The invention discloses a cell culture medium. The cell culture medium is prepared from components as follows: 80v / v%-90v / v% of a DMEM / F12 culture medium, 25-500 mg / ml of a sericin solution, 2.0-5 mg / ml of a chitosan quaternary ammonium salt solution, 35-70 mg / ml of human serum albumin, 20-80 mg / ml of phytohemagglutinin, 20-100 mu g / ml of vitamin C, 20-80 mu g / ml of vitamin E, 0.5-10 ng / ml of an epidermal growth factor, 5-20 ng / ml of an insulin-like growth factor and 1-5 mmol / ml of L-glutamine. Cell growth can be promoted by use of sericin, and the chitosan quaternary ammonium salt solution isused for the cell culture medium, can improve the cell proliferation capability and tumor killing rate and can improve the cell growth capability when matched with sericin for use. Kidney bean lectinhas the functions of agglutinating blood cells, lymphocyte, spermatid and the like, and in in-vitro cell culture, phytolectin can proliferate lymphocyte; in the whole experiment, the phytolectin canimprove the immune capability of a body; under the synergistic action of sericin, the chitosan quaternary ammonium salt solution and lectin, the proliferation rate, the survival rate and the tumor killing rate of T lymphocyte are remarkably increased.