Injectable hydrogel prepared by mixing cartilage decellularization epimatrix with decalcified bone matrix and preparation method of injectable hydrogel

Abstract

The invention discloses injectable hydrogel prepared by mixing cartilage decellularization epimatrix with decalcified bone matrix and a preparation method of the injectable hydrogel. The preparation method comprises the steps that a homologous or heterogeneous cartilage is subjected to decellularization treatment to prepare the cartilage cell epimatrix and subjected to degreasing treatment, decalcifying treatment and decellularization treatment to prepare the decalcified bone matrix, the cartilage cell epimatrix and the decalcified bone matrix are mixed according to a certain proportion and then digested by pepsase, and the mixture is solidified into the injectable hydrogel at neutral pH at 37 DEG C under the suitable salt concentration condition. The injectable hydrogel which contains the mixed cartilage decellularization epimatrix and decalcified bone matrix and is prepared through the method has the good biodegradability and has the injectability above all; meanwhile, due to the fact that decellularization treatment is conducted, the immunogenicity is greatly reduced, the good biocompatibility is achieved, and biological rejection response is reduced. The injectable hydrogel has the advantages that the materials are easy to take, operation is convenient, the injectable hydrogel can be applied to various cartilage defect filling and minimally-invasive orthopaedic cartilage repairing treatment, and a good clinical application prospect is achieved.

Description

technical field [0001] The invention relates to biological tissue engineering technology, in particular to an injectable cartilage decellularized extracellular matrix mixed decalcified bone matrix hydrogel and a preparation method thereof. Background technique [0002] Clinically, articular cartilage damage and defect caused by degeneration, trauma, various inflammations, tumors and other factors have always been a clinical problem in orthopedics. The vast majority of articular cartilage is hyaline cartilage, without blood supply, lymphatic tissue and innervation, self-repairing Limited capacity and poor self-regeneration. The treatment of articular cartilage injury has become one of the most difficult problems in bone and joint surgery. [0003] Every year, millions of patients are treated clinically for articular cartilage damage. At present, both autologous cartilage transplantation and allogeneic cartilage transplantation have certain limitations. Autologous cartilage ...

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Problems solved by technology

However, simply transplanting chondrocytes, the cells cannot grow and proliferate, resulting in poor results.

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