34 results about "Sperm sorting" patented technology

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

This disclosure relates to a system for sorting sperm cells in a microfluidic chip. In particular, various features are incorporated into the system for aligning and orienting sperm in flow channels, as well as, for determining sperm orientation and measuring relative DNA content for analysis and / or sorting.

Improved insemination systems particularly adapted to use for sex-selected sperm sorting include systems which achieve superovulation and then multiple embryo production with sexed embryos. These systems combine with other techniques, including techniques for enhanced sheath fluid and other strategies which minimize stress on the sperm cells, and, potentially, a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial application of sperms samples as well as the resulting animals may be achieved.

Sperm in semen are sorted by fluorescence-activated cell sorting into gender-enriched populations enriched in X-chromosome or Y-chromosome bearing sperm by use of a fluorescent quantitative DNA-binding vital stain.

Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.

This disclosure relates to a device in the form of a microfluidic chip. In particular, various features are incorporated into the microfluidic chip for aligning and orienting sperm in flow channels, as well as for separating selected subpopulations of sperm.

Cell sorting methods that improve sorting efficiency and productivity by elevating sorting pressures and incorporate certain steps to help the cells better survive such elevated pressures. In the case of sperm, sorting the steps of standardizing sperm samples, staining sperm samples in a single step, calibrating a flow cytometer to place sperm in the leading edge of droplets, and changing a catch fluid distance may be incorporated individually, or in combination to help sperm better survive the sex sorting process.

This disclosure relates to a system for sorting sperm cells in a microfluidic chip. In particular, various features are incorporated into the system for aligning and orienting sperm in flow channels, as well as, for determining sperm orientation and measuring relative DNA content for analysis and / or sorting.

Methods for processing and sorting sperm are disclosed. Portions of sperm sorting or staining processes may include standardizing sperm samples by adjusting the concentration of the sperm sample to a predetermined concentration and adjusting the pH of the sample to a predetermined value. Sperm may also be stained in a single staining buffer having a DNA selective dye and a quenching dye.

This disclosure relates to methods for sorting sperm cells in a microfluidic chip. In particular, various steps are incorporated to align and orienting sperm in flow channels, as well as, to determining sperm orientation and measure relative DNA content for analysis and / or sorting.

A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

Current invention is about the sex-specific separation of sperm in high purity. For more details, this invention is about the methods for improving the separation efficiency of sperm by removing factors deteriorating separation efficiency by utilizing several parameters and adjusting few conditions for the purpose of separating sperm into X-chromosome bearing and Y-chromosome bearing groups in high purity. Current invention, for the purpose of separating X-chromosome bearing and Y-chromosome bearing sperm based on physiological characteristics, utilize the difference of sperm's nucleus width as a parameter to recognize the difference in DNA content between X and Y chromosome. Current invention is about the sex-specific separation of sperm in high purity. This invention, unlike any known conventional methods, requires neither special conditions nor special treatments, can be conducted in a more realistic environment and, as a results, sex-specific sorting of sperm can be conducted without losing viability or motility. Therefore, the limitation conferred on sorting efficiency by the motility can be overcome by our invention.

A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

This disclosure relates to a device in the form of a microfluidic chip. In particular, various features are incorporated into the microfluidic chip for aligning and orienting sperm in flow channels, as well as for separating selected subpopulations of sperm.

A method of sex sorting sperm is disclosed. The sperm may be stained with a DNA selective fluorescent dye, which fluoresces when excited, a dead quenching dye, which selectively quenches fluorescence emitted by the DNA selective fluorescent dye within the membrane of compromised sperm, and a split enhancing dye. The stained sperm may then sorted into one or more gender enriched subpopulations of viable sperm.

A microfluidic chip for sorting sperm includes a substrate, a sample channel, and a plurality of divergent channels. The sample channel is disposed in the substrate. The divergent channels are disposed in the substrate. Each of the divergent channels includes a main channel and two branch channels. The two branch channels are connected to an end of the main channel away from the sample channel. The two branch channels are disposed at two sides of the main channels respectively. The sample channel and the main channels of the plurality of divergent channels are substantially connected to each other in serial along a direction.

