38 results about "Pepsin digestion" patented technology

A method is described to provide composition for a nutritional supplement for convalescing patients recovering from illness or surgery, those with limited appetite such as the elderly, children or anorexic patients, or those who have impaired ability to digest other sources of protein such as persons having chronic gastritis who have a reduced gastric pepsin digestion. The supplement comprises: (i) a protein source which provides at least about 8% total calories of the composition and which includes at least about 50% by weight whey protein; (ii) a lipid source having an omega 3:6 fatty acid ratio of about 5:1 to about 10:1 and which provides at least about 18% total calories of the composition; (iii) a carbohydrate source; and (iv) a balanced macronutrient profile comprising at least vitamin E and vitamin C. The supplement has reduced capacity to induce satiety. Also disclosed is a method of treatment which comprises administering an effective amount of the composition.

The invention relates to a preparation method of feather albumen powder and the feather albumen powder prepared by the method. The method comprises the following steps: firstly carrying out pretreatment of washing, drying and cutting; then effectively carrying out steam explosion on feather by a steam explosion technique to damage the stable space structure of feather keratin; and finally carryingout zymohydrolysis treatment to enable the feather to become dissoluble small peptide and amino acid, thereby enhancing the digesting rate of pepsin of feather albumen and largely enhancing the biologic valence. The preparation method has the advantages of simplicity, low production cost, short processing period and high equipment efficiency. The content of crude albumen in the feather powder achieves more than 90%, and the digesting rate of in vitro pepsin achieves more than 88%.

A type IV collagen high molecular form having a higher molecular weight than the 7S domain of type IV collagen and including the 7S domain in its structure, is obtained from the supernatant being recovered from a collagen solution digested by pepsin in the following steps; 1) salt precipitating with sodium chloride at a concentration no higher than 1.2 M, 2) dissolving the precipitates, 3) salt precipitating with sodium chloride at a concentration no higher than previous concentration. By reacting a sample with an antibody which reacts with this form, the type IV collagen high molecular form in the sample can be measured to allow diagnosis of the degree of liver fibrosis in patients with liver diseases.

The invention discloses a method for making medical II collagen, which is characterized in: doing pre-treatment by using fresh articular cartilage as raw material; then using hydrochloric acid carbamidine to extract proteoglycan which comprises preparing a 4M hydrochloric acid carbamidine solution, and regulating the pH value to between 6.5 and 7.5 with alkali, then pouring the Hydrochloric acid carbamidine solution which is 8 to 15 times of the articular cartilage plasma into the cartilage plasma and blend, placing the solution on the shaking bed, shaking for one or two days, centrifuging and obtaining a solid matter, removing residue hydrochloric acid carbamidine solution by ultra-pure water washing for several times; pepsin digestion, which comprises that 2000 to 5000 portions of 0.5 to 0.6M acetic acid solution is added in the solid matter, then 0.1 to 0.3 portions of 2000 to 5000 mass percent solid matter which is pepsin is added in twice , for the first time half of the total amount is added and shaken in the shaking bed for 24 hours ,for the second time the other half is added and shaken for 24 hours, centrifuged, and the supernatant is removed, added in 0.01 to 0.02M aqueous solution of EDTA and 1 to 4 inactivate pepsin; salted out with NaCl; dialyzed with Na2 HPO4 solution, then medical-grade II Collagen is obtained.

The invention relates to a biological modified producing peptide protein feed, which uses animal blood meal as a main raw material and uses beer grains as an auxiliary material, and is characterized in that the content of protein is 50-70 percent, the amino acid is equal or greater than 40 percent, and the true nutritive value of pepsin is equal or greater than 90 percent. The invention further comprises massive vitamin, minerals, probiotics and enzyme. The product of the invention is used for culture industries such as livestock and poultry, and has the functions of rapid proliferation and reducing feed conversion ratio and the like. The method for preparation is that using the process of combining mixed solid fermentation and adding exogenous compound enzyme, big molecular matters of the blood meal and the beer grains are biologically modified into small molecular protein, polypeptide and free amino acids and the like, and then being dried, crushed and packaged. The invention has the characteristics of simple process, easy operation, short production cycle, low production cost, and excellent product quality.

