A PCR-based rapid genetic sex identification method for
Scylla Paramamosain is disclosed. The method includes (1) designing control primers, and designing female-
specific primers by utilizing a base mismatch process; (2) extracting
genome DNA of a
Scylla Paramamosain individual to be tested; (3) by using the control primers and female-
specific primers respectively, and by using the
genome DNA of
Scylla Paramamosain as a template, performing PCR amplification at an annealing temperature of 65 DEG C to obtain corresponding PCR products; and (4) detecting the PCR products through electrophoresiswith
agarose gel having a concentration of 1.5% to determine whether the individual is male or female. According to the method, the base mismatch process of the sex-specific SNP site is utilized to design the female-
specific primers, amplification is performed through an optimized PCR
reaction system and a PCR reaction program, and then
electrophoresis with
agarose gel is performed for detection to achieve genetic sex identification of the Scylla Paramamosain. The method has advantages of simple operation, a high accuracy rate, a low cost, good
repeatability and high practicability, and can beused for large-scale
rapid identification for Scylla Paramamosain in the early period of growth and development.