114 results about "Swine plague" patented technology

The invention discloses a four-gene-deletion weak-toxin strain for African swine fever viruses. The weak-toxin strain is the four-gene-deletion weak-toxin strain for an African swine fever virus SY18separation strain, and the following gene function protein is deleted: CD2v gene coding products and three multigene family genes( MGF360-12L, MGF360-13L and MGF360-14L ) coding products. The invention further discloses an application of the weak-toxin strain of the African swine fever viruses to preparation of vaccines for preventing or treating African swine fever. The weak-toxin strain of the African swine fever viruses can provide complete immunoprotection effect on attack of ASFV parent toxin strains, is high in safety, and is suitable for being used as vaccine candidate strains for preventing the African swine fever.

The invention discloses an antibacterial antivirus Chinese medicinal composition for the animal, comprising the following main medicament ingredients: scutellaria extract, honeysuckle extract, isatis root extract and glycyrrhiza extract. the pharmaceutical composition of the invention can be made into premix, injection, oral liquid or granula, also can be made into feedstuff premix adding into the feedstuff. the chinese medicinal composition of the invention is a pure Chinese medicinal compound preparation which has the antibacterial, anti-inflammation, antivirus and immunity-reinforced functionsand wide application range, can effectively prevent and treat various livestock and poultry diseases caused by the virus and the bacteria, such as piggyyellow-white dysentery, swine plague, infectious pleuropneumonia, asthma, canker, circovirus, swine influenza, white diarrhea, newcastle disease, bursa of Fabricius, egg drop syndrome, duck serositis and the like, and has fast effect and remarkable curative effect.

The invention discloses a traditional Chinese medicine for treating respiratory diseases and promoting growth for swine and a preparation method thereof. The traditional Chinese medicine is composed of plantain herb, herba patriniae, hindu datura flower, perilla leaf, trichosanthes kirilowii maxim and gelsmium elegans. The traditional Chinese medicine of the invention can effectively prevent and treat livestock respiratory diseases, such as swine enzootic pneumonia, infectious pleuropneumonia of swine, porcine reproductive and respiratory syndrome (PRRS), swine plague, swine influenza and thelike; meanwhile, the traditional Chinese medicine of the invention can improve animal productivity and promote animal growth. The traditional Chinese medicine of the invention has wide raw material resources, is safe and environmentally-friendly and has the advantages of simple preparation technology, low production cost and the like.

The invention discloses a fluorescence quantitative PCR detection reagent for African swine fever virus, and a preparation method and the application thereof. A set of specific primers and Taqman probes are designed and synthesized to be used for detecting ASFV P54 in relevant porcine products. A standard curve drawn in the invention provides a standard for the quantitative detection of ASFV P54. The invention establishes a fast and simple real-time fluorescence quantitative PCR detection system with strong specificity and high flexibility. The detection time is only several hours, and the detection lower limit can be 15 copies. The invention can be applied to the diagnosis and quarantine technology towards the imported relevant porcine products at port, and the invention provides reliable and effective technical condition for the import quarantine work of the country without ASF.

The invention discloses an indirect ELISA kit for detecting African swine fever virus (ASFV) antibody, and a use thereof, and belongs to the technical field of biology. The kit is characterized by: adopting prokaryotic expressed recombined P54 protein as coating antigen; detecting antibody against to the ASFV in swine serum according to an indirect ELISA principle. The coating antigen in 96-well plates of the kit is the prokaryotic expressed recombined P54 protein which has good antigenicity. The enzyme-linked immunospecific assay kit provided by the present invention comprises the 96-well plates coated by the P54 protein, positive control, negative control, horseradish peroxidase-labeled rabbit anti-porcine IgG polyclonal antibody, a concentrated cleaning solution, a serum dilution, a TMB substrate indicator and a stop buffer. The kit provided by the present invention can be provided for screening mass samples. In addition, the main reagents in the kit are provided in a working fluid manner such that the reagents are used conveniently.

