The invention relates to the technical field of reuse of waste fungus chaff after edible fungi cultivation and particularly relates to flammulina velutipes fungus chaff feed and a production method and an application thereof. The flammulina velutipes fungus chaff feed comprises a mixture a and a mixture b, wherein the mass ratio of the mixture a to the mixture b is (1000:1)-(1000:10); the mixture a consists of the following raw materials: flammulina velutipes fungus chaff powder, sweet potato dreg powder, rice bran, wheat flour, soya bean meal, fish meal or meat and bone meal and table salt; and the mixture b consists of the following raw materials: cellulase, hemicellulase, aspergillus niger, ligninase, cutinase, feruloyl esterase and brown sugar. The production method of the flammulina velutipes fungus chaff feed is simple. The flammulina velutipes fungus chaff feed is used for culturing barley pests, the time required by culturing the barley pests is short, the weights of the barley pests are heavy, and a plurality of eggs can be laid by the barley pests. The cultured barley pests are added into the conventional feed and are used for feeding beasts and birds, crabs, fishes, turtles, eels, finless eels or batrachia, the growth of a fed animal is obviously accelerated, the increase production is remarkable, and the effect is good.