Improved insemination systems particularly adapted to use for sex-selected sperm sorting include systems which achieve superovulation and then multiple embryo production with sexed embryos. These systems combine with other techniques, including techniques for enhanced sheath fluid and other strategies which minimize stress on the sperm cells, and, potentially, a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial application of sperms samples as well as the resulting animals may be achieved.

The invention discloses sheep X and Y sperm sorting liquid, a sorting floating system and a sorting method. According to the disclosed scheme, sheep sperms X and Y are sorted by using a specific protein TLR7 / 8 agonist R848 "floating system". According to the technology for activating and sorting the X and Y sperms through the specific protein TLR7 / 8 of the X and Y sperms of the milk goat, the separation method is simple, the effect is good and reliable, and the X and Y sperms of the milk goat can be efficiently separated; the X sperms sorted by the method are used for in-vitro fertilization, and the proportion of female embryos reaches 80.52%; the X sperms sorted by the method are used for artificial insemination, and the proportion of female embryos reaches 88.89%.

The invention provides a sperm sorting chip, sperm detection equipment and a sperm detection method and relates to the technical field of biological detection equipment. The sperm sorting chip, the sperm detection equipment and the sperm detection method are designed for solving the problem that existing sperm sorting and detecting equipment is tedious in detection flow and high in cost. The sperm sorting chip comprises an upper plate and a lower plate. An injection hole and a suction hole are formed in the upper plate, a sorting slot is formed in a panel where the upper plate and the lower plate are attached, the sorting slot is communicated with the injection hole and the suction hole, a temperature control module is arranged on the upper plate or the lower plate and used for endowing the sorting slot with a temperature gradient, the temperature at one side, relative to the sorting slot, of the injection hole is lower than the temperature at one side, relative to the sorting slot, of the suction hole. The sperm detection equipment comprises the sperm sorting chip. The sperm detection method detects the sperm activity by means of the sperm detection equipment. The sperm sorting chip, the sperm detection equipment and the sperm detection method provided by the invention are used for detecting the sperm activity.

A sperm sorter and a sperm sorting method are provided. The sperm sorter includes an inlet chamber, a swim up sorting chamber, a divergent channel, a recycling chamber and an outlet chamber. The swim up sorting chamber is communicated with the inlet chamber. The swim up sorting chamber is communicated between the inlet chamber and the divergent channel. The divergent channel has an entrance terminal close to the swim up sorting chamber and an exit terminal away from the swim up sorting chamber. A width and a depth of the entrance terminal are respectively less than a width and a depth of the exit terminal. The recycling chamber is communicated with the exit terminal of the divergent channel. The outlet chamber is communicated with a portion of the divergent channel between the entrance terminal and the exit terminal.

This disclosure relates to methods for sorting sperm cells in a microfluidic chip. In particular, various steps are incorporated to align and orienting sperm in flow channels, as well as, to determining sperm orientation and measure relative DNA content for analysis and / or sorting.

Cell sorting methods that improve sorting efficiency and productivity by elevating sorting pressures and incorporate certain steps to help the cells better survive such elevated pressures. In the case of sperm, sorting the steps of standardizing sperm samples, staining sperm samples in a single step, calibrating a flow cytometer to place sperm in the leading edge of droplets, and changing a catch fluid distance may be incorporated individually, or in combination to help sperm better survive the sex sorting process.

A system and method for sorting sperm is provided. The system includes a housing and a microfluidic system supported by the housing. The system also includes an inlet providing access to the microfluidic system to deliver sperm to the microfluidic system and an outlet providing access to the microfluidic system to harvest sorted sperm from the microfluidic system. The microfluidic system provides a flow path for sperm from the inlet to the outlet and includes at least one channel extending from the inlet to the outlet to allow sperm delivered to the microfluidic system through the inlet to progress along the flow path toward the outlet. The microfluidic system also includes a filter including a first plurality of micropores arranged in the flow path between the inlet and the outlet to cause sperm traveling along the flow path to move against through the filter and gravity to reach the outlet.

