30results about How to "Has a targeting function" patented technology

A fluorescently-labeled hydrophobic modified oligochitose polymer used for preparing target medicine or fluorescent signal amplifier carrier is prepared through reaction between aqueous solution of oligochitose, fatty acid and cross-linking coupler, and reacting on isothiocyano fluorescent larbel in ice bath, darc and magnetic stirring conditions to obtain said polymer, and ultrasonically dispersing it in water to obtain its colloidal microparticles.

The invention discloses an alimentary resistant starch, the process for preparing the starch, and the use of the starch in preparing oral administration colon targeting medicament control release vectors, wherein the starch preparing process comprises the steps of, (1) mixing the starch with water, (2) heavy pressure modifying the starch or starch milk, (3) reacting in a reactor, (4) subjecting the enzymolysis product yield to centrifugation, scouring, filtering, drying and disintegration.

Hydrophobic nanoparticles have great potential in biological analysis and biomedical application. The invention provides a simple method; a DNA nanostructure is used as a phase transition reagent; the DNA nanostructure is a DNA tetrahedral structure formed by self-assembly of four single-stranded DNAs, tail ends of three single-stranded DNAs of the four single-stranded DNAs are modified by carboxyl, the other one single-stranded DNA contains an aptamer, and the aptamer can be specifically combined with highly expressed nucleolin on a cancer cell membrane surface, so that the targeting ability of the nanoparticles is increased and the cell uptake ability is improved. The DNA tetrahedron as the phase transition reagent is non-toxic and convenient and only requires simple separation, and the high-stability and good-dispersion hydrophilic nanoparticles can be prepared.

The invention relates to a tumor-targeted hollow core-shell structure nano diagnosis-treatment agent and a preparation method thereof. The nano diagnosis-treatment agent comprises an upconversion nanoparticle inner core, an organic-inorganic hybrid silica shell layer and an intermediate cavity structure, can improve the load capacity of a photosensitizer and reduce the photosensitizer aggregation, and can specifically recognize cancer cells by connecting tumor-targeted molecules (such as urokinase ammonia terminal fragments) to the surface; under the irradiation of 980 nm laser, a visible light emission sensitizing photosensitizer of the nano diagnosis-treatment agent generates singlet oxygen, so that the photodynamic therapy of cancer cells can be performed; in addition, the nano diagnosis-treatment agent can be used for the upconversion luminescence and CT dual-mode imaging by utilizing the upconversion luminescence and strong X-ray absorption characteristics.

The invention discloses a contrast medium for fluorescent-magnetic resonance dual-mode targeting biological imaging and a preparation method thereof. The method comprises the following steps that 1, a chelating agent and a compound containing Gd3+ react in water to obtain a Gd3+ chelate solution; 2, citric acid and polyethyleneimine are dissolved into water, and then the Gd3+ chelate solution is added to be mixed; 3, the mixture obtained in the step 2 is dissolved and evaporated to dryness repeatedly, centrifuging is performed on the dissolved and dried products, a supernate is taken for dialysis in water to obtain a dialysis solution, and a dialysis solution is subjected to freeze-drying, and Gd doped carbon dots are obtained. Meanwhile, the invention further discloses a contrast medium for fluorescent-magnetic resonance dual-mode targeting biological imaging. The contrast medium is synthetized in an environment-friendly mode, is low in gadolinium content, promotes surface functionalization, and has the quite high longitudinal relaxation rate and the fluorescence quantum yield, and the good biological compatibility and blood compatibility.

The invention discloses a preparation method and application of an RGD@BBN double-targeted MR (magnetic resonance) / optical dual-mode molecular probe, wherein the RGD@BBN double-targeted MR / optical dual-mode molecular probe is prepared by the following steps: coating water-soluble superparamagnetic iron oxide ( SPIO) particles in hydrophilic core of lipidosome and coating oil-soluble QDs particles in hydrophobic lipid bilayer of the lipidosome by taking lipidosome as a carrier, and linking a double-targeted RGD@BBN ligand by virtue of a post-interpolation method. The RGD@BBN contained in the dual-mode molecular probe disclosed by the invention is interpolated and immobilized on the surface of the lipid bilayer by virtue of self-assembly, and the preparation method is mild in condition and is efficient and practical. According to the invention, a relatively independent space is coated by two different image contrast agents without changing the properties of the image contrast agents; and the prepared double-target dual-mode molecular probe, which simultaneously has RGD and BBN targeting functions, has targeting functions on RGD or (and) BBN receptor-positive tumors.