The invention discloses a feed for treating swine plague and a preparation method of the feed. The feed comprises corns, soybean meal, peanut meal, wheat bran, mountain flour, fish meal, sweet potato flour, kelp powder, vitamin E and a traditional Chinese medicinal additive. The feed has the beneficial effects that the feed provided by the invention comprises the traditional Chinese medicinal additive which is mainly used for clearing heat, detoxifying, ventilating the lung and resolving phlegm and assists in soothing the throat and diminishing inflammation to relieve pain; under the combined action, the traditional Chinese medicinal additive is matched with other raw materials, so that the feed has the advantages of obvious treating effect for treating the swine plague of pigs, high curing rate, poor side effect, difficulty in reappearing and low cost.

According to the invention, the cellular immune antigen dominant epitopes of four important structural proteins p30, p54, p72 and CD2v of ASFV are screened, and the corresponding enzyme-linked immunospot test methods are established. The screened polypeptides have high amino acid sequence conservation, can effectively induce ASFV specific cellular immune response, and can be used as candidate immunogens for ASFV epitope carrier vaccines. The T cell epitope polypeptides of ASFV structural proteins obtained by the invention are screened, and the corresponding ELISPOT detection methods are established. The invention provides a powerful tool for efficacy evaluation of the ASFV candidate vaccines, and also lays a foundation for the research and development of ASFV polypeptide carrier vaccines.

The invention provides a feed composition for treating fowl pox. The composition contains traditional Chinese medicine composition additives and a feed carrier, wherein the traditional Chinese medicine composition additives comprise atractylodes rhizome, gentian, radix arnebiae, baical skullcap root, semen coicis, kudzuvine root, giant knotweed rhizome, radix bupleuri, honeysuckle, areca seed, common bletilla rubber, sweet wormwood herb, cyrtomium rhizome, dangshen, largehead atractylodes rhizome, coptis root, sanqi, common andrographis herb, radix astragali, Chinese angelica, isatis root and bark of Japanese cinnamon. Adopted to treat the swine suffering from swine plague, the feed composition has the advantages of obvious curative effect, low side effect, high cure rate, avoidance of easy relapse and the like.

This invention supplies a series of production techniques of gene recombination live vaccine of swine virus contagion with canine ó� adenovirus as carrier and finished goods. The viral live vectors vaccine takes swine important virus zymad protective antigens gene as object gene, which are chosen from HCV-E1, E2 gene, FMDV-VP1íóVP2íóVP3íóVP4 gene, TGEV-SíóNíóM gene, PEDV-SíóNíóM geneú¼SIV-HAíóNA geneú¼RV-GíóN gene, etc. The produced vaccines contain recombined swine influenza virus HA gene adenovirus carrier live vaccine, swine plague virus E2 gene adenovirus carrier live vaccine and recombination swine AsiaI foot-and-mouth disease virus VPI gene adenovirus carrier live vaccine. Recombination virus has good inheritance stability, and vaccine immunization can induct pig develop differential antiviral neutralization antibody. It has good immune protection effect and has no toxic side effect. The goods are facilitating for preserve and transportú”it has long storage life and simple technics, and it fits for commercial manufacture.

The invention discloses a florfenicol compound composition and a preparation method thereof. The composition is composed of florfenicol, azithromycin, tylosin, propylene glycol, dimethyl formamide, alpha-pyrrolidone, anhydrous ethanol and polyvinylpyrrolidone (PVP). The preparation method of the composition comprises steps of: 1) adding dimethyl formamide to florfenicol, stirring and dissolving; and sequentially adding propylene glycol, alpha-pyrrolidone and anhydrous ethanol, and stirring to mix evenly; 2) adding azithromycin and tylosin, and stirring to mix evenly; 3) heating with stirring at 40 DEG C, adding polyethylene pyrrole pyrrolidone, dissolving and continuing to stir for 15min; and 4) filtering the obtained solution, inflating nitrogen and encapsulating to obtain the florfenicol compound composition. The compound composition provided by the invention is mainly used in the treatment of swine respiratory diseases, and especially has specific long-term treatment effect and no recurrence on respiratory diseases, such as infectious pleuropneumonia, Haemophilus parasuis, swine plague and asthma.

