50 results about "Hyaluronidase activity" patented technology

The problem of the present invention is to provide a cosmetic composition, which has a moisturizing effect, and whose ability to inhibit hyaluronidase activity is high. According to the present invention, by using one or more substances selected from sulfated cellulose and a salt thereof in combination with polyhydric alcohol, the moisturizing effect of sulfated cellulose and the ability to inhibit hyaluronidase activity can be further improved, and thereby the above-described problem can be solved. In addition, the present invention can provide a cosmetic composition having excellent stability through inclusion of an electrolyte having pH buffering ability.

The invention discloses a moisturizing composition with an anti-allergy repairing efficacy and preparation and application to skincare products. The composition is prepared from xanthan gum, pullulan,polyglutamic acid, hyaluronic acid, trehalose, allantoin, urea and chondroitin sulfate by reasonable compounding. The composition has a function of stereo moisturizing of all skin layers including acorium layer, an epidermal layer, a cuticle layer and a skin surface, the skin moisture content can be increased, and skin moisture loss is reduced. The composition is capable of inhibiting hyaluronidase activity, soothing skin allergy and pruritus and repairing skin barrier. The composition has excellent moisturizing effects and an anti-allergy repairing efficacy in vitro and on human skin, effects are remarkably improved as compared with those of single use of each component, and accordingly synergistic effects are achieved by combination of the components in an appropriate ratio. The composition is solid powder and free of any preservatives, and convenience in long-time storage and transport is achieved. The composition can be applied to all types of skincare and cleansing products.

The invention discloses a composition with anti-allergy and relieving effects and a preparation method and application thereof. The composition with anti-allergy and relieving effects has the functions of reducing allergic source irritation, inhibiting histamine release and hyaluronidase activity and repairing sensitive skin injured barriers. The composite is characterized by being mainly prepared from, by weight, 10-20% of plant extracts, 5-10% of trehalose, 20-30% of 1,3-propylene glycol and the balance water. The plant extracts mainly comprise, by weight, 10-15 parts of mirabilis jalapa, 10-15 parts of radix ophiopogonis, 10-15 parts of sophora flavescens and 5-10 parts of cactus. The composition belongs to the technical field of cosmetics.

The invention relates to a preparation method of a cross-linked sodium hyaluronate gel having a hyaluronidase activity inhibiting effect. The method utilizes a fact that different groups in sodium hyaluronate molecules are activated under different pH conditions, then different pH conditions are controlled, a hyaluronidase inhibitor is introduced into the sodium hyaluronate molecules, and finally the cross-linked sodium hyaluronate gel having the hyaluronidase activity inhibiting effect is obtained. Compared with a gel prepared by a traditional process, the cross-linked sodium hyaluronate gel prepared by the method has the advantages of low viscosity, long in-vivo residual time and the like, also can inhibit the hyaluronidase activity, and has the highest inhibition rate up to 65%. The low viscosity of the cross-linked sodium hyaluronate gel prepared by the method ensures convenience of clinical use, and the cross-linked sodium hyaluronate gel inhibits the hyaluronidase activity, significantly increases the in-vivo residual time, and has good application prospects in the aspects of cosmetic injections, artificial vitreous bodies and degenerative arthritis supplementary treatment.

The invention discloses a (Bacillus) A50 CGMCC No. 5744 and a method of producing hyaluronidase by employing the same. The method is that slant cultivation, seed cultivation and fermentation cultivation are carried out for the (Bacillus) A50 CGMCC No. 5744 so that hyaluronidase is prepared. The hyaluronidase generated by the Bacillus is highly active, up to 105IU/m1. As compared with the prior art, the hyaluronidase prepared from the Bacillus of the invention is high in activity, high in thermal stability and high in pH stability, and can be used for large scale production. Meanwhile, the obtained hyaluronidase can allow high-molecular hyaluronic acid to be degraded, can be used for preparing low-molecular hyaluronic acid and oligomeric hyaluronic acid, enables the cost to be substantially reduced, helps to solve the problem of high cost for hyaluronidase of animal sources, and exhibits a wide application prospect in the field of biochemistry research and for the production of low-molecular hyaluronic acid and oligomeric hyaluronic acid.

The invention discloses gentian compound extract. The gentian compound extract is prepared from 10-25 parts by weight of gentian root, 5-20 parts by weight of radix scutellariae, 3-10 parts by weightof centella asiatica, 1-8 parts by weight of radix sophorae flavescentis, 1-5 parts by weight of licorice root, 1-5 parts by weight of a plant extract enzyme, 10-30 parts by weight of low molecular weight alcohol and 40-80 parts by weight of deionized water. The invention also provides a preparation method of the gentian compound extract. The preparation method comprises: raw material crushing, temperature difference method extraction, enzymatic hydrolysis extraction, enzyme inactivation treatment and extraction liquid filtration. The contents of the five raw materials and other components arecontrolled in the optimal range and have reasonable compatibility so that the inhibition rate of hyaluronidase activity can reach to 76%. The gentian compound extract can fast resist allergy and relieve itching to reduce patient's pain. The wall is broken through a temperature difference-enzymatic hydrolysis method so that the gentiopicroside content is 2180.5ppm and thus the extraction rate of the active ingredients is greatly improved, the problem that the traditional method easily causes viscous substance blocking in the filter in extraction is solved, the energy consumption and equipmentrequirements are reduced and large-scale production is feasible.

