58 results about "Spermatocyte" patented technology

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

The present invention provides a method of cryopreserving sperm that have been selected for a specific characteristic. In a preferred embodiment, the method is employed to freeze sex-selected sperm. Although the cryopreservation method of the invention can be used to freeze sperm selected by any number of selection methods, selection using flow cytometry is preferred. The present invention also provides a frozen sperm sample that has been selected for a particular characteristic, such as sex-type. In preferred embodiments, the frozen sperm sample includes mammalian sperm, such as, for example, human, bovine, equine, porcine, ovine, elk, or bison sperm. The frozen selected sperm sample can be used in a variety of applications. In particular, the sample can be thawed and used for fertilization. Accordingly, the invention also includes a method of using the frozen selected sperm sample for artificial insemination or in vitro fertilization.

The present invention provides methods for preparing cells with highly condensed chromosomes, such as sperm, and methods for detecting and quantifying specific cellular target molecules in intact cells. Specifically, methods are provided for detecting chromosomes and chromosomal abnormalities, including aneuploidy, in intact cells using fluorescence in situ hybridization of cells in suspension, such as sperm cells.

Methods and apparatus are disclosed for processing sperm cells to accomplish preservation for future use while minimizing the adverse effects of such preservation. Sperm cells may be collected from a male animal and subjected to a first preservation step, including potentially a first cryopreservation step. Preserved sperm may then be revived, including potentially by thawing, and treated by any of various processing steps to mitigate the adverse effects of preservation. Treated sperm may then be subjected to a second preservation step, including potentially a second cryopreservation step, perhaps enabling a delayed use of the sperm at a future time.

The invention discloses novel growth hormone releasing hormone analog peptides and an application thereof in preparing medicines for treating infertility. Experiments discover that 2D, 2E or 2F peptide has obviously high hypophysis GH releasing activity and hypophysis hormone releasing specificity. Tested by the conjugation reaction of an in-vitro GHRH dimer peptide and a hypophysis GHRH receptor, 2D, 2E and 2F dimer peptides have extremely high hypophysis receptor binding activity, wherein the 2F dimer peptide has the maximum binding activity. With the 2F peptide as the representative, infertility model treatment finds out that, compared with a normal saline group and a pure cyclophosphamide control group, in the 2F dimer peptide group, the spermatocytes and the spermatogonia in the seminiferous tubules are obviously increased, the seminiferous tubule cells are arranged in order and large in volume, the cavities of the seminiferous tubules are reduced and even disappear, and dose dependency is shown. All the facts indicate that the GHRH peptides with the 2F peptide as the representative have an obvious effect of stimulating the proliferation and the maturation of spermatogonia/oogonia, thereby promoting reproduction; and as a result, the novel growth hormone releasing hormone analog peptides can be applied to medicines for treating infertility.

The invention relates to a three-dimensional inducing method for inducing a spermatogonia stem cell to differentiate into a functional sperm cell in vitro, and belongs to the field of bioengineering. On the basis of acquiring the spermatogonia stem cell, a three-dimensional culture system is established through Matrigel, a key factor for inducing differentiation is added, the spermatogonia stem cell is induced to differentiate in vitro to obtain the sperm cell having a fertilization ability, the sperm cell obtained through induction is identified through an immunocytochemical method, the chromosome number of the sperm cell obtained through induction is detected through fluorescence in situ hybridization (FISH), and the fertilization ability and the viability of the sperm cell obtained through induction are detected through round spermatid injection (ROSI). The application provides an excellent model for the molecular mechanism of the human sperm, and in addition, a functional gamete is provided for an azoospermia patient, so that a male infertility patient can have a child of his own.

Herein are disclosed methods for producing female sperm by incorporating a female's chromosomes into a sperm cell via mostly natural spermatogenesis processes. The methods include transplanting (altered) female stem cells or (altered) cloned germ cells into sterilized testes of a male. Diploid female stem/germ cells are altered in two ways—transdifferentiation to facilitate the expression of spermatogenesis factors (for example, using artificial chromosomes) and/or retrodifferentiation to increase pluripotency and imprinting erasures. The altered cells are transplanted into (artificial) male testes to develop into sperm, which are used to fertilize an egg of a second female, or an egg of the original female. Also disclosed herein are methods for producing male eggs by adding an extra X chromosome to an adult male's germ cells, and cultivating the germ cells in vitro.