The invention provides a special kit for detecting highly pathogenic porcine reproductive and syndrome virus mutation strain. The highly pathogenic porcine reproductive and syndrome virus mutation strain is detected by self-designing specific primers PSX1 / PSX2, extracting virus RNA and carrying out reverse transcription into cDNA and utilizing a PCR method; the size of the estimated amplification segment of an amplification mutation strain is 404bp and an amplification traditional PRRSV amplification segment is 494bp. Compared with the prior art, the special kit has strong specificity and sensitivity, and the coincidence rate can achieve more than 90 percent compared with a virus separation and IPMA method. The amplification results of the primers to hogcholera virus, pseudorabies virus and porcine parvovirus are negative, which can distinguish a NSP2 mutation strain and a traditional strain; the method can detect the PRRSV with 12.8ng / L, thus the invention can be widely used for the clinical detection of the highly pathogenic porcine reproductive and syndrome virus mutation strain.

The invention discloses a compound feed of a weaned pig, which comprises the following components in parts by weight: 60-65 parts of corns, 15-20 parts of soya bean meals, 3-6 parts of whey powder, 2-5 parts of fish meal, 2-5 parts of soycomil, 2-5 parts of wheat middling, 2-3 parts of glucose, 4 parts of pig premix and 0.05-0.5 part of aloe polysaccharide additives. The compound feed has the advantages that: the feed has high technological content and comprehensive and balanced nutrition, is rich of various nutrition components needed by growth of the weaned pig, can improve the immunity of the weaned pig and enhance the disease-resistant capacity; the product has good palatability and high feed return, and the average daily feed intake of the pigs weaned within four weeks is 800-1000g, the average daily gain is 400-600g and the feed meat ratio is 1.7-1.8. The swine plague antibody titer of the weaned pig is increased and the content of cell factors in serum can be adjusted.

The invention belongs to the technical field of bioengineering, and particularly relates to a gene-deleted attenuated African swine fever virus strain with innate immune suppression and application. The invention finds that the ASFV MGF-505-7R can inhibit generation of interferon; an ASFV MGF-505-7R gene is deleted in an African swine fever original virulent strain to obtain the attenuated Africanswine fever virus strain with higher safety, thus providing a theoretical basis and practical reference for successful preparation of an African swine fever vaccine in the future. Further, a researchworker can finally prepare safe and effective African swine fever vaccine candidate strains by knocking out the ASFV MGF-505-7R and one or more disclosed virulence genes (such as CD2V, MGF360-12L, MGF360-13L, MGF360-14L, MGF360-505R and the like) simultaneously.

The invention relates to an anti-African swine fever virus p54 protein monoclonal antibody and a preparation method and application thereof. According to the invention, polypeptide capable of simulating conservative p54 protein NTD is designed and synthesized, and the polypeptide is coupled with BSA to serve as immunogen, an anti-ASFVp54 protein monoclonal antibody is prepared by immunizing a BALB / c mouse through an immunological method, the obtained antibody can specifically identify and bind to an N-terminal region of p54 protein, and the recognition region (aa 1-29) is different from the existing commercial monoclonal antibody. The invention provides gene sequences of a heavy chain variable region and a light chain variable region of the antibody. On this basis, the monoclonal antibodyof the invention can be obtained by adopting a conventional genetic engineering method or a protein engineering method. The monoclonal antibody is high in specificity and high in sensitivity, capableof specifically reacting with the ASFV HLJ / 18 virus strain, without reaction with CSFV, PCV2, PRRSV, PEDV, PRV and other swine viruses, thus laying a foundation for the research of ASFV etiology and pathogenesis and the clinical detection research of ASFV pathogens.

The invention discloses a pig plague detecting method and system, and belongs to the field of livestock breeding application. The method comprises the following steps: acquiring the body temperature,movement, the sulfide concentration and the hemoglobin concentration change of a pig in the T time period by using a smart ear tag, and transmitting the data to a management terminal; allowing the management terminal to analyze the data to form the actual body temperature, the actual movement mount, the actual sulfide concentration, the actual hemoglobin concentration change curve of the pig, analyzing each curve to determine if the pig is in the pig plague state; and allowing the management terminal to inform the staff to check the pig if so. The system includes the management terminal capable of performing data analysis and outputting results, and the smart ear tag capable of detecting the body temperature, the movement amount, the sulfide concentration, and the hemoglobin concentrationand communicating with the management terminal. The pig plague detecting method and system are conducive to the early detection of swine plague, preventing a large number of infections, effectively reducing losses, and also reducing the risk of workers infected with swine erysipelas.

