The invention discloses an improved
model organism zebra fish
osteoporosis model application method and application of the improved
model organism zebra fish
osteoporosis model application method in screening the
traditional Chinese medicine anti-
osteoporosis active component by uniting with the modern
chromatography technology. According to the improved
model organism zebra fish osteoporosis
model application method, the features of high-efficiency separation and analysis of traditional Chinese components of the modern
chromatography technology and the united technology of the modern
chromatography technology are combined, a target component or component groups are captured or knocked out or authenticated directionally. The component or the component groups which are captured or knocked out are screened in an anti-osteoporosis
active mode with the zebra fish osteoporosis model. The traditional Chinese components are separated and analyzed according to the optimized chromatography and
mass spectrum conditions. The traditional Chinese components are analyzed according to features of
ultraviolet ray absorption, molecular weight and the like. The target component or the component groups are flexibly determined as required, the target component or the component groups of different preserving time periods are collected and thus, the captured or knocked-out
traditional Chinese medicine component or the component groups are obtained. After an
organic solvent is removed, a proper
solvent is used for carrying out
dissolution. The zebra fish osteoporosis model which carries out inducing with glucocorticoids rapidly evaluates anti-osteoporosis activity of the traditional Chinese component or the component groups. The modern chromatography and the united technology of the modern chromatography overcome the defects that a traditional chemical component is low in separation speed and a large number of trace components are hard to obtain. The zebra fish osteoporosis model breaks through the
bottleneck that the trace components are hard to undergo anti-osteoporosis activity high-efficiency evaluation. The modern chromatography and the united technology are united in an organic mode so as to break through the restraint of an evaluation model and the number of chemical compounds. The anti-osteoporosis pharmacodynamic material basis is provided for screening the
traditional Chinese medicine efficiently.