Method for preparing molecular bar code system for describing and discriminating animals and plants and application thereof

A molecular bar code, animal and plant technology, applied in the field of molecular bar code system preparation, can solve the problem of single molecular marker types, no set of standards for marker types and numbers, no new species, close relatives or recently differentiated varieties classification or screening. Issues such as direct availability and sharing

Inactive Publication Date: 2010-04-14
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Secondly, due to the reasons of seed production technology and operating profit, there are many fake and inferior varieties on the market, including low-purity hybrids and unsalable varieties that are shoddy and pretend to be good varieties.
Third, it is not uncommon to use high-quality hybrids with high market share for separation and purification, and illegal operation and theft of varieties are also common.
Although molecular markers are also used in the study of biological evolution, phylogeny, and animal and plant classification, the molecular markers currently used are of a single type and the number is not large, so they can only be limited to the classification or varieties

Method used

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  • Method for preparing molecular bar code system for describing and discriminating animals and plants and application thereof
  • Method for preparing molecular bar code system for describing and discriminating animals and plants and application thereof
  • Method for preparing molecular bar code system for describing and discriminating animals and plants and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1, Screening of Cruciferous Crops and Construction of Molecular Barcodes

[0043] 74 cultivar materials (Table 1) of Brassica rapa, cabbage (B.oleracea), mustard (B.juncea) and radish (Raphanus sativus) crops were selected for molecular barcode construction and variety screening.

[0044] Table 1. Information on 74 varieties of cruciferous crops

[0045] Species / varieties (Latin name)

Variety

Species / varieties (Latin name)

Variety

Brassica rapa ssp. chinensis var.

pekinensis

yellow sprouts

Brassica rapa ssp.

rapifera

Yuhuan Dish

Brassica rapa ssp. chinensis var.

pekinensis

Yellow Bud 14

Brassica rapa ssp.

rapifera

Nanxi dish

Brassica rapa ssp. chinensis var.

pekinensis

Zhebai No.4

Brassica rapa ssp.

rapifera

Wenkang No.1

Brassica rapa ssp. chinensis var.

pekinensis

Zhebai No. 6

Brassica rapa ssp.

rapifera

...

Embodiment 2

[0067] Example 2, the screening of the genus Suis and the construction of molecular barcodes

[0068] Seventeen Chinese local pig breeds and one wild boar breed were selected to construct molecular barcodes (Table 2).

[0069] Table 2. Information on 17 Chinese local pig breeds and 1 wild boar breed

[0070]

[0071] For each variety material in Table 2, the following steps are carried out respectively:

[0072] (1) Acquisition of Genomic DNA Sequence

[0073] 5 mL of blood was collected from the anterior vena cava of pigs, and frozen with EDTA anticoagulant. Genomic DNA was extracted by traditional phenol / chloroform extraction method, and the purity of the product was about 80% to 97%. After TE was dissolved, it was diluted to a final concentration of 50ng / μL.

[0074] (2) Using fluorescent AFLP molecular markers to obtain genome information of each material

[0075] Enzyme digestion of genomic DNA: Take 250ng of genomic DNA and perform double digestion with EcoR I and...

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Abstract

The invention discloses a method for preparing a molecular bar code system for describing and discriminating animals and plants, which comprises the following steps: (1) acquiring monomorphic or polymorphic stripe data of animal and plant materials obtained by single or various molecular mark technologies and forming a mark set as a molecular mark set for discriminating a species, a subspecies, a variant, a breed and an inbred line or a variant single strain; (2) converting and storing a primer, a monomorphic or polymorphic mark and an analyzing result used in the name and the molecular mark of the molecular mark set obtained by the animal and plant materials by a two-dimensional bar code technology and expressing in a bar code way. The invention also discloses an application of the molecular bar code system obtained by the preparation method. The molecular bar code system is used for authenticating, discriminating, identifying or authorizing the species, the subspecies, the variant, the breed and the inbred line or variant single strain or storing and updating the data in ways of a computer, a network, and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a preparation method of a molecular barcode system for describing and identifying animals and plants (species, subspecies, varieties, varieties, inbred lines or mutant individual plants), and an application of the molecular barcode system. Background technique [0002] Effective identification and screening of crop varieties has never been as important as it is today. In addition to the fact that there are many kinds of traditional crops and the variety resources are very rich, it is also caused by the following factors. First, with the development of agricultural production, the genetic basis of many species has become narrower and narrower while breeding has become more and more effective. Secondly, due to the reasons of seed production technology and operating profit, there are many fake and inferior varieties in the market, including low-purity hybrids and unsalable ...

Claims

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Application Information

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IPC IPC(8): G06K19/06G06F17/30C12Q1/68
Inventor 曹家树黄鹂赵晓枫朱熠鹏
Owner ZHEJIANG UNIV
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