Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fluorescence labeling hydrophobic modified chitin polymer, its preparation method and application

A hydrophobic modification and fluorescent labeling technology, used in pharmaceutical formulations, medical preparations with inactive ingredients, etc., can solve the problems of high molecular weight and high viscosity, and achieve the effect of simple method, mild reaction conditions, and guaranteed biocompatibility.

Inactive Publication Date: 2007-05-30
ZHEJIANG UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commercially available chitosan has the disadvantages of large molecular weight (hundreds of thousands), high viscosity, and inability to dissolve under physiological pH (7.4) conditions.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescence labeling hydrophobic modified chitin polymer, its preparation method and application
  • Fluorescence labeling hydrophobic modified chitin polymer, its preparation method and application
  • Fluorescence labeling hydrophobic modified chitin polymer, its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0027] 1. Preparation of chitosan oligosaccharide

[0028] Add 6g of chitosan (average molecular weight 450kDa) to 200mL of 1.25% (v / v) hydrochloric acid aqueous solution, stir and dissolve at 55-60°C, adjust the pH to 5.0 with dilute ammonia water or dilute hydrochloric acid, press cellulase and shell The ratio of polysaccharides is 0.5:100 (w / w) to add cellulase, and after controlling the reaction time for 8 hours, the reaction product is 4000r×min -1 After centrifugation for 10 minutes, the supernatant was pretreated with a 0.45 μm microporous membrane, graded by ultrafiltration with ultrafiltration membranes of different molecular weights, and the ultrafiltrate was freeze-dried to obtain chitosan oligosaccharides with a certain molecular weight. And the average molecular weight was determined to be 50.6 kDa by gel permeation chromatography.

[0029] 2. Preparation of fluorescently labeled hydrophobic modified chitosan oligosaccharides

[0030] Get above-mentioned chitosa...

example 2

[0037] 1. Preparation of chitosan oligosaccharide

[0038] Add 6g of chitosan (average molecular weight 450kDa) to 200mL of 1.25% (v / v) hydrochloric acid aqueous solution, stir and dissolve at 55-60°C, adjust the pH to 5.0 with dilute ammonia water or dilute hydrochloric acid, press cellulase and shell Add cellulase at a glycan ratio of 0.5:100 (w / w), and control the reaction time for 24 hours. -1 After centrifugation for 10 minutes, the supernatant was pretreated with a 0.45 μm microporous membrane, graded by ultrafiltration with ultrafiltration membranes of different molecular weights, and the ultrafiltrate was freeze-dried to obtain chitosan oligosaccharides with a certain molecular weight. And the average molecular weight was determined to be 10.1 kDa by gel permeation chromatography.

[0039] 2. Preparation of fluorescently labeled hydrophobic modified chitosan oligosaccharides

[0040] Take 0.5g chitosan (average molecular weight 10.1kDa), accurately weighed. After ad...

example 3

[0046] 1. Preparation of chitosan oligosaccharide

[0047] Add 6g of chitosan (average molecular weight 450kDa) to 200mL of 1.25% (v / v) hydrochloric acid aqueous solution, stir and dissolve at 55-60°C, adjust the pH to 5.0 with dilute ammonia water or dilute hydrochloric acid, press cellulase and shell Add cellulase at a glycan ratio of 0.5:100 (w / w), and control the reaction time for 20 hours. -1 After centrifugation for 10 minutes, the supernatant was pretreated with a 0.45 μm microporous membrane, graded by ultrafiltration with ultrafiltration membranes of different molecular weights, and the ultrafiltrate was freeze-dried to obtain chitosan oligosaccharides with different molecular weights. And the average molecular weight was determined to be 18.8 kDa by gel permeation chromatography.

[0048] 2. Preparation of fluorescently labeled hydrophobic modified chitosan oligosaccharides

[0049] Get 0.5g chitosan oligosaccharide (average molecular weight 18.8kDa), accurately we...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
electric potential / voltageaaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

A fluorescently-labeled hydrophobic modified oligochitose polymer used for preparing target medicine or fluorescent signal amplifier carrier is prepared through reaction between aqueous solution of oligochitose, fatty acid and cross-linking coupler, and reacting on isothiocyano fluorescent larbel in ice bath, darc and magnetic stirring conditions to obtain said polymer, and ultrasonically dispersing it in water to obtain its colloidal microparticles.

Description

technical field [0001] The invention belongs to a preparation method of a compound, and relates to a preparation method of a fluorescently labeled hydrophobically modified chitosan oligosaccharide polymer and its application in the field of life sciences as a carrier of nanometer micelles. Background technique [0002] In recent years, microparticle carriers have received more and more attention and applications in the field of drug targeting. For example, liposomes, nanoparticles, microspheres and polymer micelles have been studied more. These particulate carriers are considered to be an excellent targeted drug delivery system due to their small particle size, long-term circulation in the body, good biocompatibility, and biomembrane penetration. In particular, it has unique advantages in macromolecular drugs and gene delivery, and is a hot spot in the current research field. [0003] Drug-targeted therapeutics divides drug-targeted therapy into three stages of development...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08A61K47/36
Inventor 胡富强叶轶青袁虹
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com