33 results about "DNA protection" patented technology

The preparation of extracts from olive fruits, olive tree leaves, olive oil as well as olive-press waste. The isolation of natural products from these extracts and the evaluation of the DNA protective antioxidant activity of the extracts and the purified compounds on intact cells.

The invention discloses a compound with the functions of radiation prevention, oxidation resistance and DNA protection. The invention is prepared by sea protein peptide and ganoderma lucidum polysaccharide with the weight ratio of 18 to 1 and has the functions of preparing radiation prevention drugs, radiation prevention health foods and radiation prevention foods. The preparation of the invention has the features of easy absorption, rapid absorption, low viscosity and good water-solubility. The invention ensures the improving of the survival rate and average survival time of a radiating mouse 30d, the number of the peripheral blood leukocytes, the amount of DNA of myeloid cells, superoxide dismutase (SOD) of serum and the 50% hemolytic dose (HC50). Further, the invention has the effect of radiation prevention, and the radiation prevention effect of the invention is superior to the radiation prevention effect of the ganoderma lucidum polysaccharide when the ganoderma lucidum is provided solely. The employment of the invention on the radiation drugs, radiation health foods and radiation foods can solve the problem that great toxicity universally exists on the current mercapto compounds.

The invention relates to a method for purifying deoxyribonucleic acid (DNA) in a DNA bisulfate conversion process and belongs to the technical field of nucleic acid conversion and purification. According to the method disclosed by the invention, a DNA protecting agent capable of preventing DNA from fragmenting and degrading is added to a DNA bisulfate conversion system, so that the DNA is not fragmented or degraded in the treatment process of bisulfate. Thus, the quality of the processed DNA is effectively improved; the yield of nucleic acid is about 70%; and the sensitivity of polymerase chain reaction (PCR) and other analysis technologies is improved. By adopting the method disclosed by the invention, the entire treatment process of the DNA bisulfate is simpler and faster; and meanwhile, the quality of the processed DNA is improved.

The invention provides a silver nano-cluster fluorescent probe based on double-stranded DNA protection and application of the same to preparation of a drug used for detecting Plasmodium falciparum lactate dehydrogenase, belonging to the technical field of fluorescent probes. DNA used in the invention is of a double strand structure, wherein one strand is composed of a complementary strand DNA and a template strand DNA, and the other strand is composed of a complementary strand DNA and G base-rich DNA; the template strand DNA is a protective group for synthesis of a silver nano-cluster and can be coordinated with the surface of the silver nano-cluster to prevent further expansion of the silver nano-cluster; the G base-rich DNA improves the fluorescence emission intensity of the silver nano-cluster by approaching the silver nano-cluster; the complementary strand DNA has a length of 10 to 30 bases and is rich in A (adenine) and T (thymine) bases; the template strand DNA has a length of 10 to 20 bases and is rich in C (cytosine) bases; and the G base-rich DNA has a length of 10 to 25 bases and is rich in G bases. A detection method provided by the invention is fast in detection speed, simple to operate, simple in system, stable in signal, high in sensitivity and free of any pretreatment and does not need any complex detection apparatuses.

The invention provides a bacteriocin-producing paenibacillus ehimensis strain NPUST-1 which can promote the growth and immunoreaction of aquaculture organisms. At the same time, the strain can also improve the survival rate of aquaculture organisms after being infected with pathogenic bacteria. In addition, the bacteriocin Peocin produced by the paenibacillus ehimensis strain NPUST-1 is a DNA protective protein in the starvation / stagnation period, and has antibacterial activity against a variety of pathogens including culture pathogens, foodborne pathogens and clinical pathogens. The bacteriocin Peocin is found out to be the DNA protein in starvation / stagnation period and with antibacterial activity for the first time.