The invention discloses a glycyrrhiza glabra extract and a preparation method and applications thereof. The method comprises the following steps: (1) pulverizing fresh glycyrrhiza glabra with a colloid mill to obtain a homogenate pulverized product; and (2) adding an extraction solvent to the homogenate pulverized product obtained in the step (1), performing ultrasonic extraction to obtain an ultrasonic extractive liquid, and then subjecting the ultrasonic extractive liquid to separation and concentration so as to obtain the glycyrrhiza glabra extract. The extraction solvent is an aqueous solution of butanediol or butanediol. The method of the invention is simple in process operation and is easy to realize industrial production. The obtained glycyrrhiza glabra extract contains glycyrrhizaflavonoids, glycyrrhizin and glycyrrhiza polysaccharide, is rich in active ingredients in fresh plants, has obvious inhibition on tyrosinase activity and inhibition on hyaluronidase activity, and is suitable for whitening, anti-inflammatory and anti-allergic cosmetics.

The invention relates to aloe flower extract and a preparation method thereof and in particular relates to moisturizing, anti-inflammatory, anti-oxidization and whitening aloe flower extract and a preparation method thereof. The aloe flower extract prepared by the preparation method is subjected to experiments including hyaluronidase activity inhibition, DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical inhibition, reduction capability, tyrosinase activity inhibition and the like to prove the effects in moisturizing, repairing, anti-oxidization and whitening aspects; the aloe flower extract can be used for preparing moisturizing, anti-allergy, whitening and anti-ageing cosmetics and has the effects of increasing the hydration of skin and reducing irritation of external environment on skin,reducing injuries of free radicals on the skin, inhibiting the generation of melanin and helping the skin to recover a healthy state.

The invention provides a new method for preparing 1, 2, 3, 4, 6-five-O-galloyl-Beta-D- glucose (PGG) by using tannin and the new use thereof. The tannin is hydrolyzed by acid to prepare a PGG crude product, and chromatographic separation and purification are then carried out on the PGG crude product by macroporous absorbent resin and hyskon gel columns to obtain PGG with the purity quotient more than 90 percent. The PGG prepared by the method has good colour and lustre, high purity quotient, simple, safe and easy preparation process, low cost, short production period and easy batch preparation and commercial process. The activity rating shows that the inhibited effect (IC50 0.01mM) of the PGG for the activity of hyaluronate lyase is obviously higher than that of positive control cromolyn sodium (IC50 6.99mM), and the PGG can be directly used as a raw material or the additive of medicine, health food and cosmetics.

The present invention provides an immune cell expressing hyaluronidase and an application thereof. The immune cell comprises a fusion gene and the fusion gene mainly comprises a signal peptide, a hyaluronic acid catalytic active region, and a stable sequence in series. The signal peptide is the signal peptide of secreted proteins. The provided immune cell expressing the hyaluronidase can express and secrete the hyaluronidase as a protein, the hyaluronidase is secreted outside the cell to degrade extracellular matrix, in a natural state, half-life of the hyaluronidase is relatively short, so that hyaluronidase active sequence is fused with a signal peptide sequence and the stable sequence, which can extend the half-life of the hyaluronidase in body, promotes infiltration of the immune cellto specific tissues and thus increases functional activity of the immune cell against solid tumors.

The invention relates to Streptomyces koganeiensis ATCC 31394 hyaluronidase having molecular weight of 21.6 kDalton, which has hyaluronidase activity and stability markedly higher than those of the hyaluronidase obtained from such microorganism to date. The invention further relates to a process for the isolation and purification of said hyaluronidase and its use for the preparation of pharmaceutical compositions or as an analytical reagent.

The invention provides a novel method for preparing 1,2,3,4,6-penta-O-galloyl-beta-D-glucose (PGG) by tannin, which comprises the following steps of: hydrolyzing tannin with acid to obtain a PGG rough product; and separating and purifying by using macroporous absorption resin and glucan gel column chromatography to obtain the PGG with the purity of larger than 90 percent. The method has the advantages of good color and high purity of the obtained PGG, simple, safe and easy preparation process, lower cost and short production period and is easy for mass preparation and industrialized production. Indicated by activity evaluation, the inhibiting action (IC50 0,01mM) of the PGG on hyaluronidase activity is obviously higher than that of positive control sodium cromoglycate (IC50 6.99mM), and the PGG can be directly used as a raw material of a medicament, a heat care food and cosmetics or additive thereof.