The invention relates to the field of cell engineering, and concretely provides a method for in vitro culture of testis tissues and induction to generate spermatid. The method comprises the following steps: 1, preparing culture gel pieces: placing 1.2% agarose gel pieces in a 6-orifice plate, adding a medium, immersing overnight, extracting the used medium, adding the fresh medium, and using the agarose gel pieces as culture gel pieces, wherein the medium comprises 90% of MEM alpha and 10% of KSR; 2, taking 5.5-days and 10.5-12.5-days mouse testes, removing external layer albuginea, and cutting to form block tissue samples; 3, placing the tissues samples on the culture gel pieces, putting the 6-orifice plate in a cell culture box, and culturing; and 4, culturing the 5.5-days mouse testis tissues for 22-25d to obtain round sperms, and culturing the 10.5-12.5-days mouse testis tissues for about 27d to obtain elongated sperms.

The invention relates to a preparation method of a shrimp germ cell chromosome. The preparation method comprises the following steps: culturing testis/ovary by colchicine; performing KCl hypotonic treatment and pre-fixing by a Carnoy fixing solution; fixing by an after-fixing solution, wherein the after-fixing solution comprises absolute ethyl alcohol and glacial acetic acid according to the volume ratio of 1:1; dispersing spermatocytes/oocytes to prepare a cell suspension; dyeing by a Giemsa phosphate buffered solution; and performing microscopical examination under a Motic microscope. The preparation method disclosed by the invention has the following advantages that the operation is simple and efficient and chromosome slides can be mass-produced; sufficient chromosome samples can be fast, simply and conveniently obtained, the obtained samples are uniform in dispersion, shallow in background and easy to observe, and the obtained chromosome number is high in accuracy; the simple and easy-to-operate method is provided for preparing shrimp chromosomes; and the non-toxic fixing solutions are adopted, and the harm to a slide making operator can be reduced.

A method and vector system for delivering of a gene into a human stem cell for therapeutic uses is disclosed. The method includes linking a therapeutic gene to human sperm cells through a linker and fertilizing a human oocyte. The resulting zygote may then be cultured and established as human embryonic stem cells, which may later be differentiated into different types of cells for transplantation into the human body.

The present invention relates to an immunological method that selects spermatozoa having X-chromosome or Y-chromosome. The method of the invention is based on the use of monoclonal antibodies directed against the genus-specific proteins located in the cytoplasm membrane of spermatozoa associated to the action of the classical complement pathway to increase the percentage of one of the gender in the offspring of mammals.

The invention provides a dissociation and separation method for shellfish spermary spermatogenic cells, and belongs to the technical field of the separation of ocean shellfish cells. The dissociationand separation method comprises the following steps of: the spermary of a shellfish during a proliferating phase is broken and is digested by a collagenase IV solution of which the mass concentrationis 0.1% and a trypsin solution of which the mass concentration is 0.25% in sequence, and spermary dissociation is carried out to obtain a single-cell suspension; and Percoll solutions of which the volume concentration is independently 10%, 22% and 35% is prepared, and the Percoll solutions are loaded in the same centrifuge tube in a descending order, the single-cell suspension of the shellfish spermatogenic cells is added into the top layer of the centrifuge tube, and centrifugation is carried out to independently collect three layers of cells. By use of a two-step enzymolysis method, spermatogenic cells in spermary tissues can be successfully dissociated into the single-cell suspension, a cell survival rate can be as high as 96.62%, and the cells can be normally subjected to in vitro normal culture. Meanwhile, high-purity spermatogonia and spermatocytes can be separated, an important material is provided for researching a shellfish gamete generating and breeding mechanism, and the dissociation and separation method has a high guidance meaning and a good application value.

A method is provided herein, wherein the method of capturing a sperm deoxyribo nucleic acid (DNA) in a sample, comprises contacting a lysis solution to the sample comprising at least a sperm cell or a sperm cell lysate to lyse the sperm cell. The sperm cell or sperm cell lysate comprises a protamine-DNA complex. The method further comprises applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex. The antibody binding is followed by capturing the antibody-protamine-DNA complex; and isolating and detecting the sperm DNA from the captured antibody-protamine-DNA complex.