180 results about "Distinctin" patented technology

The invention describes a process for determining a biological state through the discovery and analysis of hidden or non-obvious, discriminatory biological data patterns. The biological data can be from health data, clinical data, or from a biological sample, (e.g., a biological sample from a human, e.g., serum, blood, saliva, plasma, nipple aspirants, synovial fluids, cerebrospinal fluids, sweat, urine, fecal matter, tears, bronchial lavage, swabbings, needle aspirantas, semen, vaginal fluids, pre-ejaculate.), etc. which is analyzed to determine the biological state of the donor. The biological state can be a pathologic diagnosis, toxicity state, efficacy of a drug, prognosis of a disease, etc. Specifically, the invention concerns processes that discover hidden discriminatory biological data patterns (e.g., patterns of protein expression in a serum sample that classify the biological state of an organ) that describe biological states.

The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab′ fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno-labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.

The invention provides a reagent for diagnosing cervical cancer, which can not only detect the presence or absence of cervical cancer but can also distinguish squamous cell carcinoma and adenocarcinoma from each other, a method for screening cervical cancer by using the reagent, particularly a screening method capable high speed processing by utilizing flow cytometry. The reagent comprises a first labeled antibody reacting with gland cells, a second labeled antibody reacting with adenocarcinoma cells and a third labeled antibody reacting with atypical cervical squamous cells, the antibodies being labeled with mutually distinguishable labels respectively. Preferably, at least one selected from the group consisting of MUC1 antibody, cytokeratin 7 antibody, and cytokeratin 18 antibody is used as the first labeled antibody, at least one selected from the group consisting of cytokeratin 8 antibody and HIK1083 antibody is used as the second labeled antibody, and at least one member selected from the group consisting of NMP179 antibody, p16[INK4A] antibody, Ki-67 antibody, p53 antibody, p21 antibody, EMA antibody, CEA antibody and MIB-1 antibody is used as the third labeled antibody.

The invention relates to an alkaline pectinase genetic engineering bacteria and a construction method thereof. The construction method comprises the steps: separating chromosomal DNA from a strain for producing alkaline pectinase, designing a primer, obtaining a target gene by PCR, constructing a recombinant plasmid, expressing in Bacillus subtilis, measuring gene sequence and carrying out comparative analysis. The gene obtained and expressed in the method is different from the enzyme gene sequences which have been reported and has the differences on the composition of individual amino acids;the culture medium adopted for fermentation of the engineering bacteria is applicable to the industrial production, an expression host is protease deficient which is conducive to the separation, the purification and the stable preservation of enzyme in the late stage, and the enzyme activity of fermentation liquid of the engineering bacteria is improved by 22 times compared with the starting strain, which can achieve 330U / ml.

The invention provides a preserving fluid and a preserving method for maintaining high cell activity of a tissue sample, wherein the preserving fluid comprises the components: ion buffer solution components, carbohydrate components,, components avoiding the generation of ice crystals in and out solutions of tissue cells at a low temperature of 0-6 DEG C, components providing supplement, antioxidant and anti-apoptosis components for anabolism of the tissue cells; the preserving fluid has good biocompatibility, does not contain toxic or harmful components, and also does not contain components such as animal-derived protein, antibiotics and hormones influencing tissue gene expression. Different from traditional ultralow-temperature cryopreservation (-80 DEG C), the cryopreservation method disclosed by the invention can effectively realize low-temperature storage (0-6 DEG C) of fresh tissue samples of human beings and animals, maintains high cell activity of tissues, maintains tissue morphology, and is used for preservation and transportation of the tissue samples. Meanwhile, the nucleic acid integrity of the tissue and the stability of gene expression can be effectively maintained, the original state of the tissue is maintained, and the method can be used for gene detection, scientific research and other related experiments.