The present invention discloses a method for rapid determination of hyaluronidase activity, and the method comprises the following steps: (1) using a sodium acetate-acetic acid buffer solution for preparation of a hyaluronic acid solution with the pH value greater than 3 and less than or equal to 7; (2) preparing a cetyl trimethyl ammonium bromide solution; (3) taking the hyaluronic acid solution of the step (1), adding a hyaluronidase solution for reacting for 0.5 to 1 hour at 37 DEG C, and adding the cetyl trimethyl ammonium bromide solution for reacting at 37 DEG C for 8 to 15 minutes to obtain a sample solution; (4) preparing a control solution, to be more specific, taking the hyaluronic acid solution of the step (1), and adding the cetyl trimethyl ammonium bromide solution for reacting at 37 DEG C for 8 to 15 minutes to obtain a control solution; (5) using a microplate reader to determine optical density values of the sample solution and the control solution at a wavelength of 400nm; and (6) according to the obtained optical density values of the sample solution and the control solution, calculating hyaluronidase activity and specific activity. The method is simple to operate, good in stability and high in sensitivity and reproducibility, and lays a foundation for scale extraction, preparation and application of hyaluronidase.

The invention relates to a solution with anti-oxidization, restoration, anti-winkle and water supplementation functions and a preparation method thereof. The solution is prepared from hydrolyzed sodium hyaluronate, sodium polyglutamate, phenoxyethanol and water. The solution provided by the invention has the advantages that the cooperated enhancing effect of all ingredients is achieved; the long effect moisture preservation is realized; the enzymatic activity of hyaluronic acid is inhibited; the natural moisture preservation factors are generated; the skin elasticity is maintained; the monophenyl oxidase activity is mildly inhibited; the melanin generation is inhibited; the oxidization and pigmentation can be resisted; the deep layer moisture preservation is realized; the luster whiteningeffect is achieved. The skin feeling of the solution is smooth but not greasy.

Porphyran with modified bioactivity and physical property, method of making the modified Porphyran and use of modified Porphyran. Porphyran with modified bioactivity and physical property is produced by dissociating a salt from a sulphate group in Poryphran and removing it, making Porphyran having a free sulphate group wherein a salt is dissociated. Further, Porphyran with modified bioactivity and physical property is produced by dissociating a salt from sulphate group in Porphyran and removing it, and then altering the sulphate group, with the use of a given salt, into a sulphate salt of the given salt. The modified Porphyran also can be obtained by converting a salt of a sulphate group in Porphyran into a sulphate salt of a given salt by ion exchange. The modified Porphyran be added to cosmetics, food, and drink, and used as cosmetic, food and drink having inhibitory activity against hyaluronidase activity.

The invention provides a composition for inhibiting enzyme activity of hyaluronic acid. The composition comprises glutamic acid, theanine and mannose. The invention also provides a skin care product.The skin care product is characterized by comprising the composition for inhibiting enzyme activity of hyaluronic acid and the hyaluronic acid. The composition capable of inhibiting enzyme activity ofhyaluronic acid provided by the invention has an obvious inhibiting effect on the enzyme activity of hyaluronic acid, the enzyme activity of the hyaluronic acid can be effectively inhibited by the composition with low addition quantity, and the half inhibition dosage (IC50) for the enzyme activity of the hyaluronic acid can be as low as 0.17 mg/mL. The stability of a hyaluronic acid solution canalso be improved, and the degradation speed of the hyaluronic acid in a solution state is reduced. According to the skin care product provided by the invention, the composition for inhibiting the enzyme activity of hyaluronic acid can promote the exertion of the efficacy of the hyaluronic acid and improve the moisturizing effect of the hyaluronic acid.

The invention discloses a research method for a screening and extracting process of chamomile anti-asthma active ingredients, and relates to the technical field of chamomile processing. The method comprises the following steps: S01, carrying out clustering analysis on chamomile medicinal materials in different batches, and determining a suitable production place of chamomile; S02, determining optimal extraction conditions of a chamomile active part extraction process by taking dicaffeoylquinic acid content, extract yield and hyaluronidase activity as indexes; and S03, taking the two characteristic components of 3, 5-di-O-caffeoylquinic acid and 4, 5-di-O-caffeoylquinic acid as indexes, purifying the two characteristic components, and determining the optimal purification conditions of the purification process of the chamomile active site. Through the systematic analysis method, the anti-asthma active components in the chamomile are systematically extracted and produced, and through an optimal solution of an extraction and purification process of the anti-asthma active components, the extraction efficiency of the anti-asthma active components in the chamomile is improved, and the production efficiency is improved.