The invention discloses PCR primers, a PCR detection method and a PCR detection kit for detecting and identifying atypical porcine pestivirus (APPV). By referring to and comparing the genomic sequence of the atypical porcine pestivirus, based on the conserved sequence of the cap protein of the atypical porcine pestivirus, a pair of PCR primers for detecting the atypical porcine pestivirus is designed, and the sequences of the forward primer and the reverse primer of the PCR primers APPV-F / R are shown as SEQ ID NO.1- SEQ ID NO.2 in sequence; the PCR detection method and the PCR detection kit for detecting the atypical porcine pestivirus are further provided. The primers and the detection method can detect and identify the atypical porcine pestivirus fast, conveniently and efficiently, specificity is good, and sensitivity is high; moreover, operation of the detection method is easy and efficient, and the detection method brings convenience to clinical detection and epidemiological investigation, and has a broad application prospect.

The invention discloses a pharmaceutical composition for preventing and treating chronic swine plague and a preparation method of the pharmaceutical composition. The pharmaceutical composition comprises the following raw Chinese medical materials: gypsum rubrum, jasmine, dried radix rehmanniae, cacumen biotae, moutan bark, rhizoma phragmitis, radices trichosanthis, lophatherum gracile, dayflower, phoenix-tail fern, polygonum aviculare, dandelion, lotus leaf, lotus plumule, radix ophiopogonis, fructus forsythia, radix scrophulariae, liquorice, Chinese eriosema root, dicliptera chinensis, bryoniifolia, adenophora trachelioides maxim., Indian melothria root, cynanchum thesioides, trichosanthes kirilowii maxim, tuberous sword fern tuber, giant clam meat and yam rhizome. The pharmaceutical composition mainly takes effects of clearing away heat and toxic materials, cooling blood and promoting the secretion of body fluid and also has auxiliary functions of invigorating spleen and supplementing qi, so as to reconcile yin and yang, and qi and blood of pigs with chronic swine plague to finally treat swine plague; the pharmaceutical composition for preventing and treating chronic swine plague has the advantages of short treatment course, relatively good effect, low residual quantity and good environment friendliness and simultaneously is capable of enhancing the immunity and the disease resistance of pigs.

The invention provides a traditional Chinese medicine composition for treating swine plague. The composition comprises: baikal skullcap root, brucea javanica, pinellia ternate, honeysuckle flower, polygonum cuspidatum, bletilla rhizome, radix codonopsis, cordate houttuynia, pseudo-ginseng, radix astragali, radix scrophulariae, radix puerariae, gastrodia elata and licorice. The traditional Chinese medicine composition has substantial cure effect on pigs suffering from swine plague, and has the advantages of simultaneously treating principal and subordinate symptoms, reinforcing the healthy qi and eliminating the pathogenic factors, and being high in cure rate, none in toxic and side effects, hard to recurrence, without hazard or drug resistance, low in cost, improved in organism immunity and the like.

The invention discloses an antibacterial antivirus Chinese medicinal composition for the animal, comprising the following main medicament ingredients: scutellaria extract, honeysuckle extract, isatis root extract and glycyrrhiza extract. the pharmaceutical composition of the invention can be made into premix, injection, oral liquid or granula, also can be made into feedstuff premix adding into thefeedstuff. the chinese medicinal composition of the invention is a pure Chinese medicinal compound preparation which has the antibacterial, anti-inflammation, antivirus and immunity-reinforced functionsand wide application range, can effectively prevent and treat various livestock and poultry diseases caused by the virus and the bacteria, such as piggyyellow-white dysentery, swine plague, infectious pleuropneumonia, asthma, canker, circovirus, swine influenza, white diarrhea, newcastle disease, bursa of Fabricius, egg drop syndrome, duck serositis and the like, and has fast effect and remarkable curative effect.

The invention discloses soluble compound doxycycline hyclate powder for animals and a preparation method thereof, which aim to provide compound doxycycline hyclate powder which is used for treating the mixed infection of swine plague mycoplasma pneumonia, has quick effect and can treat both symptoms and root causes, lighten stress reactions when pigs fall ill and improve the weight growth ratio, and a preparation method which has simple process and is easy to realize. The soluble powder comprises the following components by the weight percent: 1-20 of doxycycline hyclate, 0.5-5 of ofloxacin, 5 of aminophylline and the balance of anhydrous dextrose. The soluble compound doxycycline hyclate powder adopts antibiotics, antibacterial drugs and antiasthmatic drugs in a reasonable proportion so as to have multiple actions, can quickly ease clinic symptoms caused by the swine plague and the mycoplasma pneumonia, reduce damages caused by the two diseases to organisms, quicken the recovery, kill pathogens fundamentally, reduce the probability of the drug resistance of pathogenic microorganisms and treat both the symptoms and the root causes, and has quick effect.