The invention discloses an improved method for converting a plant pollen tube. Aluminum hydroxide sol, calcium phosphate and Tween20 are adopted respectively to serve as exogenous plasmid DNA protective agents, and a plant pollen tube channel method is utilized to convert an exogenous gene. Receptor tobacco and soybean GUS active tissue histochemical staining results show that the gus positive rates with the aluminum hydroxide sol serving as the DNA protective agent are respectively 11.1% and 3.78%; the gus positive rates with calcium phosphate serving as the DNA protective agent are respectively 8.89% and 2.91%; the gus positive rates with Tween20 serving as the DNA protective agent are respectively 10.0% and 1.41 %; and the gus positive rates with 1*SSC solution serving as the DNA protective agent are respectively 7.78% and 1.97 %, the problem that the conversion rate in the plant pollen tube channel method is low is solved, and the conversion rate is improved.

The present invention relates to a functional health-care food with the functions of resisting radiation, resisting free radical and protecting DNA. Its preparation method includes the following steps: making ganoderma spore powder undergo the process of low-temp. antioxidative wall-breaking treatment; water-extracting or alcohol-extracting ganoderma sporocarp to obtain its coarse polysaccharide; using any one method described in the application specification to extract jinsiyi (a Chinese medicinal material) to obtain its polysaccharide; extracting tea to obtain tea-polyphenol; mixing the above-mentioned materials and making them into capsule or tablet preparation so as to obtain the invented product.

The preparation of extracts from olive fruits, olive tree leaves, olive oil as well as olive-press waste. The isolation of natural products from these extracts and the evaluation of the DNA protective antioxidant activity of the extracts and the purified compounds on intact cells.

The invention discloses collagen for skin repairing and a preparation method of the collagen. Sea cucumber is taken as a raw material and processed with an ultrahigh-pressure processing method and a protease enzymolysis method, meanwhile, an enzymatic hydrolysate is subjected to fishy smell removing treatment by a fishy smell removing agent, and therefore, the prepared collagen for skin repairingis high in active ingredient content, good in solubility, high in absorptivity and free of fishy smell, has multiple effects of oxidation resistance, inflammation resistance, DNA protective capability, moisturizing capability, anti-wrinkle property, skin whitening and the like and can be taken as the raw material to be applied to multiple fields of cosmetics, dietary supplements, food, medicine and the like.

The invention discloses a genome DNA (Deoxyribonucleic Acid) preservation solution of a saliva and oral cavity swab. The genome DNA preservation solution is characterized in that the preservation solution comprises the following components according to the concentration of 1 liter of the preservation liquor: 0.10 to 0.2 percent of SDS (sodium dodecyl sulfate), 0.90 percent of NaCl, 5 to 15mM of Tris(trihydroxymethylaminomethane), 20 to 25mM of EDTA (Ethylene Diamine Tetraacetic Acid) and 150 to 160mg of proteinase K. The preservation solution of the saliva and oral cavity swab takes the SDS,Tris-HCl, the EDTA, the NaCl and the proteinase K as main components and can be used for preserving saliva. The NaCl can be used for maintaining osmotic pressure of cells; an SDS surfactant can be used for damaging a protein component and inhibiting the breeding of bacteria under the action of the proteinase K and the degradation of DNA is easy to cause; and the Tris-HCl and the EDTA can be used for protecting free DNA. The DNA preservation solution provided by the invention is a preservation solution aiming at the preservation of the oral cavity swab and can be used for preserving oral cavitycells relatively well; the DNA preservation solution is more sanitary and the bacteria are not easy to breed; the preservation time is 3 to 5 times longer than the preservation time in an envelope; and a preparation method is simple and has relatively good social value.

The invention discloses an exopolysaccharide and application thereof, belonging to the technical field of bioengineering. The exopolysaccharide (EPS) is a heteropolysaccharide formed by glucosamine, arabinose, galactosamine, galactose, glucose and mannose secreted from clostridium butyricum (Clostridium Butyricum) with a preservation number of CCTCC NO:M 2018426 and has relatively high free radical scavenging capacity than ascorbic acid; a good DNA protection effect can be provided for oxidative stress induced by AAPH and hydroxyl free radicals; and meanwhile, the direct damage caused by ultraviolet radiation to the activity of DNA.

