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A kind of joint preparation method of polysaccharide and protein peptide

A combined preparation and protein peptide technology, which is applied in the separation and extraction of proteins and the field of polysaccharides, can solve the problems of lack of joint preparation process, low utilization rate of raw materials, low purity of Bao polysaccharides, etc.

Inactive Publication Date: 2018-01-19
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the process of using abalone viscera to prepare polysaccharides and protein peptides is mainly to extract and prepare polysaccharides or protein peptides separately, lacking a joint preparation process, and the utilization rate of raw materials is low
At the same time, since the polysaccharides and proteins in abalone viscera mainly exist in the form of glycoproteins, the abalone polysaccharides prepared by hot water / alkaline extraction, single enzymatic hydrolysis or membrane separation technology all contain more protein components. As a result, the purity of Bao polysaccharide is low, and whether the Sevag method or trichloroacetic acid method is used for deproteinization, or hydrogen peroxide or activated carbon is used for decolorization, the yield of the product will be significantly reduced.

Method used

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  • A kind of joint preparation method of polysaccharide and protein peptide
  • A kind of joint preparation method of polysaccharide and protein peptide
  • A kind of joint preparation method of polysaccharide and protein peptide

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Experimental program
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Embodiment 1

[0029] A method for the joint preparation of polysaccharides and protein peptides, comprising the following steps:

[0030] (1) Raw material pretreatment: Take 1.5kg of frozen abalone viscera, grind it through a 1mm aperture meat grinder, and send it to the enzymatic hydrolysis tank as raw material, add 7.5kg of water to fully mix to make a feed liquid, and heat the feed liquid to 105°C After 15 minutes, cool down to 50°C.

[0031] (2) The first enzymatic hydrolysis: adjust the pH of the feed solution to 10.0, the temperature is 52°C, add 600U / g of raw material alkaline protease, enzymolyze at a constant temperature for 8 hours, boil for 15min, cool to 30°C, and the enzymolyzate is at a speed of 5000rpm Centrifuge down to get the precipitate and enzymatic solution;

[0032] (3) Membrane separation for the first time: the enzymatic hydrolysis solution is separated by an ultrafiltration membrane with a molecular weight cut-off of 10000Da, and the ultrafiltration is stopped when...

Embodiment 2

[0037] A method for the joint preparation of polysaccharides and protein peptides, comprising the following steps:

[0038] (1) Raw material pretreatment: Take 1 kg of fresh abalone viscera, grind it through a 2mm aperture meat grinder, and send it into the enzymolysis tank as raw material, add 8 kg of water to fully mix to make a feed liquid, heat the feed liquid to 100°C for 10 minutes, and cool down to 50°C.

[0039] (2) The first enzymatic hydrolysis: adjust the pH of the feed solution to 10.0, the temperature is 52°C, add 200U / g of raw material alkaline protease, enzymolyze at a constant temperature for 16 hours, boil for 10min, cool to 30°C, and the enzymolyzate is at a speed of 5000rpm Centrifuge down to get the precipitate and enzymatic solution;

[0040] (3) Membrane separation for the first time: the enzymolysis solution is separated by ultrafiltration with a molecular weight cut-off of 5000Da, and the ultrafiltration is stopped when the volume of the retentate is a...

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Abstract

A joint preparation method of polysaccharides and protein peptides. The wet material of abalone viscera is minced, mixed with water and boiled, and the first enzymolysis is carried out with alkaline protease. Carry out the second enzymatic hydrolysis with protease, and then carry out membrane separation. The obtained retentate is freeze-dried, spray-dried or ethanol-precipitated, and vacuum-dried to obtain abalone viscera polysaccharides. The retentate is combined with the permeate obtained from the first enzymatic hydrolysis. After reverse osmosis or vacuum concentration treatment, freeze-dry or spray-dry to obtain abalone visceral protein peptide. The method of the invention is simple and practical in operation, can simultaneously prepare polysaccharides and protein peptides, and has high production efficiency.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction of polysaccharides and proteins, and in particular relates to a combined preparation method of polysaccharides and protein peptides. Background technique [0002] Fujian is a big province of abalone breeding. The annual output of abalone is nearly 100,000 tons, accounting for more than 80% of the total domestic output. With the rapid growth of market demand, the processing of dried and frozen abalone is developing rapidly. By-products such as internal organs weighing 1 / 4 are mainly used to produce low-value-added products such as feed at home and abroad. Most of the internal organs of abalone are not fully utilized, which not only wastes resources, but also causes environmental damage. pollute. Polysaccharides and proteins in the viscera of abalone account for about 20% and 50% of its dry matter, and it is an excellent resource for processing biological polysaccharides and small...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C08B37/00
Inventor 熊何健马英刘智禹何传波魏好程吴国宏王娇
Owner JIMEI UNIV
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