for batch detection.
Owner:HENAN INST OF SCI & TECH

DNA pulldown method and kit

The invention relates to a DNA pulldown method and a kit. The method includes the following steps of pretreating magnetic beads, wherein the magnetic beads marked by BSA(bovine serum albumin) and oligonucleotide random primer closed streptavidin are used; preparing a probe-magnetic bead compound; extracting and pretreating cell nucleus protein, wherein deoxyribonuclease is added into an extracting nucleoprotein sample for incubation, the magnetic beads are added for incubation and then removed, and supernatant is collected; carrying out pulldown; collecting the protein sample. The DNA pulldown method has the following advantages that BSA and an oligonucleotide random primer are used for closing the magnetic beads in advance, and nonspecific binding between the magnetic beads, protein and genomic DNA is reduced. Nuclease contamination is prevented through systemic nuclease protection, stability and reliability of experiments are improved, repeatability is high, and the experiment process is simplified.
Owner:GUANGZHOU BIOSENSE BIOSCI

Beta-diketone organotin PVC heat stabilizer and preparation method thereof

The invention discloses a beta-diketone organotin PVC heat stabilizer and a preparation method thereof, and belongs to the technical field of a PVC heat stabilizer. According to main points of the technical scheme, the beta-diketone organotin PVC heat stabilizer has a structural formula as shown in the specification, wherein R is phenyl or heptadecyl. The invention also discloses a preparation method of the beta-diketone organo tin PVC heat stabilizer. Advantages of an organotin stabilizing agent and a beta-diketone compound auxiliary heat stabilizer are combined, thus achieving the effects of the two substances. Then, the product is more convenient to use. In the preparation method of the beta-diketone organotin PVC heat stabilizer, the use of ultrasonic wave can avoid ammonium chloride which is generated after a reaction and doped in the product from affecting quality of the product; and by the use of nitrogen during the whole reaction, a solvent is not easy to volatilize under the condition of 0 DEG C. through nitrogen for solvent volatilization, crystallization can be promoted, and the influence of oxygen on the combination of a beta-diketone compound and stannous ion can be avoided.
Owner:南通德发生物化工有限公司

Environment-friendly metal composite plate production equipment

The invention relates to environment-friendly metal composite plate production equipment which comprises a body. A hot rolling chamber, a treatment mechanism and two grinding chambers are arranged inthe body. Two extrusion devices are arranged in the hot rolling chamber. A grinding mechanism is arranged in each grinding chamber. Each grinding mechanism comprises a first motor, a movement unit anda transmission unit. The treatment mechanism comprises an air extraction unit, a heat exchange unit and a purification unit. The air extraction unit comprises a drive assembly, a push plate, a compression pipe, a gas inlet pipe and an exhaust pipe. According to the environment-friendly metal composite plate production equipment, through the grinding mechanisms, the metal composite plate production equipment can remove oxide films on the surface of a metal raw material plate, and bonding between two kinds of metal is prevented from being influenced by oxide; and moreover, through the treatmentmechanism, the metal composite plate production equipment can treat and purify harmful gas released by the metal in the high-temperature and high-pressure environment, and the harmful gas is prevented from corroding parts in the metal composite plate production equipment.
Owner:安徽荣宸汽车零部件制造有限公司

Beta-diketone organic barium-zinc PVC heat stabilizer and preparation method thereof

The invention discloses a beta-diketone organic barium-zinc PVC heat stabilizer and a preparation method thereof, and belongs to the technical field of PVC heat stabilizers. According to the technical scheme, the beta-diketone organic barium-zinc PVC heat stabilizer is prepared through the steps that a beta-diketone organic barium compound and a beta-diketone organic zinc compound are added into dichloromethane, and after dissolution by heating is conducted, crystallization by cooling is conducted, wherein the structural formula of the beta-diketone organic barium compound is shown as follows: (please see the formula in the description), the structural formula of the beta-diketone organic zinc compound is shown as follows: (please see the formula in the description), and R represents a phenyl group. The invention further discloses the preparation method of the beta-diketone organic barium-zinc PVC heat stabilizer. The beta-diketone organic barium-zinc PVC heat stabilizer integrates the advantages of a barium stabilizer and a zinc stabilizer and the advantages of a beta-diketone compound auxiliary heat stabilizer, achieves the effect of simultaneously using the two substances and is more convenient to use.
Owner:吉林晟康医药包装有限公司