The invention discloses a pasteurella bacteriophage vB_PmuP_PS02 and a bacteriophage composition and application thereof. The pasteurella bacteriophage vB_PmuP_PS02 is collected in the China General Microbiological Culture Collection Center on May 15, 2020, and has a collection number of CGMCC No.19972. The bacteriophage and the bacteriophage composition obtained by compounding the bacteriophage not only can be used for preparing medicines for preventing and treating various livestock diseases caused by pasteurella infection, especially have remarkable effects on swine plague and swine progressive atrophic rhinitis caused by swine pasteurella diseases, but also can be used for preparing swine feed additives, environment and feed disinfectants and the like. The bacteriophage and the bacteriophage composition thereof are safe to use and free of side effects, and the problems of antibiotic residues caused by traditional use of antibiotics and induction of drug-resistant pasteurella are effectively avoided.

The invention provides traditional Chinese medicine feed for treating swine plague. The traditional Chinese medicine feed for treating swine plague comprises white atractylodes rhizome, root of kudzu vine, Chinese angelica, radix bupleuri, lithospermum, safflower, honeysuckle, scutellaria baicalensis, pinellia ternate, brucea javanica, sculellaria barbata, rhizoma bletillae, codonopsis pilosula, fructus forsythiae, houttuynia cordata, milkvetch, schisandra chinensis, radix scrophulariae, gastrodia elata, glossy privet fruit, polygonum cuspidatum and liquorice. The Chinese medicine composition is reasonable in composition, can achieve synergistic interaction, has a stronger antiviral effect and has the advantages of obvious curative effect, treatment on both symptoms and root causes, strengthening of the body resistance for eliminating evil, high curative ratio, no toxic or side effect, difficult recurrence, no phytotoxicity, no drug resistance, low cost and improvement on immunity of an organism.

The invention belongs to the technical field of veterinary medicines, and specifically relates to a preparation method for compound tilmicosin enteric-coated granules. The preparation method sequentially comprises the steps of: preparing materials, mixing the materials, granulating, preparing a coating solution, coating and the like, wherein the raw materials of the granules are tilmicosin, colistin, gatifloxacin, a sweetening agent, a flavouring agent, bromhexine hydrochloride, oral glucose, corn starch, saccharose powder, dextrin, talcum powder and starch slurry; the raw materials of the coating solution are HPMC, Tween-80, polyethylene glycol 6000, talcum powder and ethanol. The method disclosed by the invention enables the tilmicosin effective ingredient not to be broken in gastric acid, and to safely arrive at intestinal tracts to be absorbed; the obtained compound tilmicosin enteric-coated granules are capable of preventing and treating respiratory diseases such as mycoplasma diseases, pleuropneumonia and swine plague, capable of reducing the occurrences of bacterial diseases such as diarrhoea and salmonellosis, and capable of increasing the feed conversion.

The invention provides a feed composition for treating swine plague. The composition contains traditional Chinese medicine composition additives and a feed carrier, wherein the traditional Chinese medicine composition additives comprise honeysuckle, lophatherum herb, red peony root, pinellia tuber, white paeony root, giant knotweed rhizome, dandelion, radix bupleuri, dwarf lilyturf tuber, common bletilla rubber, dangshen, figwort root, gentian, semen coicis, radix astragali, baical skullcap root, gypsum, liquorice and inner membrane of chicken gizzard. Adopted to treat the swine suffering from swine plague, the feed composition has the advantages of obvious curative effect, low side effect, high cure rate, avoidance of easy relapse and the like.

The invention discloses a traditional Chinese medicine for treating respiratory disease of livestock and a preparation method thereof. The traditional Chinese medicine comprises five traditional Chinese medicinal materials of herba violae, turkey corn, aster, herba ephedra and radix scutellariae. The traditional Chinese medicine can be effectively used for treating diseases of swine enzootic pneumonia, swine contagious pleuropneumonia, porcine reproductive and respiratory syndrome, swine plague, swine influenza, infectious bronchitis of chicken, infectious laryngotracheitis of chicken and thelike, and has the advantages of environment protection without toxic and side effects, simple preparation process, low production cost and the like.

