Detection method for inhibiting reduction of spermatogenesis of DR5 under oxygen deficit by VHA

Abstract

The invention belongs to the technical field of inhibition of hypoxia spermatogenesis reduction, and discloses a detection method for inhibition of hypoxia spermatogenesis reduction of DR5 by VHA. VHAgene knockout mice and C57BL / 6 mice are used as research objects, and a hypoxia spermatogenesis reduction model is constructed through hypoxia induction. A seminiferous tubule seminiferous cell of anexperimental mouse is separated to serve as a research object. Hypoxic in-vitro stimulation is conducted, and the regulating effect and the molecular mechanism of VHA for inhibiting seminiferous cellexpression DR5 are verified through western blot, immunocytochemistry and qPCR technologies. According to the detection method for inhibiting DR5 spermatogenesis reduction under oxygen deficit by VHA, which is provided by the invention, the effect of hypoxic inhibition of VHA in hypoxic spermatogenesis reduction and the molecular mechanism of hypoxic inhibition of VHA in inhibition of DR5 expression are clarified, and a necessary theoretical basis and a new treatment strategy are provided for treatment of hypoxic spermatogenesis reduction.

Description

technical field [0001] The invention belongs to the technical field of inhibiting the reduction of sperm production under hypoxia, and in particular relates to a detection method for VHA inhibiting the reduction of sperm production under hypoxia by inhibiting DR5. Background technique [0002] At present, the closest prior art: In recent years, more and more people have migrated along the Qinghai-Tibet Railway, and the plateau anoxic (hypoxic hypoxia) environment has a significant negative impact on the reproductive function of male immigrants. A large number of studies on human and rat Decreased spermatogenesis has been reported. The regions in the world where there are more studies on plateau male reproduction are along the Andes in South America, along the Alps in Europe, and along the Qinghai-Tibet Plateau in my country. Most of the literature studies the reduction of sperm production from the perspective of local hypoxia. For example, in some male diseases such as varic...

AI Technical Summary

Problems solved by technology

[0003] In summary, the existing problems in the prior art are: the specific mechanism of the hypoxic spermatogenesis reduction (HSR) and the exact regulatory mechanism of hypoxia-induced spermatocyte apoptosis in the prior art are still unclear. clear

[0004] The difficulty of solving the above technical problems: the collection of semen from clinical patients involves patient privacy and needs to be reviewed by a strict medical ethics committee. Volunteers must be recruited as the control group of the experiment, and the relevant procedures are cumbersome

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