The invention relates to a Mangifera (Mango) Indica preparation as Sirtuin 1 activating agent for in vivo and in vitro applications. The preparation may be used to reduce the risk of developing obesity, type II diabetes, elevated blood lipid levels, artheriosclerosis and cardiovascular diseases, as well as a cell and DNA protector.

The invention discloses application of milkfish fish scale collagen protein which has the effects of oxidation resistance, inflammation resistance, DNA protection capability, moisture retention capability, wrinkle resistance function, skin whitening effect and the like and can be respectively applied to corresponding components in cosmetics.

The invention discloses a composition for DNA protection. More particularly, the invention discloses a composition to reduce DNA damage, to repair the damaged DNA and to enhance the DNA repair wherein such DNA damage may be caused due to alcohol consumption or due to any other known or unknown reasons.

The invention belongs to the technical field of gene engineering, and particularly relates to a preparation method and a transformation method for efficiently transforming competent cells from pichia pastoris, and the method comprises the following steps: inoculating pichia pastoris for a first culture, and then transferring for a second culture to obtain a first bacterial liquid; performing a third culture on the first bacterial liquid to obtain a second bacterial liquid; the second bacterial liquid is subjected to resuspension activation with a cell osmotic pressure protection liquid, a cell membrane permeability enhancement liquid, a cell stabilizing liquid and a cell DNA protection liquid in sequence, and competent cells are obtained. According to the competent celsl obtained by the method, the activity of the competent cells is 85%-95%, the competent cells have extremely high transformation efficiency, and 2*10 <3> transformants can be obtained by transforming the competent cells with plasmids (the concentration is 5[mu]g / [mu]l); the success rate of PCR identification reaches 90% or above.

The invention relates to a method for purifying deoxyribonucleic acid (DNA) in a DNA bisulfate conversion process and belongs to the technical field of nucleic acid conversion and purification. According to the method disclosed by the invention, a DNA protecting agent capable of preventing DNA from fragmenting and degrading is added to a DNA bisulfate conversion system, so that the DNA is not fragmented or degraded in the treatment process of bisulfate. Thus, the quality of the processed DNA is effectively improved; the yield of nucleic acid is about 70%; and the sensitivity of polymerase chain reaction (PCR) and other analysis technologies is improved. By adopting the method disclosed by the invention, the entire treatment process of the DNA bisulfate is simpler and faster; and meanwhile, the quality of the processed DNA is improved.

The invention discloses a method for rapidly and efficiently extracting genomic DNA of mammal ear tissue. The method comprises steps as follows: 1) tissue disruption and DNA protection; 2) tissue lysate digestion; 3) Tris-saturated phenol extraction; 4) Tris-saturated phenol and chloroform / isoamyl alcohol mixed extraction; 5) chloroform / isoamyl alcohol extraction; 6) precipitation of DNA with NaAc and fully precooled (subzero 20 DEG C) absolute ethanol; 7) ethanol rinsing; 8) dissolution of DNA with a TE buffer solution and preservation. The yield of DNA is significantly increased (the concentration of DNA is 112-851 ng / mu L), meanwhile, the time spent on addition of proteinase K for digestion overnight (longer than or equal to 12 h) after cell disruption during DNA extraction is saved, and about 3 h is spent from tissue disruption to genomic DNA extraction.

The invention provides a quality control product of a chromosome aneuploidy detection kit capable of being stably stored, and application. The quality control product comprises a positive reference product and a negative reference product, wherein the positive reference product contianins DNA protecting agents and DNA fragments from 150 to 200bp intervals with the nuclear type analysis result being 47 and TN1 human genome DNA amplification products; the negative reference product contains a DNA protecting agents and DNA fragments which are not from 150 to 200bp intervals with the nuclear typeanalysis result being 47 and TN1 human genome DNA amplification products; the TN1 is selected from one several kinds of materials from T21, T18 and T13; the DNA protection agents contain aurintricarboxylic acid ammonium salt, glycine and bestatin hydrochloride. The quality control product provided by the invention has the advantages that the protection agents are added into each reference product;the DNA degradation of the reference product in the quality control product is inhibited, so that the stability is higher; the storage is easy; the shelf life is prolonged.