Ofloxacin-hemocyanin coating antigen and preparation method thereof and detection test card

The invention provides an ofloxacin-hemocyanin coating antigen and a preparation method thereof. The structure of the ofloxacin-hemocyanin coating antigen is shown in the description. The preparation method of the ofloxacin-hemocyanin coating antigen includes the steps that C-chain derivatization is conducted on carboxyl of ofloxacin, activation is conducted on KLH at the same time, an acid-anhydride coupling method is adopted to synthesize the coating antigen, and the prepared coating antigen has the advantages of high coupling efficiency and reactogenicity. The invention further provides a test card for testing the ofloxacin, the test card is fast in detection and high in specificity and sensitivity, the detection limit of the test card can reach 0.06 ng / ml, and the requirements of rapid detection are met at the same time.
Owner:HENAN INST OF SCI & TECH

Pre-plating process of chemically-plated Ni-P alloy

The invention relates to a pre-plating process of a chemically-plated Ni-P alloy, in particular to an ultrasonic pre-plating process of the chemically-plated Ni-P alloy. Ultrasonic pre-plating frequency is between 40 and 50 kHz, ultrasonic pre-plating power is 100W+ / -10, ultrasonic pre-plating temperature is between 56 and 60 DEG C and ultrasonic pre-plating time is between 20 and 30 minutes. A pre-plated layer obtained by the process is uniform and dense and is tightly bonded with a substrate, and the bonding force between the substrate and the plated layer can be enhanced remarkably.
Owner:SANVAC BEIJING MAGNETICS +1

Side-view type LED packaging structure as well as production method and application thereof

The invention relates to a side-view type LED packaging structure as well as a production method and application thereof. The side-view type LED packaging structure comprises a silicon substrate, a first conducting pin, a second conducting pin and a first LED chip. The silicon substrate comprises a groove which defines the exit surface of the side-view type LED packaging structure; the first conducting pin is positioned on at least part of the groove and extends to one outer surface of the silicon substrate; the second conducting pin is positioned on at least part of the groove and extends to the outer surface of the silicon substrate, and the first conducting pin and the second conducting pin are separated electrically; the first LED chip comprises a first electrode and a second electrode which are respectively electrically connected with the first conducting pin and the second conducting pin in the groove; and the outer surface of the silicon substrate is perpendicular to the exit surface.
Owner:CHI MEI LIGHTING TECH

Hub with automatic pressure adjusting pack

A hub with an automatic pressure adjusting pack is characterized in that a rim cavity 7 is formed in a rim of a hub body 0; the pressure adjusting pack 1 is arranged in the rim cavity 7; an air nipple 6 is arranged on the pressure adjusting pack 1 and provided with an air nipple sealing pad 4 and an air nipple cap nut 5; a tension spring 2 is arranged inside the pressure adjusting pack 1; positioning skin 3 is arranged at the two ends of the tension spring 2; a rim cavity opening 8 is formed in the outer side of the rim cavity 7; a rim cavity insertion opening 9 is arranged on the upper portion of the rim cavity opening 8; a rim cavity screw hole 10 is formed in the lower portion of the rim cavity opening 8; the rim cavity opening 8 is provided with a rim cavity cover 11; the rim cavity cover 11 is provided with a cavity cover screw 12. The pressure adjusting pack with the tension spring with the setting tension is used for buffering air when the tire pressure is increased, and the tire air pressure is relieved; when the air temperature is decreased and the tire air pressure is decreased, the pressure adjusting pack release the buffered air under the action of the tension spring and corrects unstable pressure, automatic tire pressure adjustment is ensured, tire puncturing is prevented, and a vehicle runs more safely in a more oil-saving mode.
Owner:WUXI XIYANGKANG SCI & TECH