The invention relates to an animal feed, and in particular relates to an animal feed capable of preventing respiratory diseases of animals. The animal feed is characterized by comprising the following materials in percentage by mass: 25-40 percent of energy feeds containing corns, wheat middling, broken rice and wheat, 20-50 percent of protein feeds containing greens, soybeans and sesame cookies, and 5-10 percent of traditional Chinese medicinal feeds containing dried radix rehmanniae, folium isatidis, liquorice, Sichuan rheum officinale, radix scrophulariae and fructus forsythiae. Due to the addition of the traditional Chinese medicinal feeds for treatment into the feed, the animal feed is mainly used for treating heat toxins, high fever, coma, viral respiratory diseases of pigs, enzootic pneumoniae of pigs, swine plague, swine influenza and post-weaning multisystemic wasting syndrome of weaned piglets, has the effects of invigorating spleen to promote digestion and detoxicating and destroying intestinal worms, and can improve the meat quality of the animals, enhance the disease resistance and effectively suppress the respiratory diseases.

The invention belongs to the technical field of biology, and particularly relates to application of African swine fever virus E120R protein as an immunosuppressant and construction of an immunosuppression site knockout strain. The invention firstly discovers that the African swine fever virus E120R protein can inhibit activation of IFN-beta induced by cGAS / STING and poly (dA: dT), inhibit mRNA expression of IFN-beta induced by cGAS / STING and poly (dA: dT) and downstream factors ISG56 and ISG15 thereof, inhibit phosphorylation of IRF3 induced by cGAS / STING and poly (dA: dT), has a relatively strong immunosuppression effect, and can be used as an immunosuppressant; and secondly, the invention discovers an immunosuppression site of the African swine fever virus E120R protein, and constructs an African swine fever recombinant virus with the knockout of the immunosuppression site of the E120R protein through a genetic engineering means, the natural immunity of the African swine fever recombinant virus is significantly enhanced, and the African swine fever recombinant virus can be used as a recombinant vaccine strain, can promote early immune response, induce the production of high-levelantibodies, improve the biological safety and immune protection efficacy, and has good application prospects.

The invention discloses drug for curing swine plague and a preparation method thereof. The drug comprises root of straight sophora, bovista, goldthread root, cape jasmine, scutellaria root, pollen, immature bitter orange, rhubarb, figwort root, Chinese gentian, akebia stem, forsythia fruit, balloonflower, compound of glauber-salt and liquorice, silkworm larva, cyathocline purpurea, semen lepidii, perilla fruit, Zhejiang fritillary bulb, radix et rhizoma thalictri, areca and aristolochia fruid. The drug has the benefits that the drug is based on clearing away heat and toxic material, ventilating the lung and resolving phlegm, also has the effects of throat expediting and relieving inflammation and alleviating pain and is used for curing swine plague, and compared with traditional Chinese medicine and Western medicine in the prior art, the drug has the advantages of obvious curative effect on curing pigs with swine plague, high cure rate, small side effect, uneasiness in relapse and low cost.

The invention discloses a functional compound feed for preventing and treating chronic swine plague and a preparation method of the functional feed. The functional feed comprises the following raw materials: wheat bran, corns, wheat, lotus root starch, millet, kelp, asparagus, grapefruits, Chinese wax gourd peels, edible amaranth, soybean meal, tomatoes, loofah, lotus seeds, oyster mushrooms, vitamin premix and microelement premix. The functional compound feed disclosed by the invention has the beneficial effects of containing the traditional Chinese medicine additives for clearing away heat and toxic materials, cooling blood and promoting the secretion of saliva or body fluid, being used for strengthening the body resistance to eliminate pathogenic factors and having the advantages of relatively short course of treatment, relatively good course of treatment, no drug residue, low cost and price and the like; the functional feed has the capabilities of enhancing the immunity and disease resistance of swine while achieving harmony between yin and yang as well as qi and blood of the swine, short course of treatment, relatively good effect and low residual mount, and is environmentally friendly and is free of pollution.