The invention discloses a protein nanoparticle containing a bioactivity oligopeptide-ferritin heavy chain subunit and a preparation method of the protein nanoparticle. The preparation method comprises the following steps: amplifying a fragment of a fusion gene of the bioactivity oligopeptide-ferritin heavy chain subunit by adopting an overlapping PCR (polymerase chain reaction) technology, inserting the amplified fragments into a pET28a(+) carrier, and transforming the carrier into a DH5 alpha competent cell to obtain expression engineering bacteria and an inclusion body; uniformly mixing the diluted bioactivity oligopeptide-ferritin heavy chain subunit inclusion body and an EGF (epidermal growth factor)-ferritin heavy chain subunit inclusion body according to a molar ratio of 6:4, and performing dialysis and renaturation on the uniformly mixed solution in a dialysis bag to obtain the multi-functionalized protein nanoparticle containing the bioactivity oligopeptide-ferritin heavy chain subunit. The obtained protein nanoparticle has a targeting function and also has spacing and stabilizing effects on modification of bioactivity oligopeptides; the interaction among oligopeptides is avoided, so that the functionalization of the protein nanoparticle is made possible.

The invention discloses a multifunctional probe and a preparation method and application thereof. The nano diagnosis and treatment probe with the two functions of magnetic resonance imaging and thermal therapy is formed in the mode that nano gold bars are formed by adding surfactant, chloroauric acid, a reduction agent and silver nitrate, target macromolecules are inoculated, and gadolinium ions are adsorbed. The preparation method is simple, the probe is good in dispersity in an aqueous solution, good in stability and capable of meeting requirements of clinical application and combining radiography and thermal therapy, and the dual functions of lymphoma diagnosis and treatment are achieved.

The invention discloses an anti-microbial lipopenic compound with a target function and a preparation method thereof. The anti-microbial lipopenic compound is prepared from lipopeptid and neoglycolipid which are self assembled in solution, wherein the lipopeptid and the neoglycolipid are similar in structure, the lipopeptid is prepared from bioactive peptide and a hydrophobic chain section, and the neoglycolipid is prepared from oligosaccharide and a similar hydrophobic chain section. According to the anti-microbial lipopenic compound with the target function, a glycosyl unit in the neoglycolipid is utilized as a chemotactic agent to target bacterial membranes, and the anti-microbial lipopeptid plays an anti-microbial role; thus, the anti-microbial lipopenic compound has the characteristics of being high in anti-microbial activity, long in action time, low in dosage and not prone to generating drug resistance.

The invention discloses a preparation method of a hypocrellin-transferrin targeted drug delivery system and an application of the hypocrellin-transferrin targeted drug delivery system in photodynamic therapy. The method comprises the following steps: with holo-transferrin as a carrier, inactivating the holo-transferrin by urea and beta-mercaptoethanol, and making a three-dimensional structure loose to expose Fe-ion coordination sites; adding excessive hypocrellin, coordinating with Fe coordination sites, eliminating uncombined HA and urea through dialysis, and restoring the three-dimensional structure of holo-transferrin with beta-mercaptoethanol, thereby obtaining the hypocrellin-transferrin targeted drug delivery system. The method is simple in preparation and good in application prospect; the hypocrellin-transferrin targeted drug delivery system can be prepared into freeze-dried powder for storage, and the freeze-dried powder is prepared into a preparation suitable for intravenous injection after being dissolved. Due to the tumor targeting action of holo-transferrin in the system, the photosensitive anti-tumor activity of hypocrellin can be significantly strengthened by the targeted drug delivery system.