Test paper card for rapidly detecting vomitoxin

The invention provides a test paper card for rapidly detecting vomitoxin. The test paper card comprises a plastic box and a test strip packaged in the plastic box. The test strip comprises a bottom support layer, a sample pad, a gold label and antibody binding pad, a nitrocellulose membrane and an adsorption pad. The sample pad, the gold label and antibody binding pad, the nitrocellulose membrane and the adsorption pad are orderly fixed on the bottom support layer. The nitrocellulose membrane is provided with a T line and a C line. The colloidal gold-labeled anti-vomitoxin monoclonal antibody is poured into the gold label and antibody binding pad. The plastic box is provided with a sample filling hole and an observation window. The position of the sample filling hole is corresponding to the position of the sample pad on the test strip. The position of the observation window is corresponding to the position of the nitrocellulose membrane on the test strip. The test paper card has high sensitivity and strong specificity.
Owner:HENAN ZHIWEI BIOLOGICAL ENG

Probe for capturing RNAm<1>A modified binding protein and method thereof

The invention discloses a probe for capturing RNAm<1>A modified binding protein and a method thereof. The probe is composed of a base stem-loop structure, the length of the probe is 32-632 base, and 3'or 5 'of the probe is provided with a biotin label, and the probe can be bound with avidin on a magnetic bead. The probe is coupled to the magnetic beads by desulfurized biotin labelling and streptavidin and is in affinity binding with desulfurized biotin. The cell extraction total protein is incubated with a magnetic bead-probe compound and the acting protein can be specifically bound with an RNAm<1>A modified probe; the non-specific binding protein can be removed through washing; and finally, eluting is performed with an eluent to obtain a probe-protein compound. According to the specific RNAm<1>A modified protein and a secondary structure, the RNAm<1>A modified binding protein can be captured effectively and the enrichment of the specific binding protein is remarkably improved.
Owner:SHANGHAI FIRST MATERNITY & INFANT HOSPITAL

Hot galvanizing plastic dipping coating method

The invention provides a hot galvanizing plastic dipping coating method, and belongs to the technical field of galvanizing coating. The hot galvanizing plastic dipping coating method comprises the following steps that a silane coupling agent, water and alcohol are mixed, the pH value of an obtained mixed solution is adjusted to 4-5, and then hydrolyzing is conducted to obtain a silane coupling agent hydrolysis solution; the silane coupling agent hydrolysis solution is sprayed on the surface of a hot galvanized workpiece, then drying and preheating are conducted, and a silane film is formed on the surface of the hot galvanized workpiece to obtain a dried and preheated part; the dried and preheated part is soaked in polyethylene powder, and a plastic dipping part is obtained; and the plastic dipping part is cured. According to the hot galvanizing plastic dipping coating method, the silane coupling agent hydrolysis solution is utilized to form bonding between hot galvanizing and polyethylene interfaces, so that the binding force between the hot galvanizing and polyethylene is improved.
Owner:河北中科同创科技发展有限公司

A kind of ceramic matrix composite heat-resistant plate and preparation method thereof

The invention relates to a ceramic matrix composite heat-resistant board and a preparation method thereof. (1) Prepare a fiber preform according to design; (2) Prepare an interface layer; (3) Repeat dipping-curing-cracking 2-6 times; (4) ) Process the heat-resistant plate product through holes according to the design size, and process the head of the bolt and the external thread of the screw according to the design size; (5) Apply the ceramic precursor slurry containing nanofibers on the surface of the bolt and the through hole, The bolts pass through the through holes of the heat-resistant plate; (6) Repeat the dipping-curing-low temperature cracking 2-4 times, and then repeat the dipping-curing-high temperature cracking 1-2 times; (7) CVI technology densifies the SiC matrix; (8) ) CVD deposition of SiC coating to obtain ceramic matrix composite heat-resistant plate. The heat-resistant plate produced by the invention is resistant to high temperature, can effectively prevent the engine tail flame from entering the back of the heat-resistant plate from the gap to ablate the metal bracket, prolong the service life of the metal bracket and reduce the use cost.
Owner:GONG YI VAN-RES INNOVATION COMPOSITE MATERIAL CO LTD

Acridine compound-labeled steroid hormone derivative as well as preparation method and application thereof

The invention relates to the field of biological detection, and particularly provides an acridine compound-labeled steroid hormone derivative as well as a preparation method and application thereof. An acridine compound provided by the invention is used for labeling a steroid hormone derivative, and the acridine compound and the steroid hormone derivative are connected by taking modified polyethylene glycol as a bridging substance. The acridine compound-labeled steroid hormone derivative is good in luminescence property, high in detection sensitivity, low in false positive rate, high in product yield and stable in performance.
Owner:GUANGDONG FAPON BIOTECH CO LTD

