Novel method for screening traditional Chinese medicine anti-osteoporosis active component with zebra fish osteoporosis model

Abstract

The invention discloses an improved model organism zebra fish osteoporosis model application method and application of the improved model organism zebra fish osteoporosis model application method in screening the traditional Chinese medicine anti-osteoporosis active component by uniting with the modern chromatography technology. According to the improved model organism zebra fish osteoporosis model application method, the features of high-efficiency separation and analysis of traditional Chinese components of the modern chromatography technology and the united technology of the modern chromatography technology are combined, a target component or component groups are captured or knocked out or authenticated directionally. The component or the component groups which are captured or knocked out are screened in an anti-osteoporosis active mode with the zebra fish osteoporosis model. The traditional Chinese components are separated and analyzed according to the optimized chromatography and mass spectrum conditions. The traditional Chinese components are analyzed according to features of ultraviolet ray absorption, molecular weight and the like. The target component or the component groups are flexibly determined as required, the target component or the component groups of different preserving time periods are collected and thus, the captured or knocked-out traditional Chinese medicine component or the component groups are obtained. After an organic solvent is removed, a proper solvent is used for carrying out dissolution. The zebra fish osteoporosis model which carries out inducing with glucocorticoids rapidly evaluates anti-osteoporosis activity of the traditional Chinese component or the component groups. The modern chromatography and the united technology of the modern chromatography overcome the defects that a traditional chemical component is low in separation speed and a large number of trace components are hard to obtain. The zebra fish osteoporosis model breaks through the bottleneck that the trace components are hard to undergo anti-osteoporosis activity high-efficiency evaluation. The modern chromatography and the united technology are united in an organic mode so as to break through the restraint of an evaluation model and the number of chemical compounds. The anti-osteoporosis pharmacodynamic material basis is provided for screening the traditional Chinese medicine efficiently.

Description

technical field [0001] The invention relates to a new application method for establishing an osteoporosis model with the model organism zebrafish, especially the application of its combined chromatographic technology in the high-efficiency screening of anti-osteoporosis active ingredients of traditional Chinese medicines, in particular to the use of the zebrafish osteoporosis model combined with Modern chromatography such as high performance liquid chromatography or its combined techniques such as mass spectrometry can effectively screen the active ingredients of traditional Chinese medicine for anti-osteoporosis. Background technique [0002] The composition of traditional Chinese medicine is complex, with the characteristics of "multiple components, multiple targets, and overall effect". What method and technology to use to quickly and efficiently clarify the material basis of traditional Chinese medicine is a hot and difficult issue that scholars at home and abroad are co...

AI Technical Summary

Problems solved by technology

However, the anti-osteoporosis activity screening of traditional Chinese medicine ingredients, especially micro-components, is still difficult to carry out simply and efficiently. There are two bottlenecks: one is the evaluation model problem: the existing mammalian osteoporosis model takes a long time and requires a large amount of samples , labor-intensive, low efficiency, not suitable for high-efficiency activity screening; the existing osteoblast, osteoclast and tumor cell replacement models have a single action link, which is difficult to reflect the overall view of traditional Chinese medicine; in addition, the cost is high, the experimental conditions are relatively harsh, and primary cells The difficulty of cultivation increases, and it is difficult for ordinary laboratories to carry out; the second is the problem of the number of compounds: due to the complexity of traditional Chinese medicine components, it is difficult to separate enough compounds to meet the requirements of in vivo activity evaluation, and the enrichment of microcomponents and in vivo metabolic components , more difficult to separate

Combining the zebrafish osteoporosis model with modern chromatography can break through the constraints of the evaluation model and the number of compounds in the anti-osteoporosis screening process of traditional Chinese medicine. This aspect of research is still blank in China

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