The invention discloses a PCR primer pair used for identifying the genetic sex of Pseudobagrus ussuriensis, and a rapid identification method thereof. The identification method comprises the steps of examined individual tissue drawing, genome DNA extraction, sex specific DNA fragment PCR amplification, agarose gel electrophoresis, ultraviolet light detection shooting and examined individual genetic sex determination. High-quality genome DNA is extracted through an optimized process, a sex specific DNA fragment is amplified, and the existence or not and the existence characteristic of the sex specific fragment are detected under ultraviolet lights, so the genetic constitution of Pseudobagrus ussuriensis is identified, and the genetic sex is discriminated in 1-2d. The PCR primer pair and the rapid identification method provide a rapid identification method for identifying sex reversal fish and super-male fish, provide technical support for breeding all-male fish, and have the advantages of breeding time shortening, great increase of the output, breeding cost reduction, and profound economic and social values.

The invention discloses a PCR-RFLP primer and method for rapidly identifying the genotypes of duck circoviruses. The primer comprises an upstream primer body P1:5'-TGCGCCAAAGAGTCGACATAC-3' and a downstream primer body P2:5'-CAAATTGGTCTCAGTAGTTTATT-3'. According to the method, distinguishing is carried out through the difference of restriction enzyme cutting sites contained in coding regions of copied protein genes of the duck circoviruses in different genotypes (the first genotype and the second genotype). The method includes the steps that DNA is extracted, Kpn I restriction enzyme cutting is carried out after PCR amplification is carried out to obtain corresponding target fragments, and then RFLP analysis is carried out. The identification method is rapid and easy to operate and high in accuracy.

The invention discloses a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method for distinguishing a goose parvovirus from a muscovy duck parvovirus. According to the PCR-RFLP method, NS gene sequence digestion site difference is used for distinguishing the goose parvovirus from the muscovy duck parvovirus. The method comprises the following steps of: extracting DNA (deoxyribonucleic acid), carrying out PCR amplification to obtain an NS gene segment, and carrying out RFLP analysis after EcoRI digestion is carried out. The PCR-RFLP method for distinguishing the goose parvovirus from the muscovy duck parvovirus is simple and high in efficiency and accuracy.

The invention discloses a PCR-RFLP primer for distinguishing DHV-1 and a new serotype and a method for distinguishing the DHV-1 and the new serotype. The primer is an upstream primer P1:5'-CAATTGAGGACATGGCTAAGAAA-3' and a downstream primer P2:5'-TCAGAGASCCRTCRAAWCCAGAAA-3. According to the method, distinguishing is conducted according to the DHV-1 and the restriction enzyme cutting site difference contained in a new serotype coding area and includes the steps that RNA extraction is conducted, and after corresponding target fragments are obtained through RT-PCR amplification, RFLP analysis is conducted after XhoI restriction enzyme cutting. The distinguishing method is simple and fast in operation and high in accuracy.

An angiotensin IIreceptorI-type immune peptide section and its use are disclosed. The peptide section is selected from the amino-acid sequence as following: SEQ ID NO:181-187, amino-acid sequence of peptide section is Ala-Phe-His-Tyr-Glu-Ser-Arg; its analog is Ala-Phe-His-Tyr-Glu-Ser-Gln. It has excellent treatment performance, antigenicity and / or immunodominance. The use is carried out by feeding medicine by injecting for 0-8weeks. It can be used to treat primary hypertension as vaccine or medicinal composition.

The invention relates to a kit for simultaneously extracting DNA and RNA and a using method thereof. The kit comprises the following reagents: a cell lysis solution, a nucleic acid binding solution, anucleic acid adsorption material, an eluate A, a deproteinized rinsing solution, a demineralized rinsing solution and an eluate B. According to the method, the DNA is selectively eluted, and the RNAis continuously retained on the adsorption material, so that the effect of fractionating the DNA from the RNA is achieved. Different from a TRIzol method for simultaneously extracting DNA and RNA, themethod provided by the invention uses few reagents, does not use phenol, chloroform and other toxic solvents, can be used on a nucleic acid purification instrument, and has the advantages of rapidness, high efficiency, high flux, environmental protection and capability of automatically and simultaneously extracting DNA and RNA.

A method and kit for synthesizing a nucleic acid molecule comprising at least one non-canonical nucleoside triphosphate using a double-mutant polymerase having a reduced discrimination between canonical and non-canonical substrates is disclosed. The method comprises incubating a template nucleic acid in a reaction mixture comprising the mutant nucleic acid polymerase and the appropriate canonical and non-canonical nucleoside triphosphates which are desired substrates for the mutant nucleic acid polymerase. The present invention is also a method of determining the sequence of a nucleic acid molecule using the mutant polymerase to create a nucleic acid molecule comprising at least one non-canonical nucleoside triphosphate.

