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Application of milkfish fish scale collagen protein

A fish scale collagen, milkfish technology, applied in the biological field, can solve the problems of very troublesome care, very sensitive constitution, etc., and achieve the effect of small loss of ammonia nitrogen

Inactive Publication Date: 2018-06-01
XIAMEN MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The scientific name of milkfish is Chanoschanos, and its English name is milkfish. It is a vegetarian fish that mainly feeds on algae at the bottom of the pond. Even its feed is ground from soybean flour and its body is very sensitive. Therefore, it must be taken care of very carefully. bother

Method used

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  • Application of milkfish fish scale collagen protein
  • Application of milkfish fish scale collagen protein
  • Application of milkfish fish scale collagen protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Raw material: Taiwanese milkfish fish scales

[0051] 1. Wash the fish scales with water;

[0052] 2. Soak in hot water at 90°C for 10 minutes, then filter with a filter cloth, then soak in 95% alcohol for 5 minutes to remove surface protein and impurities and fishy smell, repeat this step 3 times;

[0053] 3. Oven-dried at 50°C, and ground into a powder of less than 100 mesh (100mesh);

[0054]4. Take step 3 fish scale powder and phosphate buffer solution (pH 8), the ratio is 1:100, put it in a sterilizing pot and heat it at 120°C for 30 minutes;

[0055] 5. After cooling, add alkaline protease (Alcalase) with a final concentration of 460U / mL and flavor enzyme (Flavouzyme) with a final concentration of 180U / mL to the product of the above step 4, and then proceed at pH 8.0 and temperature 50°C Shock response (150rpm);

[0056] 6. The reaction time is 3.0 hours. After the reaction is completed, place it in a boiling water bath for 10 minutes to terminate the reaction,...

Embodiment 2

[0061] 1) Antioxidant efficacy - evaluation of scavenging DPPH free radicals (DPPH free radical scavenging)

[0062] Test method: Dissolve the sample in water to make the sample a clear test solution. Take 4ml of samples with different concentrations, add 1ml of freshly prepared 10mM DPPH in DMSO solution, oscillate and mix evenly, and after standing at room temperature for 30min, use a spectrophotometer to detect the absorbance at 517nm.

[0063] Test results: As the concentration of milkfish scale collagen (MSCP) increases, the efficiency of scavenging DPPH free radicals is higher. Milkfish scale collagen (MSCP) scavenging DPPH free radicals for EC 50 1mg / ml.

[0064] see results figure 1 .

[0065] 2) Antioxidant effect--inhibiting ABTS cationic free radicals (ABTS free radical scavenging)

[0066] Test method: This test is based on the configuration concentration of peroxidase 44unit / ml, H20275μM, ABTS 750μM, standard water-soluble VE (trolox) 10mg / ml. Mix peroxidase...

Embodiment 3

[0074] Anti-inflammatory efficacy--inhibition of lipoxygenase test

[0075] Test method: Take 1 μl of sample and add 2 μl of Lox-1 (135 units), then use 1.5 μl of 10 mM linoleic acid as the substrate to cause the reaction, and add 95.5 μl of Tris-HCl buffer (pH 9.0). The control group was based on the same amount of DMSO, after being completely uniformly mixed, the absorbance value was measured at 234nm.

[0076] Test results: Milkfish scale collagen (0.5 and 1 mg / ml) inhibited lipoxygenase (lipoxygenaseisoenzymes Lox-1) activity by 52.3% and 100%, respectively. Milkfish scale collagen (MSCP) inhibits lipoxygenase activity with an EC50 of 475.9 mg / ml.

[0077] see results Figure 4

[0078] Anti-inflammation Efficacy--Scavenging Nitric Oxide Content Test

[0079] Test method: Take 98 μl sodium nitroprusside (sodium nitroprusside, 5mM) and add 2 μl sample to incubate at 25°C for 150 minutes, add 100 μl Greiss Reagent (0.1% naphthylenediaminedihydrochloride naphthalene diami...

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Abstract

The invention discloses application of milkfish fish scale collagen protein which has the effects of oxidation resistance, inflammation resistance, DNA protection capability, moisture retention capability, wrinkle resistance function, skin whitening effect and the like and can be respectively applied to corresponding components in cosmetics.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the use of milkfish low molecular weight fish scale collagen. Background technique [0002] The scientific name of milkfish is Chanoschanos, and its English name is milkfish. It is a vegetarian fish that mainly feeds on algae at the bottom of the pond. Even its feed is ground from soybean flour and its body is very sensitive. Therefore, it must be taken care of very carefully. bother. Milkfish has been cultured in Taiwan for more than 300 years, and it is the product with the most Taiwanese characteristics. For more than 300 years, milkfish has been a delicious and nutritious source of protein, and it is also an important fish species in Taiwan, so it has been given the honorary title of "Taiwan's No. 1 Fish". In Tainan County, there are as many as 5,000 hectares of farming area. At present, there is also farming in the southeast coast of the mainland. [0003] Milkfi...

Claims

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Application Information

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IPC IPC(8): A61K8/65A61Q19/08A61Q19/00A61Q19/02C12P21/06C07K1/34
CPCA61K8/65A61K2800/782A61Q19/00A61Q19/02A61Q19/08C07K14/78C12P21/06
Inventor 吴黉坦王贵弘梁家华陈煜沛
Owner XIAMEN MEDICAL COLLEGE
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