Method for evaluating quality of Ampelopsis japonic(Thunb.)Makino through quantitative analysis of multicomponents by single marker

A technology of whiteness and quality, which is applied in the field of drug quality control, can solve the problems of complex content determination methods, high economic cost of detection, and many varieties of standard products, and achieve the effects of easy promotion, simplified quantitative detection methods, and reduced detection time

Inactive Publication Date: 2019-07-09
XIAN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide a kind of method of evaluating the quality of scallops by one test and multiple evaluations, which solves the problems in the prior art that there are many kinds of standard products in the content determination of scorpion, the method of content determination is complicated, and the problem of high detection economic cost is solved. Simplifies the quantitative detection method of basilisk and saves the detection economic cost of the quality control of basilisk

Method used

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  • Method for evaluating quality of Ampelopsis japonic(Thunb.)Makino through quantitative analysis of multicomponents by single marker
  • Method for evaluating quality of Ampelopsis japonic(Thunb.)Makino through quantitative analysis of multicomponents by single marker
  • Method for evaluating quality of Ampelopsis japonic(Thunb.)Makino through quantitative analysis of multicomponents by single marker

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Experimental program
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Embodiment 1

[0056] The method of evaluating the quality of Baishui with one test and multiple evaluations adopts the method of one test and multiple evaluations, and uses resveratrol as an internal reference to establish relative correction factors for gallic acid and protocatechuic acid, and use the correction factors to calculate gallic acid, The content of protocatechuic acid, realizes the quality control of Radix Sinensis, specifically comprises the following steps:

[0057] Step 1, preparation of reference substance standard solution

[0058] Step 1.1. Accurately weigh 0.0025g of gallic acid standard substance, 0.0104g of resveratrol standard substance, and 0.0103g of protocatechuic acid standard substance into 25ml volumetric flasks, and dilute with methanol to prepare a concentration of 0.100mg The gallic acid standard solution per ml, the resveratrol standard solution with a concentration of 0.416mg / ml and the protocatechuic acid standard solution with a solubility of 0.412mg / ml a...

Embodiment 2

[0098] The method of evaluating the quality of Baishui with one test and multiple evaluations adopts the method of one test and multiple evaluations, and uses resveratrol as an internal reference to establish relative correction factors for gallic acid and protocatechuic acid, and use the correction factors to calculate gallic acid, The content of protocatechuic acid, realizes the quality control of Radix Sinensis, specifically comprises the following steps:

[0099] Step 1, preparation of reference substance solution

[0100] Step 1.1. Accurately weigh 0.0025g of gallic acid standard substance, 0.0104g of resveratrol standard substance, and 0.0103g of protocatechuic acid standard substance into 25ml volumetric flasks, and dilute with methanol to prepare a concentration of 0.100mg The gallic acid standard solution per ml, the resveratrol standard solution with a concentration of 0.416mg / ml and the protocatechuic acid standard solution with a solubility of 0.412mg / ml are for su...

Embodiment 3

[0129] Using the method of one test and multiple evaluations, using resveratrol as an internal reference, the relative correction factors of gallic acid and protocatechuic acid were respectively established, and the contents of gallic acid and protocatechuic acid were calculated using the correction factors, so as to realize the quality control, including the following steps:

[0130] Step 1, preparation of reference substance solution

[0131] Step 1.1. Accurately weigh 0.0025g of gallic acid standard substance, 0.0104g of resveratrol standard substance, and 0.0103g of protocatechuic acid standard substance into 25ml volumetric flasks, and dilute with methanol to prepare a concentration of 0.100mg The gallic acid standard solution per ml, the resveratrol standard solution with a concentration of 0.416mg / ml and the protocatechuic acid standard solution with a solubility of 0.412mg / ml are for subsequent use;

[0132] Step 1.2, accurately measure 4ml of the above-mentioned gall...

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Abstract

The invention provides a method for evaluating quality of Ampelopsis japonic(Thunb.)Makino through quantitative analysis of multicomponents by a single marker. According to the method for evaluating the quality of the Ampelopsis japonic(Thunb.)Makino through the quantitative analysis of multicomponents by the single marker, firstly, a HPLC content determination method for three main medicinal components in the Ampelopsis japonic(Thunb.)Makino is established; resveratrol is used as an internal reference substance, relative correction factors of another two active components are calculated, andthe system suitability and the method reproducibility of the relative correction factors are investigated; chromatographic peak positioning is carried out according to relative retention time, the relative correction factors are combined for calculating the content of all to-be-detected components, through the mutual verification of the quantitative analysis of multicomponents by the single markerand an external standard method, it is proved that no significant difference exists in determination results; the problems that reference substances are high in costs and are not easy to obtain are solved, the content of index components is calculated through the relative correction factors and the chromatographic peak positioning, and the simultaneous determination of multiple medicinal substances in the Ampelopsis japonic(Thunb.)Makino is realized; and the costs can be saved, the operation can be simplified, the efficiency is improved, the detection sensitivity is high, the stability is good, the determination results are exact and reliable, and the method has great significance for ensuring the quality control and the clinical effects of the Ampelopsis japonic(Thunb.)Makino.

Description

technical field [0001] The invention belongs to the technical field of drug quality control, and in particular relates to a method for evaluating the quality of white scallops by a one-test and multiple-evaluation method. Background technique [0002] Ampelopsis japonica (Thunb.) Makino is a plant of the genus Ampelopsis japonica (Thunb.) Makino in the grape family. Boils, burns and other symptoms. The contents of gallic acid, protocatechuic acid and resveratrol in the medicinal materials of Radix Pachyrhiza are relatively large. Gallic acid has anti-inflammatory, antibacterial, antiviral, and antioxidative effects, and it is beneficial to the cardiovascular system, nervous system, diabetes, and liver fibrosis. It can prevent and treat diseases such as cancer and tumors; protocatechuic acid has protective effects on myocardial ischemia / reperfusion injury in rats, and has antibacterial and anti-arteriosclerosis effects; resveratrol can reduce myocardial ischemia-reperfusion ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 张寒郑梦迪张彦张剑柯云
Owner XIAN MEDICAL UNIV
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