The invention discloses a white rot Inonotus hispidus mutant strain T906 for laccase production and a preparation method thereof. The mutant strain T906 (CoriolopsisgallicaT906) mentioned in the invention is preserved in China Center for Type Culture Collection. The preservation number is CCTCC NO: M2011317, the preservation date is September 15, 2011 and it is preserved in Wuhan University, Wuhan, China. Wild Inonotus hispidus (preservation number being cfcc88387) purchased from China Forestry Culture Collection Center is used as an original strain, and the mutant strain is obtained by ultraviolet and nitrosoguanidine combined mutation screening. Under an optimum condition, the laccase production of the mutant strain reaches 213U / mL. The mutant strain has mycelial morphology and cultural characteristics which are obviously different from the original strain, does not degenerate after serial passages for over 30 times and has genetic stability.

The present invention provides labeling reagents and methods for labeling primary antibodies and for detecting a target in a sample using an immuno-labeled complex that comprises a target-binding antibody and one or more labeling reagents. The labeling reagents comprise monovalent antibody fragments or non-antibody monomeric proteins whereby the labeling proteins have affinity for a specific region of the target-binding antibody and are covalently attached to a label. Typically, the labeling reagent is an anti-Fc Fab or Fab′ fragment that was generated by immunizing a goat or rabbit with the Fc fragment of an antibody. The present invention provides for discrete subsets of labeling reagent and immuno-labeled complexes that facilitate the simultaneous detection of multiple targets in a sample wherein the immuno-labeled complexes are distinguished by i) a ratio of label to labeling reagent, or ii) a physical property of said label, or iii) a ratio of labeling reagent to said target-binding antibody, or iv) by said target-binding antibody. This is particularly useful for fluorophore labels that can be attached to labeling reagents and subsequently immuno-labeled complexes in ratios for the detection of multiple targets.

The invention discloses a wild ginseng and cultivated ginseng multiple PCR test kit, which is characterized by containing: buffer solution, 12.5mM of deoxy-ribonucleoside triphosphate (dNTP), 0.1mM of one of a primer 1 and a primer 2, Taq DNA polymerase, a sample DNA to be tested and a double-distilled water identification reaction system; or the buffer solution, 12.5 mM of dNTP, 0.1 mM of one of the primer 1 and the primer 2, Taq DNA polymerase, wild ginseng and cultivated ginseng DNA 1:1 mixture and a double-distilled water positive reference reaction system; or the buffer solution, 12.5 mM of dNTP, 0.1 mM of one of the primer 1 and the primer 2, Taq DNA polymerase, araliaceae congeneric DNA 1:1 mixture, and a double-distilled water negative reference reaction system. The detection and identification method comprises the steps of designing two pairs of specific oligonucleotide primers of wild ginseng and cultivated ginseng mitochondrion DNAs, designing two pairs of specific oligonucleotide primers of synthetic wild ginseng and cultivated ginseng mitochondrion DNAs, determining a reaction process, determining result and the like. The method can accurately determine the specificity of both the wild ginseng and the cultivated ginseng, and the detection result is reliable.

The invention provides a dual fluorescence quantification RT-PCR (reverse transcription-polymerase chain reaction) detection kit, comprising a deoxynucleoside triphosphate mixture, MgCl2, an RNA enzyme inhibitor, a Moloney murine leukemia virus reverse transcriptase, a DNA polymerase, a influenza virus standard and a reference substance. Based on sequence analysis of present pervasive A H1N1 influenza virus, the invention provides a multiple fluorescence quantification PCR molecular biology gene diagnosis method and a diagnostic kit which are rapid, specific, accurate and sensitive. In addition, one reaction tube can simultaneously detect and differentiate an influenza A virus or an influenza B virus in 2h, so as to improve influenza detection.