The invention discloses polyamine phthalocyanine and a derivative thereof. The structural general formula of the derivative of polyamine phthalocyanine is shown in descriptions. The derivative of polyamine phthalocyanine, disclosed by the invention, has relatively good solubility and photosensitive activity, has a certain targeting function and is suitable for serving as a photosensitizer for PDT (Photodynamic Therapy). The invention further relates to preparation methods and application of polyamine phthalocyanine and the derivative thereof.

The invention provides a chromene pyridine derivative fluorescent probe as well as a preparation method and application thereof. The structural formula of the fluorescent probe is shown in the specification. The specific preparation method comprises the following steps: dissolving 3H-benzo[f]chromene-2-formaldehyde and 4-methyl-1-(2-morpholine-4-ethyl)-pyridine bromide in ethanol, dropwise addingpiperidine as a catalyst, carrying out reflux stirring at 80 DEG C for 4-5 hours, cooling, standing to room temperature, carrying out reduced pressure suction filtration, and cleaning the obtained solid with ethanol to obtain the chromene pyridine derivative fluorescent probe. The chromene pyridine derivative fluorescent probe can selectively react with hypochlorite under pure water phase physiological conditions, the solution is yellow and fades, meanwhile, red fluorescence is remarkably weakened, and the chromene pyridine derivative fluorescent probe is particularly applied to convenient detection of hypochlorite in cytolysosome as a fluorescent probe. The probe does not need any organic solvent for hydrotropy in a working environment, is very beneficial to being applied to a biologicalsystem, and has wide potential application value.

The invention discloses a multifunctional underwear fabric containing active polypeptide, underwear prepared from the multifunctional underwear fabric and a preparation method of the multifunctional underwear fabric. The method comprises the steps: 1, pretreating fabric with the surface containing hydroxyl and / or amino groups; 2, activating reaction groups on the fabric by adopting a chemical reagent; 3, detecting the density of the activated groups on the fabric; 4, reacting the activated fabric with active polypeptide; 5, cleaning the fabric; 6, naturally air-drying or drying the fabric at low temperature; 7, cutting the fabric according to a underwear sample plate; 8, conveying the fabric to a garment production line for sewing; and 9, carrying out high-temperature ironing on sewn underwear, packaging and warehousing. The invention further provides the multifunctional underwear fabric containing the active polypeptide and underwear prepared through the method. According to the invention, the active polypeptide is utilized to modify the existing fabric, so that the underwear produced by utilizing the fabric has the functions of protecting skin, beautifying skin, repairing skin surface inflammation and the like.

The invention discloses an alimentary resistant starch, the process for preparing the starch, and the use of the starch in preparing oral administration colon targeting medicament control release vectors, wherein the starch preparing process comprises the steps of, (1) mixing the starch with water, (2) heavy pressure modifying the starch or starch milk, (3) reacting in a reactor, (4) subjecting the enzymolysis product yield to centrifugation, scouring, filtering, drying and disintegration.

The invention discloses the preparation and application of a RGD@BBN dual-targeting MR / optical dual-mode molecular probe. In the present invention, liposomes are used as carriers, water-soluble SPIO particles are wrapped in liposome hydrophilic core, oil-soluble QDs particles are wrapped in liposome hydrophobic phospholipid bilayer, and double-targeted RGD@ BBN ligand, preparation of RGD@BBN dual-targeting MR / optical dual-modal molecular probe. The RGD@BBN contained in the bimodal molecular probe of the present invention is inserted and fixed on the surface of the phospholipid bilayer by a self-assembly method, the preparation conditions are mild, and it is efficient and practical. The present invention wraps two different image contrast agents in a relatively independent space without changing their properties, and the prepared dual-target dual-mode molecular probe has both RGD and BBN targeting functions, and is effective for RGD or ( and) BBN receptor-positive tumors all have targeting function.

The invention discloses polyamine phthalocyanine and a derivative thereof. The structural general formula of the derivative of polyamine phthalocyanine is shown in descriptions. The derivative of polyamine phthalocyanine, disclosed by the invention, has relatively good solubility and photosensitive activity, has a certain targeting function and is suitable for serving as a photosensitizer for PDT (Photodynamic Therapy). The invention further relates to preparation methods and application of polyamine phthalocyanine and the derivative thereof.

