Hyriopsis cumingii enzymolysis polypeptide, as well as preparation and application thereof
A technology of spina mussels and sail mussels enzyme, which is applied in the application of enhancing immunity function. It takes a long time to achieve the effect of convenient industrial production, stable process and easy preservation
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Embodiment 1
[0024] Preparation method of mussel enzymatic polypeptide (MPP)
[0025] After picking the pearls, the fresh clam meat is taken out, mashed and beaten into a slurry, mixed with water at 1:5-6 (mass ratio), boiled for 15-20min, filtered while hot, and the supernatant is removed ( Most of them are mussel meat polysaccharides). After the precipitation is dried and defatted, mussel protein powder is obtained. Add appropriate amount of water to fully soak for about 12 hours, superfinely pulverize and homogenize, and adjust the pH to 8.0-9.0 (the ratio of powder to water is controlled at 1:6 -8), add alkaline protease (Alcalase) with the enzyme amount of 0.3%-0.6% (calculated by protein content), react at 40-60℃ for 4-6 hours, inactivate the enzyme at 85-90℃ for 15 minutes, centrifuge, After being concentrated and dried at night, the Hyriopsis cumingii enzymatically hydrolyzed polypeptide (MPP) is obtained.
[0026] Hyriopsis enzymatically hydrolyzed polypeptide (MPP), its total protein...
Embodiment 2
[0032] Example 1 Determination of the in vitro immunological activity of the enzymatically hydrolyzed polypeptide of Hyriopsis cumingii (hereinafter referred to as polypeptide)
[0033] 1. Principles of in vitro immunization experiments
[0034] Concanavalin A (ConA) is a mitogen, which can stimulate new DNA synthesis and cell differentiation in T lymphocytes to cause cell proliferation. The experiment uses the thiazole salt colorimetric method (MTT method) to detect the transformation function of lymphocytes. MTT colorimetry is a method to detect cell survival and growth. Succinate dehydrogenase in living cells can reduce exogenous MTT to blue-purple crystals. When the mitochondrial membrane is damaged, the amount of MTT reduced is significantly reduced, and dead cells There is no such function. Dimethyl sulfoxide (DMSO) can dissolve purple crystals in cells, and measure its light absorption value at a certain wavelength with a microplate reader, which can directly reflect the me...
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