The invention discloses alkaline thermal-stability SGNH family esterase EstD1. The amino acid sequence of the alkaline thermal-stability SGNH family esterase EstD1 is one of the amino acid sequence (1) shown in SEQ ID NO.1 and the amino acid sequence (2), wherein one or more pieces of amino acid are added or replaced or lacked or inserted, shown in SEQ ID NO.1. The invention further discloses a gene EstD1 of the alkaline thermal-stability SGNH family esterase EstD1, the nucleotide sequence of the gene EstD1 is one of the nucleotide sequence (1) shown in SEQ ID NO.2 and the nucleotide sequence (2), wherein one or more pieces of nucleotide are added, replaced, lacked or inserted, shown in SEQ ID NO.2, the nucleotide sequence (3) formed through hybridization with the nucleotide sequence (1) or nucleotide sequence (2) under strict conditions and the nucleotide sequence (4) which is different from the nucleotide sequence (1), the nucleotide sequence (2) and the nucleotide sequence (3) due to the degeneracy of a genetic codon. The alkaline thermal-stability SGNH family esterase EstD1 can be used for deacetylation of 7-ACA.

The invention discloses novel radioactive 18F labeled aromatic amino acids, which is characterized in that one end of the acids is provided with F substituted alkoxy benzoyl structure, and the other end of the derivatives is provided with alpha-amino acid structure; substituent R1 is positioned on an alpha site of carboxyl group and is phenyl group, benzyl group or 3-indole methyl group; R2 is methoxyl group; and n is a number between 1 and 5. The substituent R1 is positioned on an alpha site of carboxyl group and is phenyl group, benzyl group or 3-indole methyl group; the R2 is methoxyl group; and the n is a number between 1 and 5. Compounds improve fat solubility. Different amino acid structures are introduced into the structure, and F in the structure is 19F and 18F. The substituent R1 is positioned on an alpha site of carboxyl group and is phenyl group, benzyl group or 3-indole methyl group; the R2 is methoxyl group; and the n is a number between 1 and 5. The substituent R1 is positioned on an alpha site of carboxyl group and is phenyl group, benzyl group or 3-indole methyl group; the R2 is methoxyl group; and the n is a number between 1 and 5. Compared with the prior art, the 18F labeled amino acid derivatives provided by the invention have better discrimination degree of biological distribution, the potential of being used as a tumor imaging agent (particularly a brain tumor imaging agent), as well as the characteristics of simple preparation and high labeling rate.

The invention discloses a method for preparing a molecular bar code system for describing and discriminating animals and plants, which comprises the following steps: (1) acquiring monomorphic or polymorphic stripe data of animal and plant materials obtained by single or various molecular mark technologies and forming a mark set as a molecular mark set for discriminating a species, a subspecies, a variant, a breed and an inbred line or a variant single strain; (2) converting and storing a primer, a monomorphic or polymorphic mark and an analyzing result used in the name and the molecular mark of the molecular mark set obtained by the animal and plant materials by a two-dimensional bar code technology and expressing in a bar code way. The invention also discloses an application of the molecular bar code system obtained by the preparation method. The molecular bar code system is used for authenticating, discriminating, identifying or authorizing the species, the subspecies, the variant, the breed and the inbred line or variant single strain or storing and updating the data in ways of a computer, a network, and the like.

The invention relates to a haemophilus parasuis detection kit and a detection method thereof, and belongs to the technical field of molecular biology. The detection kit comprises a primer pair, PCR Mix, a position control and dd H2O. The haemophilus parasuis detection kit disclosed by the invention has the primer pair designed according to an mviN gene sequence in a high conserved domain, is good in specificity, and can accurately distinguish the haemophilus parasuis strain LC from haemophilus paragallinarum, actinobacillus pleuropneumoniae, pasteurella muhocida, arcanobacterium pyogenes, staphylococcus aureus and streptococcus suis; the detection kit and the detection method provided by the invention are high in sensitivity, short in consumed time, accurate in detection, and important in significance of monitoring haemophilus parasuis reproduction, disease occurrence and prevalence as well as timely control of the disease.