A kind of dna pulldown method and kit

The invention relates to a DNA pulldown method and a kit. The method includes the following steps of pretreating magnetic beads, wherein the magnetic beads marked by BSA(bovine serum albumin) and oligonucleotide random primer closed streptavidin are used; preparing a probe-magnetic bead compound; extracting and pretreating cell nucleus protein, wherein deoxyribonuclease is added into an extracting nucleoprotein sample for incubation, the magnetic beads are added for incubation and then removed, and supernatant is collected; carrying out pulldown; collecting the protein sample. The DNA pulldown method has the following advantages that BSA and an oligonucleotide random primer are used for closing the magnetic beads in advance, and nonspecific binding between the magnetic beads, protein and genomic DNA is reduced. Nuclease contamination is prevented through systemic nuclease protection, stability and reliability of experiments are improved, repeatability is high, and the experiment process is simplified.
Owner:GUANGZHOU BIOSENSE BIOSCI

Device and method for producing workpieces with surfaces consisting of rust-resistant metal

The invention relates to a method for producing a workpiece with a surface made of a rust-resistant material. According to the invention, a workpiece 4 of unalloyed steel is cast and subsequently immersed in a melt 2 of a liquid rust-resistant material and covered with a layer of rust-resistant material. The invention also relates to a device comprising a crucible 1 and a lifting mechanism 3 and for carrying out the method according to the invention.
Owner:ANDRITZ HYDRO GMBH

Environmentally friendly metal composite panel production equipment

The invention relates to environment-friendly metal composite plate production equipment which comprises a body. A hot rolling chamber, a treatment mechanism and two grinding chambers are arranged inthe body. Two extrusion devices are arranged in the hot rolling chamber. A grinding mechanism is arranged in each grinding chamber. Each grinding mechanism comprises a first motor, a movement unit anda transmission unit. The treatment mechanism comprises an air extraction unit, a heat exchange unit and a purification unit. The air extraction unit comprises a drive assembly, a push plate, a compression pipe, a gas inlet pipe and an exhaust pipe. According to the environment-friendly metal composite plate production equipment, through the grinding mechanisms, the metal composite plate production equipment can remove oxide films on the surface of a metal raw material plate, and bonding between two kinds of metal is prevented from being influenced by oxide; and moreover, through the treatmentmechanism, the metal composite plate production equipment can treat and purify harmful gas released by the metal in the high-temperature and high-pressure environment, and the harmful gas is prevented from corroding parts in the metal composite plate production equipment.
Owner:安徽荣宸汽车零部件制造有限公司

Test paper card for quickly detecting residues of fluoroquinolones and preparation method of test paper card

The invention discloses a test paper card for quickly detecting residues of fluoroquinolones and a preparation method of the test paper card. The key points of the technical scheme of the test paper card are as follows: the test paper card for quickly detecting the residues of the fluoroquinolones comprises a plastic box body and a test strip which is packaged in the plastic box body, wherein the bottom layer of the test strip is used as a supporting layer; a sample pad, a gold-labeled antibody combination pad, a cellulose nitrate film and an absorption pad which are closely connected with one another are adhered on the supporting layer from left to right in sequence; a detection line which is printed by using a norfloxacin and edge albumin conjugate solution and a quality control line which is printed by using a goat anti-rat IgG solution are arranged on the cellulose nitrate film; and colloidal-gold-labeled anti-fluoroquinolone-type specific monoclonal antibodies are poured on the combination pad. The invention also discloses a method for preparing the test paper card for quickly detecting the residues of the fluoroquinolones. The test paper card is easy to operate, fast, accurate, high in sensitivity, low in cost and high in stability, and can be used for performing batch detection.
Owner:HENAN ZHIWEI BIOLOGICAL ENG +1