The invention provides a quality control product and application of a stable chromosomal aneuploidy detection kit, including a positive reference product and a negative reference product; 1 DNA fragments and DNA protection agents in the 150-200bp interval of the amplification product of human genomic DNA; 1 DNA fragments and DNA protection agents in the 150‑200bp interval of the amplification product of human genomic DNA; the TN 1 One or more selected from T21, T18, T13; the DNA protection agent includes ammonium aurintricarboxylate, glycine, bestatin hydrochloride. The quality control product provided by the present invention inhibits the DNA degradation of the reference product in the quality control product by adding a protective agent to each reference product, making it more stable, easier to store, and prolonging the validity period.

The invention discloses an environment-friendly DNA preservation product, which is to sandwich DNA in a hard urea formaldehyde polymer resin containing a DNA protectant. A concrete production method comprises the steps: extracting the DNA; preparing urea formaldehyde monomers; synthesizing an urea formaldehyde polymer resin liquid containing the DNA protectant; pouring the synthesized urea formaldehyde polymer resin liquid containing the DNA protectant into a mold; compregnating the prepared DNA into the urea formaldehyde polymer resin liquid containing the DNA protectant; and drying the ureaformaldehyde polymer resin liquid containing the DNA protectant to be hardened, and demolding. The product has the advantages of simple production, low cost, energy consumption-free preservation, long preservation period and so on.

The invention discloses an active biological peptide nutrient solution. The nutrient solution is prepared from green and pollution-free raw materials, is natural, green, and environment-friendly, and does not have any side or toxic effect to skin. The nutrient solution can properly activate the proliferation of skin stem cells so as to accelerate the metabolism of skin cells, the skin becomes young, and the effect is prominent. Moreover, the nutrient solution can supply enough ATP energy to sustain the normal operation of skin cells, protects skin cells from oxides, maintains the skin water to ensure that the interstitial fluid of skin is sufficient, creates a pH neutral micro environment of sin, and guarantees that skin cells can play important physiological functions. The provided skincare product is a comprehensive and complete nutrient system, and can protect the DNA in skin cell nucleus, modulate the cell proliferation in the gene level, activate the cell metabolism functions, and enhances the energy supply of cells. The nutrient solution can maintain the stability of skin tissue structure.

The invention discloses an improved method for converting a plant pollen tube. Aluminum hydroxide sol, calcium phosphate and Tween20 are adopted respectively to serve as exogenous plasmid DNA protective agents, and a plant pollen tube channel method is utilized to convert an exogenous gene. Receptor tobacco and soybean GUS active tissue histochemical staining results show that the gus positive rates with the aluminum hydroxide sol serving as the DNA protective agent are respectively 11.1% and 3.78%; the gus positive rates with calcium phosphate serving as the DNA protective agent are respectively 8.89% and 2.91%; the gus positive rates with Tween20 serving as the DNA protective agent are respectively 10.0% and 1.41 %; and the gus positive rates with 1*SSC solution serving as the DNA protective agent are respectively 7.78% and 1.97 %, the problem that the conversion rate in the plant pollen tube channel method is low is solved, and the conversion rate is improved.

The invention discloses an environment-friendly DNA preservation product, which is to sandwich DNA in a hard urea formaldehyde polymer resin containing a DNA protectant. A concrete production method comprises the steps: extracting the DNA; preparing urea formaldehyde monomers; synthesizing an urea formaldehyde polymer resin liquid containing the DNA protectant; pouring the synthesized urea formaldehyde polymer resin liquid containing the DNA protectant into a mold; compregnating the prepared DNA into the urea formaldehyde polymer resin liquid containing the DNA protectant; and drying the urea formaldehyde polymer resin liquid containing the DNA protectant to be hardened, and demolding. The product has the advantages of simple production, low cost, energy consumption-free preservation, long preservation period and so on.