The present invention provides a targeting carrier for treating pancreatic cancer, which includes: CD44v6 single-chain antibody for targeted recognition of pancreatic cancer cells, and nanocarrier IONP-PEI; wherein CD44v6 single-chain antibody and nanocarrier IONP-PEI PEI-coupled. In another aspect, the present invention provides a nanoparticle for treating pancreatic cancer, which includes the aforementioned targeting carrier, silencing RNA, and gemcitabine chemotherapeutic drug, wherein the silencing RNA and gemcitabine are complexed on the aforementioned targeting carrier. The invention constructs a non-viral gene carrier with high efficiency, safety and targeting function. Coupling silencing RNA and gemcitabine through nanocarriers and anti-CD44v6 single-chain antibody, combining gene therapy, chemotherapy and biological targeted therapy, provides new ideas and scientific basis for the development of new anti-pancreatic cancer drugs.

The invention discloses a multifunctional probe and a preparation method and application thereof. The nano diagnosis and treatment probe with the two functions of magnetic resonance imaging and thermal therapy is formed in the mode that nano gold bars are formed by adding surfactant, chloroauric acid, a reduction agent and silver nitrate, target macromolecules are inoculated, and gadolinium ions are adsorbed. The preparation method is simple, the probe is good in dispersity in an aqueous solution, good in stability and capable of meeting requirements of clinical application and combining radiography and thermal therapy, and the dual functions of lymphoma diagnosis and treatment are achieved.

The invention belongs to the technical field of biomedical materials, and discloses a UCST type polymer with a bacterium targeting function as well as a preparation method and application thereof. The method comprises the following steps: by taking an organic solvent as a reaction medium, carrying out polymerization reaction on acrylamide, acrylonitrile and a modified glucose monomer under the action of a chain transfer agent and an initiator, and removing acetyl to obtain the UCST polymer with the bacterial targeting function. The structure of the polymer is as shown in formula I. The polymer has the highest critical solution temperature and good biocompatibility. The polymer promotes the formation of bacterial aggregates and realizes a bacterial targeting function. The polymers of the present invention are useful as antibacterial carriers. The polymer has good antibacterial drug (photo-thermal reagent) coating capacity, and after the polymer is loaded with the photo-thermal reagent, the in-situ sterilization effect of the photo-thermal reagent is greatly enhanced through the targeting function of the polymer on bacteria under infrared light irradiation.

The invention provides a preparation method of a magnetic-fluorescent functional nano bio-compound with mycotoxin targeting effect. The preparation method includes following steps: preparing a water-soluble CdTe quantum dot (CdTe QDs for short) water solution; preparing Fe3O4 magnetic nanosphere dispersion liquid; preparing SiO2-coated Fe3O4 (Fe3O4@SiO2 for short) magnetic nanosphere dispersion liquid; preparing CdTe QDs-coated Fe3O4@SiO2 difunctional nanosphere (Fe3O4@SiO2@CdTe QDs for short) dispersion liquid; preparing Fe3O4@SiO2@CdTe QDs-marked nano bio-compound dispersion liquid with a mycotoxin targeting function. SiO2 shell layer coating is performed on the surfaces of Fe3O4 magnetic nanospheres to prepare Fe3O4@SiO2 magnetic composite spheres, so that stability, chemical modification performance and biocompatibility of a magnetic control carrier are improved effectively.

The invention relates to the technical field of high polymer materials, in particular to a preparation method and application of a fluorogold nanocluster coupled Napsin A compound. Comprising the following steps: adding 1-ethyl-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide into a flask of N, N-dimethylformamide, stirring for several minutes, adding 3-mercaptopropionic acid, continuously stirring, and dropwise adding an amino-containing high-molecular long-chain polymer solution under the condition of violent stirring; according to the preparation method and the application of the fluorescent gold nano-cluster coupled Napsin A compound, bright red fluorescent gold nano-clusters are prepared, then 1-ethyl-(3-dimethylaminopropyl) carbodiimide / N-hydroxysuccinimide is utilized for reaction to be coupled with a Napsin A antibody, the compound with a targeting function is obtained, and early diagnosis and intraoperative positioning of lung cancer are carried out.