The present invention is directed to a pharmaceutical composition comprising F(ab′)₂ antibody fragments that are preferably free from albumin and of whole antibodies and also substantially free of pyrogens, and an effective amount of a pharmaceutically acceptable carrier. It is also directed to a method for the production of a pharmaceutical composition comprising F(ab′)₂ antibody fragments using serum or blood plasma of a mammal that has been previously immunized as a source of antibodies. The serum or blood plasma is digested with an enzyme pepsin, followed by separation and purification until the pharmaceutical composition of F(ab′)₂ fragments is free of albumin and complete antibodies, and substantially free of pyrogens.

The invention discloses a hydrogel, and a preparation method and a three-dimensional cell culture method thereof. The preparation method of the hydrogel comprises the following steps: S1, preparing pig acellular dermis and/or pig acellular bladder; S2, freeze-drying the porcine acellular dermis and/or the porcine acellular bladder; S3, grinding the freeze-dried porcine acellular dermis and/or freeze-drying the porcine acellular bladder into fine particles; S4, adding the fine particles into pepsin digestive juice, and stirring for 48-72 hours to form an extracellular matrix scaffold; and S5, adjusting the pH value and the salt concentration of the pepsin digestive juice to inactivate the pepsin, and recombining the protein in the extracellular matrix scaffold to form the hydrogel.

The invention discloses a rapeseed protein and a preparation method thereof and relates to plant protein. The rapeseed protein comprises the following components in percentage by mass: 56.8-62.5% of crude protein (N*6.25, dry basis), 10-12% of water, 0.5-1.0% of polyphenol, 0.8-1.0% of phytic acid, 6-10% of crude fiber and 6-10% of crude ash as well as 0.6-1.2 micromoles glucosinolate per gram of rapeseed protein, wherein the digestibility of the rapeseed protein digested by pepsase is 85-95%. The rapeseed protein disclosed by the invention has the advantages of no addition of any organic solvent and acid-base fluid, mild reaction condition, simple process, easiness in operation, lower production cost, no pollution to environment, stable quality of the obtained rapeseed protein and easiness in industrialization.

The invention discloses a preparation method and application of a temperature-sensitive antler cartilage matrix hydrogel material, belongs to the technical field of tissue engineering, and aims to solve the technical problem of incomplete cell removal of an acellular matrix material for antler cartilage at present. The method comprises the following steps of removing blood and skin of pilose antler, soaking the pilose antler in a PBS buffer solution containing aprotinin, pressurizing the pilose antler in a closed pressurizing device, slicing and grinding the pilose antler, mixing the ground pilose antler and a freeze-thaw buffer solution in a metal closed container for incubation, quickly freezing a mixture with liquid nitrogen, unfreezing the mixture, and treating the mixture with trypsindigestive juice, an EDTA decontaminant, a nucleic acid remover and a bacterial remover in sequence, carrying out vacuum freeze drying, and performing digestion by pepsase digestive juice to obtain temperature-sensitive antler cartilage matrix hydrogel. Compared with an existing antler cartilage decellularization treatment method, the treatment method has a better cell removal effect, extracellular matrix components are not seriously lost through decellularization treatment, gaps of a matrix material obtained after decellularization are uniform, and the vascular lumen structure is still reserved.

The invention relates to a determination method for the digestion rate of pepsin and application of the determination method and belongs to the technical field of detection of chemical components. Themethod comprises the following steps: dividing the same sample into a first test sample and a second test sample; after degreasing the first test sample, determining the content of crude protein; degreasing the second test sample, carrying out enzymolysis on the pepsin and centrifuging; after washing, obtaining residues; determining the content of the crude protein in the residues. The digestionrate of the pepsin is calculated according to the following formula: the digestion rate is equal to (the content of the crude protein in the first test sample-the content of the crude protein in the second test sample)/the content of the crude protein in the first test sample*100 percent. The content of the crude protein is measured in percentage by mass. The determination method is efficient andconvenient to operate; the variety of used reagents is few and the source is wide; a result determined by the determination method has good repeatability and is accurate and effective. The method is used for determining the digestion rate of the pepsin and can realize batch treatment; the efficiency is improved and the cost is saved.