The invention relates to an anti-African swine fever virus p54 protein monoclonal antibody and a preparation method and application thereof. According to the invention, polypeptide capable of simulating conservative p54 protein NTD is designed and synthesized, and the polypeptide is coupled with BSA to serve as immunogen, an anti-ASFVp54 protein monoclonal antibody is prepared by immunizing a BALB / c mouse through an immunological method, the obtained antibody can specifically identify and bind to an N-terminal region of p54 protein, and the recognition region (aa 1-29) is different from the existing commercial monoclonal antibody. The invention provides gene sequences of a heavy chain variable region and a light chain variable region of the antibody. On this basis, the monoclonal antibodyof the invention can be obtained by adopting a conventional genetic engineering method or a protein engineering method. The monoclonal antibody is high in specificity and high in sensitivity, capableof specifically reacting with the ASFV HLJ / 18 virus strain, without reaction with CSFV, PCV2, PRRSV, PEDV, PRV and other swine viruses, thus laying a foundation for the research of ASFV etiology and pathogenesis and the clinical detection research of ASFV pathogens.

The invention discloses a PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) method for differentiating duck circovirus from goose circovirus. The PCR-RFLP method is used for differentiating by utilizing Rep protein gene sequence enzyme cutting site difference and comprises the following steps of: extracting DNA (Deoxyribonucleic Acid), carrying out PCR amplification to obtain a Rep protein gene segment, and carrying out RFLP analysis after carrying out XhoI digestion. The PCR-RFLP method disclosed by the invention is simple and higher in efficiency and accuracy.

The invention discloses an associated protein for regulating and controlling round-leaf characteristics of cucumbers as well as a coding gene thereof and application thereof. An amino acid sequence ofthe protein is as shown in SEQ ID NO:2; protein kinase, which belongs to an AGC kinase family, of serine / threonine is coded by a PID gene; a coding gene of the protein is a base sequence as shown ina sequence table SEQ ID NO:1; guanine (G), at a site 1092bp, of the base sequence is substituted by adenine (A), Val (valine) at a site 346 of a coded amino acid residue sequence is mutated to be Met(methionine); the protein inhibits polar translocation of auxin, causes polar defect of auxin transport, has a blade edge smooth plant phenotype, has unapparent saw-tooth bugles, and has nearly roundleaves; a main root is not developed, and a main lateral root does not have obvious difference; male and female flowers are free of calyxes, and female flowers are not developed and the like. The geneand the protein can provide a basis for improving cucumber leaf type, increasing the yield, cultivating ideal plants and researching an auxin signal channel molecule regulation mechanism.

The present invention provides methods for determining a nucleic acid sequence by performing successive cycles of duplex extension along a single stranded template. The cycles comprise steps of extension, ligation, and, preferably, cleavage. In certain embodiments the methods make use of extension probes containing phosphorothiolate linkages and employ agents appropriate to cleave such linkages. In certain embodiments the methods make use of extension probes containing an abasic residue or a damaged base and employ agents appropriate to cleave linkages between a nucleoside and an abasic residue and / or agents appropriate to remove a damaged base from a nucleic acid. The invention provides methods of determining information about a sequence using at least two distinguishably labeled probe families. In certain embodiments the methods acquire less than 2 bits of information from each of a plurality of nucleotides in the template in each cycle. In certain embodiments the sequencing reactions are performed on templates attached to microparticles, which are immobilized in or on a semi-solid support or attached to a substrate. The invention further provides sets of labeled extension probes containing phosphorothiolate linkages or trigger residues that are suitable for use in the method. In addition, the invention includes performing multiple sequencing reactions on a single template by removing initializing oligonucleotides and extended strands and performing subsequent reactions using different initializing oligonucleotides. The invention further provides efficient methods for preparing templates, particularly for performing sequencing multiple different templates in parallel. The invention also provides methods for performing ligation and cleavage.

The invention relates to a nucleic acid detection test strip for distinguishing a Brucella melitensis vaccine strain S19 from a natural infection strain, relates to the field of Brucellosis detection,and solves the problems that a conventional pathogeny detection method cannot detect the Brucella melitensis vaccine strain S19 and the natural infection strain at the same time. The detection test strip comprises a Brucella melitensis forward primer of which the sequence is shown as SEQID NO:1, a Brucella melitensis reverse primer of which the sequence is shown as SEQID NO:2, and a Brucella melitensis probe of which the sequence is shown as SEQID NO:3. The sequence of the forward primer of the Brucella melitensis vaccine strain S19 is shown as SEQID NO:4, the sequence of the reverse primer of the Brucella melitensis vaccine strain S19 is shown as SEQID NO:5, and the sequence of the probe of the Brucella melitensis vaccine strain is shown as SEQID NO:6. The test strip can specially detectthe disferences between Brucella melitensis natural infection and artificial immunity, and has high specificity and sensibility.

