53 results about "Gene induction" patented technology

Methods and compositions relating to TIC10 are described according to aspects of the present invention. The compositions and methods have utility in treating disease, particularly cancer in a subject in need thereof, including a human subject as well as subjects of other species. The compositions have utility in treating brain cancer in a subject in need thereof.

The invention discloses a method for breeding maize haploid inducer lines. By developing a method for identifying colored embryo haploids of the inducer lines, the haploid identification of the maize haploid inducer lines can be realized, thereby doubling, and The induction line DH line with high induction rate, high identification efficiency and excellent agronomic traits can be screened from four aspects of molecular marker-assisted selection: induction gene, induction rate, haploid identification efficiency and agronomic traits. The overall process takes 1 ‑2 years to obtain an excellent haploid induction line. The method created by the present invention, which can be used for rapid selection of haploid induction lines in maize, accelerates the breeding process of haploid induction lines, and provides a solution to the problems of long breeding cycle and heavy workload of haploid induction lines in maize. A feasible plan can greatly shorten the breeding period of haploid induction lines, reduce the testing workload of each generation, improve the efficiency of haploid breeding, and thus promote the development of maize haploid breeding technology, which is of great significance.

The invention relates to an accelerator and a preparation method thereof, belonging to the technical field of animal husbandry biological products; by weight percentage, it comprises: 82% corn steep liquor, 6% urea, 5% mannitol, 4% DL-methionine, and L-arginine 3%; a kind of accelerant of 5-aminolevulinic acid comprises by weight percentage: dipotassium hydrogen phosphate 2.5%, L-cysteine ​​hydrochloride 9%, L-alanine 84.5%, sodium chloride 4 %; B. mixed and stirred in a dry powder mixer for 60 minutes to obtain the finished product; a kind of accelerator of 5-aminolevulinic acid comprises by weight percentage: lactose 12%, triammonium citrate 3.5%, L-asparagine 14%, hemoglobin 1.5%, L-serine 69%; the accelerator provided by the invention can increase the content of 5-aminolevulinic acid in the photosynthetic bacteria fermentation broth, and has the advantages of simple production process, easy operation, no transgene and gene induction It has the advantages of low energy consumption, easy access to raw materials, high yield, low cost, convenient use, and more obvious effects.

The invention provides an expression method of recombinant human insulin, a special expression vector, engineering bacteria and application. This expression method can induce the expression of recombinant human insulin gene in food-grade lactic acid bacteria and display recombinant human insulin on the surface of lactic acid bacteria. The engineering bacteria, expression vector and inducer used in this expression method all meet the requirements of food grade, avoiding the The presence of resistance genes on vectors and the potential harm caused by non-food-grade inducers, etc. The lactic acid bacteria engineering bacteria containing cell wall display insulin obtained by the present invention can stimulate NOD mice to produce recombinant human insulin-specific antibodies, significantly increase the level of cytokine IL-4 related to immune tolerance, and induce immune tolerance. The induced lactic acid bacteria engineered bacteria can be used as an oral vaccine for type 1 diabetes after being made into bacterial preparations, without the need for tedious post-processing such as purification, and has broad application prospects.

Visual self-sucking method for inducing external gene into insect eggs is carried out by: under telescope, coating solution with external genes on insect eggs, pricking eggs to make external gene into eggs, making external genes combined with insect's gene to form transgenic insects. It makes technicians to do researches on transgenic insects with cheap instrument.

The present invention relates to the protective effect and application of FSTL1 in the homeostasis regulation of tissues such as liver against fibrosis, in particular to the application of FSTL1 protein or FSTL1 regulating compound in the preparation of medicine for treating tissue fibrosis. The invention also relates to a conditional FSTL1 gene knockout non-human animal model closely related to the degree of tissue fibrosis and its preparation method, and its use in screening anti-tissue fibrosis drugs. Among them, by combining different doses of tamoxifen and different stages of animal growth, a non-human animal model of systemic or vascular endothelial cell FSTL1 gene-induced knockout is obtained to simulate different tissue fibrosis lesions.