The invention discloses a preparation method for giant magnetic-responsiveness medicine-carrying vesicles with a targeting function, and relates to a preparation method for medicine-carrying vesicles. The preparation method disclosed by the invention aims at solving the defects of high toxic and side effects, poor targeting effect on tumour tissues and poor selectivity on cell killing in the existing medicines for treating tumour diseases. The preparation method comprises the following steps: 1, preparing a phospholipid solution; 2, preparing ITO conductive glass with a surface covered with a phospholipid layer; 3, preparing a mixed solution of medicines and nano-particles; 4, assembling an airtight preparation device to react, so as to obtain the giant magnetic-responsiveness medicine-carrying vesicles with the targeting function. The giant magnetic-responsiveness medicine-carrying vesicles with the targeting function prepared by the preparation method disclosed by the invention have sizes up to 1-100mu m, high medicine-carrying amount, and the characteristics of being easy for carrier separation and purification, good in bio-security, high in release efficiency and controllable in release. The invention discloses a preparation method capable of obtaining giant magnetic-responsiveness medicine-carrying vesicles with a targeting function.

The invention discloses a preparation method and an application method of double biological optical window (650-1000nm) targeted nanometer biological probe. The preparation method comprises the following steps of performing organic metal catalyzed Heck reaction with a method of adopting bicomponent alternating copolymerization on a divinyl fluorene derivative and aromatic heterocyclic groups withnarrow band gaps to obtain a neutral polymer, and then performing quaternization to obtain a cationic water-soluble conjugated polymer with a main chain being of a D-pi-A structure and with two-photonabsorption and near infrared-emitting properties simultaneously, wherein an excitation wavelength and a emission wavelength of the cationic water-soluble conjugated polymer are in biological opticalwindows (i.e., double biological optical windows); and performing a nanoprecipitation method on the cationic water-soluble conjugated polymer and tumor-targeted biomolecules with opposite charges to prepare the targeted nanometer biological probe. By adopting the preparation method and the application method, double biological optical window targeted two-photon fluorocyte imaging can be realized.

The invention discloses a preparation method and an application method of double biological optical window (650-1000nm) targeted nanometer biological probe. The preparation method comprises the following steps of performing organic metal catalyzed Heck reaction with a method of adopting bicomponent alternating copolymerization on a divinyl fluorene derivative and aromatic heterocyclic groups withnarrow band gaps to obtain a neutral polymer, and then performing quaternization to obtain a cationic water-soluble conjugated polymer with a main chain being of a D-pi-A structure and with two-photonabsorption and near infrared-emitting properties simultaneously, wherein an excitation wavelength and a emission wavelength of the cationic water-soluble conjugated polymer are in biological opticalwindows (i.e., double biological optical windows); and performing a nanoprecipitation method on the cationic water-soluble conjugated polymer and tumor-targeted biomolecules with opposite charges to prepare the targeted nanometer biological probe. By adopting the preparation method and the application method, double biological optical window targeted two-photon fluorocyte imaging can be realized.

The invention provides curcumin tannin nanoparticles, a preparation method and application thereof, and belongs to the field of biomedical materials. The nano particle is prepared from raw materials in the following weight ratio: 80-100 parts of human serum albumin, 10-50 parts of genipin, 1-20 parts of medicine and 10-50 parts of tannic acid. The drug is preferably curcumin. The tannic acid drug-loaded nanoparticle raw material of the present invention is derived from food-grade materials, and its toxicity is much lower than other synthetic chemical nanomaterials; the nanoparticle has good stability, high encapsulation efficiency and drug loading rate; more importantly, the present invention The invented nanoparticle has a targeting function to the diseased colon, and the nanoparticle can be protected from the destructive influence of the gastric environment, making its effect better than other previously reported nano-drug delivery systems. Therefore, the present invention provides a more promising oral drug delivery system for the treatment of colonic sites, especially ulcerative colitis.