The invention relates to the fields of medical treatment evaluation and detection technology, and in particular relates to a method for preparing an intestinal mucosa injury animal model and applications of the prepared intestinal mucosa injury animal model. According to the scheme, the medicine L-MSO having good water solubility and realizing convenient and simple gavaging operation is adopted, the mouse intestinal mucosa injury is conveniently and stably induced, and meanwhile, the corresponding tissue pathological change and inflammatory factor change are caused. For the preparation method provided by the invention, the intestinal mucosa injury animal model is prepared from the metabolism injury direction obviously different from the molding direction of immune injury inducing in the prior art, particularly, the low-concentration L-MSO is adopted for preparing the common injured model, wherein the intestinal mucosa of the laboratory mouse has the mild-moderate injury, the high-concentration L-MSO is adopted for preparing the seriously injured model, wherein the intestinal mucosa of the laboratory mouse has the severe injury; the prepared intestinal mucosa injury model has the significant values for the research and practice of inflammatory bowel diseases.

The invention belongs to the technical field of biomedicine, and specifically relates to an intestinal microbial marker for distinguishing autoimmune hepatitis patients from healthy people and applications of the intestinal microbial marker. The intestinal microbial marker for distinguishing the autoimmune hepatitis patients from the healthy people provided by the invention is composed of microorganisms indicated by at least one gene shown in SEQ ID NO:1-5, and the microbial genes are obviously enriched or reduced in human intestines. The invention also provides an application of a detection reagent to preparation of a detection kit for distinguishing the autoimmune hepatitis patients from the healthy people, and an application of the detection reagent to establishing of an intestinal microbial model for distinguishing the autoimmune hepatitis patients from the healthy people. Therefore, the microbial gene distinguishing model provided by the invention realizes good distinguishing ability in the autoimmune hepatitis patients and the healthy people.

The invention relates to a Chinese date honey characteristic protein and isolation and purification thereof; firstly, eight single-flower honey is subjected to SDS-PAGE, a result finds out that the Chinese date honey has one protein spectral band at about 17 ku while protein electrophoresis spectrograms of other honeys do not contain the protein spectral band at about 17 ku; in addition, the molecular weight of the protein has quite large difference from the molecular weights of other protein spectral bands, so as to facilitate separation. Therefore, the protein can be used as the characteristic protein of the Chinese date honey different from other honeys. For making the molecular weight and structure information of the protein clear, an LC-Chip / ESI-QTOF-MS method is used for identification. The operation is simple, the separation effect is good, the feasibility is high, and the new method is provided for true and false identification of the Chinese date honey.

The invention discloses a triple fluorescent quantitative PCR primer, probe and kit for identifying pseudorabies virus classical strains, variant strains and Bartha-K61 vaccine strains. The sequences of the primer are represented by SEQ ID No. 8-9 and SEQ ID No. 14-15, and the sequences of the probe are represented by SEQ ID No. 1, 4 and 6. According to the kit, the pseudorabies virus classical strains, the variant strains and the Bartha-K61 vaccine strains can be accurately identified based on TaqMan probes marked with different fluorescence signals, and the kit has good sensitivity and specificity and can be applied to the discriminative detection of pseudorabies virus strains.

The invention relates to preparation of an anti-PML protein nuclear location signal antibody and application thereof in APL diagnosis. The antibody is prepared from an amino acid sequence shown in SEQ ID NO:1, namely NLS polypeptide as immunogen. The antibody can be used for distinguishing the PML protein from the PML protein lack of a nuclear localization signal, and can be applied to preparation of a diagnostic agent for acute promyelocytic leukemia. In addition, the invention also provides a location method of the PML protein. Therefore, the foundation is established for further research of the PML for inducing cell apoptosis on the gene transcription level to inhibit tumor growth. Meanwhile, wild PML protein and PML (NLS-) protein are differentially detected, and a novel basis is provided for clinical diagnosis of APL and intensive study of pathogenesis of the APL.