A kind of leather degreasing agent and its preparation method and application

The invention discloses a leather degreasing agent, a preparation method and application thereof. A degreasing agent comprising one, two, three or more isomeric alcohol block polyethers selected from the following formula (I): RO[CH 2 CH(CH 3 )O] n (CH 2 CH 2 O) m H (I); Wherein, R is selected from the isomeric alkyl group with an average degree of isomerization ≥ 2; n is selected from the number of 1-8; m is selected from the number of 0.5-15. The degreasing agent has good penetration and emulsifying effect, which can effectively reduce the oil content in the leather embryo; the fatliquoring agent is suitable for the processing and degreasing of cowhide, sheepskin, pigskin and other leather products.
Owner:联泓(江苏)新材料研究院有限公司

A kind of rna pulldown method and kit

The invention relates to an RNA pulldown method and a kit. The method includes the following steps of S1, pretreating magnetic beads by means of using BSA and a random oligonucleotide primer to enclose magnetic beads containing streptavidin; S2, preparing probe-magnetic bead complex; S3, extracting and pretreating whole cell proteins by means of adding ribonuclease into whole cell protein extraction samples for incubation, then adding the magnetic beads for incubation, and collecting supernatant; S4, performing pulldown; S5, collecting RNP. The method and the kit have the advantages that the BSA and the random oligonucleotide primer are used for enclosing the magnetic beads in advance, so that nonspecific binding of the magnetic beads with the proteins and RNA is lowered. Through systematic nuclease protection, pollution of nuclease is prevented, stability and reliability in tests are improved, repeatability is good, and test procedures are simplified.
Owner:GUANGZHOU BIOSENSE BIOSCI

A Lectin-Magnetic Carrier Conjugated Complex for the Isolation of Glycosylated Exosomes from Clinical Samples

The present invention relates to a lectin-magnetic carrier coupling complex for separating glycosylated exosomes in clinical samples, the lectin-magnetic carrier coupling complex comprising: a magnetic carrier; Lectin on the outside of the carrier. The lectin-magnetic carrier conjugate complex provided by the present invention can quickly, accurately and automatically separate glycosylated exosomes in clinical samples, with high separation efficiency, and the separated exosomes are complete in shape without rupture or fragmentation, It can be directly used for the detection of glycosylated exosome liquid, or directly for immunological related detection, or directly extract relevant nucleic acids in exosomes for nucleotide sequence detection and analysis.
Owner:北京尧景基因技术有限公司

Polyurethane elastomer material as well as preparation method and application thereof

The invention relates to the field of wear-resistant materials, and particularly discloses a polyurethane elastomer material as well as a preparation method and application thereof. The polyurethane elastomer material is prepared from the following raw materials: polyester polyol and / or polyether polyol, isocyanate, a foaming agent, a chain extender, silicon nitride and modified montmorillonite, the modified montmorillonite is obtained by organically modifying common montmorillonite with acrylamide; the preparation method comprises the following steps: mixing and stirring the polyester polyol and / or polyether polyol and isocyanate to obtain a mixture, mixing and stirring the remaining raw materials to obtain a mixture, adding the mixture into the mixture while stirring to obtain a base material, banburying the base material at 150-200 DEG C for 5-10 minutes to obtain a sizing material, and carrying out compression molding on the sizing material to obtain the polyurethane elastomer material. The polyurethane elastomer material provided by the invention has excellent wear resistance.
Owner:锋特(福建)新材料科技有限公司

A kind of β-diketone organotin PVC heat stabilizer and preparation method thereof

The invention discloses a beta-diketone organotin PVC heat stabilizer and a preparation method thereof, and belongs to the technical field of a PVC heat stabilizer. According to main points of the technical scheme, the beta-diketone organotin PVC heat stabilizer has a structural formula as shown in the specification, wherein R is phenyl or heptadecyl. The invention also discloses a preparation method of the beta-diketone organo tin PVC heat stabilizer. Advantages of an organotin stabilizing agent and a beta-diketone compound auxiliary heat stabilizer are combined, thus achieving the effects of the two substances. Then, the product is more convenient to use. In the preparation method of the beta-diketone organotin PVC heat stabilizer, the use of ultrasonic wave can avoid ammonium chloride which is generated after a reaction and doped in the product from affecting quality of the product; and by the use of nitrogen during the whole reaction, a solvent is not easy to volatilize under the condition of 0 DEG C. through nitrogen for solvent volatilization, crystallization can be promoted, and the influence of oxygen on the combination of a beta-diketone compound and stannous ion can be avoided.
Owner:南通德发生物化工有限公司
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