The invention discloses a micro-fluidic chip for sorting sperms, which is used for sorting high-quality sperms from seminal fluid by utilizing the taxis characteristic of the sperms. According to the micro-fluidic chip, a substrate with a supporting function is used as a lower layer; the upper layer is made of a flexible material engraved with channels and a collecting structure, and the channels comprise a vertical sample introduction channel and a horizontal branch sample introduction channel which are distributed on the two sides of the main flow channel, a vertical buffer solution inlet channel, a vertical buffer solution outlet channel, and a vertical sample outlet channel and a horizontal branch sample outlet channel which are located on the two sides of the main flow channel. The flexible material on the upper layer and the substrate on the lower layer are tightly attached together. The sperm sorting chip has the beneficial effects that the sperm sorting chip has the advantages of simplicity and convenience in preparation, low cost, easiness in operation, high sorting efficiency and the like; besides, high-flux sperm sorting can be realized, so that the efficiency of sperm sorting is greatly improved, and the method has a wide application prospect in the field of assisted reproduction.

The invention belongs to the crossing field of combining micro-nano machining with life science and particularly relates to a sperm sorting device. The sperm sorting device provided by the invention comprises a semen cavity and a semen inflow channel communicating with a semen storage cavity; the semen cavity is divided into an upper cavity and a lower cavity by a horizontally arranged PDMS through hole membrane; the lower cavity is the semen storage cavity; the upper cavity is a semen recovery cavity; and the PDMS through hole membrane is prepared by micro-nano machining. For the sperm sorting device provided by the invention, a filter membrane adopted by the sperm sorting device is the PDMS through hole membrane prepared by a micro-nano machining technology, and the thickness, the pore diameter, the uniformity and the porosity of the PDMS through hole membrane prepared by the method are highly controllable, so that the sperm sorting device provided by the invention can realize greatimprovement on the sperm recovery efficiency through a mode of increasing the porosity of the arranged PDMS through hole membrane.

The present invention provides a sperm sorting chip and a method for sorting sperm using the same. Said sperm sorting chip includes: a flow channel structure sequentially configured with a gradually diverging flow field region, a main flow channel, and a gradually converging main flow channel intercommunicated with each other from a first side end to a second side end; a fluid injection port, a semen injection port, and a semen extraction port separately located at the first side end and communicated with a main input channel of the gradual diverging flow field region; and a waste fluid outlet located at the second side end and communicated with the gradually converging main flow channel. The gradually diverging flow field region further includes a plurality of sub-input channels derived from the main input channel and converged into the main flow channel, and the plurality of sub-input channels have a gradually widening channel width at the junction with the main flow channel. By contrast, the gradually converging main flow channel has a gradually narrowing channel width toward the waste fluid outlet.

A sperm sorting apparatus and a sperm sorting method are provided. The sperm sorting apparatus includes a medium chamber, a waste chamber and a sorting channel communicated with and extending along a first direction in between. The medium chamber is configured to contain a medium solution. The waste chamber disposed aside the medium chamber is configured to contain a residual solution after sorting. The residual solution includes low motility sperms and / or dead sperms. The sorting channel is configured to be inserted with a sperm sample, such that sperms in the sperm sample are sorted in corresponding to a medium solution flow from the medium chamber. The sorting channel has first and second portions. The first portion is closer to the medium chamber than the second portion. A width of the first portion measured along a second direction is greater than a critical dimension, ranging from 200 μm to 400 μm.

The invention discloses a sperm sorting bionic system, which comprises a bionic fallopian tube flow channel used for allowing sperms to flow therein, one end of the bionic fallopian tube flow channel is a flow channel inlet, the other end of the bionic fallopian tube flow channel is a flow channel terminal, and the bionic fallopian tube flow channel is used for placing at least one ovum so that the sperms can reach the flow channel terminal to be combined with the ovum; the bionic fallopian tube flow channel is fixed above the base; the electrode assembly is arranged between the bionic fallopian tube flow channel and the substrate and used for generating adsorption voltage, and sperms carrying X chromosomes can be adsorbed when the electrode assembly outputs the adsorption voltage. The electrode assembly is adopted, adsorption voltage is generated when sperms flow through the electrode assembly, a large number of sperms carrying different specific chromosomes can be screened, the sorted sperms can be allowed to be combined with ova at the flow channel terminal, high efficiency, rapidness and high accuracy are achieved, and the quality of the sperms cannot be damaged.