Wild ginseng and cultivated ginseng multiple polymerase chain reaction (PCR) test kit and identification method

Abstract

The invention discloses a wild ginseng and cultivated ginseng multiple PCR test kit, which is characterized by containing: buffer solution, 12.5mM of deoxy-ribonucleoside triphosphate (dNTP), 0.1mM of one of a primer 1 and a primer 2, Taq DNA polymerase, a sample DNA to be tested and a double-distilled water identification reaction system; or the buffer solution, 12.5 mM of dNTP, 0.1 mM of one of the primer 1 and the primer 2, Taq DNA polymerase, wild ginseng and cultivated ginseng DNA 1:1 mixture and a double-distilled water positive reference reaction system; or the buffer solution, 12.5 mM of dNTP, 0.1 mM of one of the primer 1 and the primer 2, Taq DNA polymerase, araliaceae congeneric DNA 1:1 mixture, and a double-distilled water negative reference reaction system. The detection and identification method comprises the steps of designing two pairs of specific oligonucleotide primers of wild ginseng and cultivated ginseng mitochondrion DNAs, designing two pairs of specific oligonucleotide primers of synthetic wild ginseng and cultivated ginseng mitochondrion DNAs, determining a reaction process, determining result and the like. The method can accurately determine the specificity of both the wild ginseng and the cultivated ginseng, and the detection result is reliable.

Description

technical field [0001] The invention relates to the technical field of detection of traditional Chinese medicines, in particular to a DNA detection kit and identification method of wild ginseng. Background technique [0002] Ginseng (panax ginseng C.A.Meyer) has many functions such as nourishing and strengthening, nourishing qi and nourishing blood, etc., and is known as "the king of all medicines". It has a history of application and cultivation in my country for more than 2,000 years. One is divided into three types: wild ginseng, wild ginseng and cultivated ginseng. Wild ginseng is a well-known precious medicinal material at home and abroad. It has outstanding curative effect but is expensive. It is mainly distributed in Northeast China, North Korea and Russia. At present, the main resources of wild ginseng in my country are further shrinking, and it has been listed as a national rare and endangered plant. Due to the sharp reduction of forest area and over-excavation, t...

AI Technical Summary

Problems solved by technology

However, these detection techniques are difficult to fundamentally represent the genetic markers of ginseng medicinal variation

However, due to the long life cycle and frequent cross-pollination of ginseng, its population is a hybrid, and the individual is a heterozygote, and due to the biological characteristics of ginseng itself

This situation severely restricts the further development of ginseng quality research, so no specific DNA molecular markers that can stably and effectively distinguish wild ginseng from cultivated ginseng have been obtained so far.

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