Genome DNA (Deoxyribonucleic Acid) preservation solution of saliva and oral cavity swab

Abstract

The invention discloses a genome DNA (Deoxyribonucleic Acid) preservation solution of a saliva and oral cavity swab. The genome DNA preservation solution is characterized in that the preservation solution comprises the following components according to the concentration of 1 liter of the preservation liquor: 0.10 to 0.2 percent of SDS (sodium dodecyl sulfate), 0.90 percent of NaCl, 5 to 15mM of Tris(trihydroxymethylaminomethane), 20 to 25mM of EDTA (Ethylene Diamine Tetraacetic Acid) and 150 to 160mg of proteinase K. The preservation solution of the saliva and oral cavity swab takes the SDS,Tris-HCl, the EDTA, the NaCl and the proteinase K as main components and can be used for preserving saliva. The NaCl can be used for maintaining osmotic pressure of cells; an SDS surfactant can be used for damaging a protein component and inhibiting the breeding of bacteria under the action of the proteinase K and the degradation of DNA is easy to cause; and the Tris-HCl and the EDTA can be used for protecting free DNA. The DNA preservation solution provided by the invention is a preservation solution aiming at the preservation of the oral cavity swab and can be used for preserving oral cavitycells relatively well; the DNA preservation solution is more sanitary and the bacteria are not easy to breed; the preservation time is 3 to 5 times longer than the preservation time in an envelope; and a preparation method is simple and has relatively good social value.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a saliva and buccal swab genome DNA protection solution. Background technique [0002] Nucleic acid DNA samples extracted from human tissues and body fluids are widely used in forensic identification, disease detection and treatment and other fields. At present, researchers and clinicians mainly use anticoagulant blood to extract DNA. However, agglutination occurs after anticoagulant blood is stored for a long time, which makes the extraction process inconvenient. At the same time, DNA extraction through blood collection still has the following inconveniences: (1) Professional training is required for blood collection personnel; (4) The throughput is low, and it is impossible to collect a large number of samples anytime and anywhere. [0003] In recent years, scientific research and medicine have gradually adopted saliva and oral swab sampling to obtain individual DNA, because ther...

AI Technical Summary

Problems solved by technology

At present, researchers and clinicians mainly use anticoagulated blood to extract DNA. However, the agglutination phenomenon occurs after the anticoagulated blood is stored for a long time, which causes inconvenience to the extraction process.

At the same time, DNA extraction through blood collection still has the following inconveniences: (1) Professional training is required for blood collection personnel; (4) The throughput is low, and it is impossible to collect a large number of samples anytime and anywhere

Images