Method for evaluating quality of Herba Cirsii Setosi through quantitative analysis of multicomponents by single marker

A small thistle and quality technology, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of many varieties of standard products, complex content determination methods, and high detection economic costs, achieve high detection sensitivity, and reduce detection costs and detection time. , The effect of simplifying the quantitative detection method

Inactive Publication Date: 2019-07-09
XIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for evaluating the quality of thistle with one test and multiple evaluation methods, which solves the problems of many standard products in the determination of conten...

Method used

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  • Method for evaluating quality of Herba Cirsii Setosi through quantitative analysis of multicomponents by single marker
  • Method for evaluating quality of Herba Cirsii Setosi through quantitative analysis of multicomponents by single marker
  • Method for evaluating quality of Herba Cirsii Setosi through quantitative analysis of multicomponents by single marker

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Experimental program
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Effect test

Embodiment 1

[0050] The multi-component detection method of thistle in the present invention adopts the method of one test and multiple evaluations, and uses rutin as an internal reference to measure the contents of succulentin, luteolin and apigenin in thistle, specifically comprising the following steps:

[0051] Step 1, preparation of reference substance standard solution

[0052] Step 1.1. Accurately weigh the rutin standard, mongoside standard, luteolin standard, and apigenin standard, respectively, and place them in 10mL measuring bottles, add methanol to dissolve, and set the volume to the mark to obtain a concentration of 0.50mg The rutin standard solution in / mL, the mongoside standard solution with a concentration of 0.05mg / mL, the luteolin standard solution with a concentration of 0.51mg / mL and the apigenin standard solution with a concentration of 0.41mg / mL;

[0053] Step 1.2. Take 1 mL each of the above-mentioned rutin, mongoside, luteolin and apigenin standard solution respec...

Embodiment 2

[0091] The multi-component detection method of thistle in the present invention adopts the method of one test and multiple evaluations, and uses rutin as an internal reference to measure the contents of succulentin, luteolin and apigenin in thistle, specifically comprising the following steps:

[0092] Step 1, preparation of reference substance standard solution

[0093] Step 1.1. Accurately weigh the rutin standard, mongoside standard, luteolin standard, and apigenin standard, respectively, and place them in 10mL measuring bottles, add methanol to dissolve, and set the volume to the mark to obtain a concentration of 0.50mg The rutin standard solution in / mL, the mongoside standard solution with a concentration of 0.05mg / mL, the luteolin standard solution with a concentration of 0.51mg / mL and the apigenin standard solution with a concentration of 0.41mg / mL;

[0094] Step 1.2. Take 1 mL each of the above-mentioned rutin, mongoside, luteolin and apigenin standard solution respec...

Embodiment 3

[0119] The multi-component detection method of thistle in the present invention adopts the method of one test and multiple evaluations, and uses rutin as an internal reference to measure the contents of succulentin, luteolin and apigenin in thistle, specifically comprising the following steps:

[0120] Step 1, preparation of reference substance standard solution

[0121] Step 1.1, accurately weigh the standard products of rutin, mongoside, luteolin and apigenin, place them in 10mL measuring bottles respectively, add methanol to dissolve, set the volume to the mark, and obtain rutin with a concentration of 0.50mg / mL Standard solution, a concentration of 0.05mg / mL of the standard solution of mongoside, a concentration of luteolin standard solution of 0.51mg / mL and a concentration of 0.41mg / mL of apigenin standard solution;

[0122] Step 1.2. Take 1 mL each of the above-mentioned rutin, mongoside, luteolin and apigenin standard solution respectively, put them in a 10 mL volumetri...

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Abstract

The invention provides a method for evaluating quality of Herba Cirsii Setosi through quantitative analysis of multicomponents by a single marker. According to the method for evaluating the quality ofthe Herba Cirsii Setosi through the quantitative analysis of multicomponents by the single marker, firstly, a HPLC content determination method for four main medicinal components in the Herba CirsiiSetosi is established; rutin is used as an internal reference substance, relative correction factors of another three active components are calculated, and the system suitability and the method reproducibility of the relative correction factors are investigated; chromatographic peak positioning is carried out according to relative retention time, the relative correction factors are combined for calculating the content of all to-be-detected components, through the mutual verification of the quantitative analysis of multicomponents by the single marker and an external standard method, it is proved that no significant difference exists in determination results; the problems that reference substances are high in costs and are not easy to obtain are solved, the content of index components is calculated through the relative correction factors and the chromatographic peak positioning, and the simultaneous determination of multiple medicinal substances in the Herba Cirsii Setosi is realized; and the costs can be saved, the operation can be simplified, the efficiency is improved, the detection sensitivity is high, the stability is good, the determination results are exact and reliable, andthe method has great significance for ensuring the quality control and the clinical effects of the Herba Cirsii Setosi.

Description

technical field [0001] The invention belongs to the technical field of drug quality control, and relates to a method for evaluating the quality of thistle with one test and multiple evaluations. Background technique [0002] Small thistle Herba Cirsii Setosi, commonly known as thorn horn bud, thorn horn, is the dry aboveground part of the plant Herba Cirsii Setosi in the family Asteraceae, and is a commonly used traditional Chinese medicine. Thistle is sweet in taste, bitter in taste, cool in nature, returns to the heart and Liver Meridian, and has the effects of cooling blood to stop bleeding, dispelling blood stasis and reducing swelling. It can be used for the treatment of epistaxis, hematemesis, hematuria, hematochezia, metrostaxis, traumatic bleeding, carbuncle and sore. The crude extract of thistle has obvious antibacterial and anti-inflammatory activities, and has inhibitory effects on pneumococcus, hemolytic streptococcus, diphtheria, typhoid, Pseudomonas aeruginosa...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张寒郑梦迪张彦刘春叶刘洁翟西峰
Owner XIAN MEDICAL UNIV
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