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98 results about "Colletotrichum sp." patented technology
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Polynucleotides and methods for making plants resistant to fungal pathogens
InactiveUS20060223102A1Microbiological testing/measurementOther foreign material introduction processesAnthriscus caucalisPolynucleotide
This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.
Owner:PIONEER HI BRED INT INC +2
Bacillus methylotrophicus F7 and application thereof
PendingCN106754489AStrong antagonistic effectGood biological control effectPlant growth regulatorsBiocideRalstonia solanacearumPlant growth
The invention discloses bacillus methylotrophicus F7 and an application thereof. The invention aims at providing a strain which has a plurality of beneficial properties, namely producing siderophore, cellulase and protease, producing auxin, producing ammonia and the like; the strain not only can be used for effectively inhibiting various pathogenic bacteria such as ralstonia solanacearum, fusarium oxysporum f.sp.cubense, colletotrichum gloeosporioides, fusarium oxysporum f.sp cucumerinum but also can promote plant growth. In accordance with the technical scheme, the bacillus methylotrophicus F7 is preserved in China General Microbiological Culture Collection Center (CGMCC) on February 1, 2016, with preservation number of CGMCC No.12124; in addition, five surfactin homologs, six fengycin homologs and three iturin homologs, which have an antibacterial effect, are extracted from fermentation liquid of the bacillus methylotrophicus F7; and the bacillus methylotrophicus F7 belongs to the field of agricultural biotechnology.
Owner:SOUTH CHINA AGRI UNIV
Application of bacillus amyloliquefaciens in preventing and controlling fungal disease of plants
The invention discloses application of bacillus amyloliquefaciens in preventing and controlling fungal disease of plants. The application has the advantages that found by study, the bacillus amyloliquefaciens, especially bacillus amyloliquefaciens BSY3 separated and screened from African marigold by an inventor, has obvious antagonistic function on phellinus noxius, pyricularia cannaecola hashioka, sclerotinia sclerotiorum, colletotrichum sp., pestalotiopsissp, fusarium oxysporum f.sp.cubense and the like, and has obvious biological control function and potential application value on preventing and controlling of the fungi diseases due to pathogenic bacteria; the bacillus amyloliquefaciens comes from endophytic bacteria in plants, so that compared with the traditional chemical agent preventing and control method, the safer biological control effect on the fungi diseases is realized; because the bacillus amyloliquefaciens stays in the plant for a long time, the survival relationship with harmonious coexistence and stable development is established with the plant; compared with the chemical agent, the biocontrol function can be more effectively and stably realized.
Owner:广州市林业和园林科学研究院
LAMP (loop-mediated isothermal amplification) primer composition for detecting colletotrichum gloeosporioides and application of LAMP primer composition
ActiveCN104611428AImprove featuresStrong specificityMicrobiological testing/measurementMicroorganism based processesColletotrichum sp.Cell biology
The invention discloses LAMP (loop-mediated isothermal amplification) primer composition for detecting colletotrichum gloeosporioides and an application of the LAMP primer composition. A GS (glutamine synthetase) gene is used as a target gene, primers which have high specificity and high sensitivity and can be used for LAMP detection of the colletotrichum gloeosporioides are designed and screened out, and the LAMP primer composition consists of a forward outer primer GS-F3 shown in SEQ ID NO.2, a reverse outer primer GS-B3 shown in SEQ ID NO.3, a forward inner primer GS-FIP shown in SEQ ID NO.4, a reverse inner primer GS-BIP shown in SEQ ID NO.5, a loop primer GS-LF shown in SEQ ID NO.6 and a loop primer GS-LB shown in SEQ ID NO.7. The primer composition is mainly used for quickly detecting the colletotrichum gloeosporioides, and only 2 hours are spent on detection each time.
Owner:NANJING AGRICULTURAL UNIVERSITY
Primer for detecting orchid colletotrichum gloeosporioides molecules and quick detection method
InactiveCN102534017AStrong specificityImprove practicalityMicrobiological testing/measurementDNA/RNA fragmentationGel electrophoresisPlantlet
Owner:INST OF PLANT PROTECTION FAAS
Novel thiosemicarbazone compound and preparation method thereof
ActiveCN102219769AEasy to prepareEasy to operateOrganic chemistryFungicidesFusarium oxysporumColletotrichum acutatum
The invention mainly provides a thiosemicarbazone compound which has an inhibition effect on fusarium oxysporum, fusarium moniliforme, fusarium solani and colletotrichum and a preparation method thereof. The chemical structural formula of the compound is shown in the specification. According to the invention, a novel way for preventing and controlling plant diseases caused by fusarium oxysporum, fusarium moniliforme, fusarium solani and colletotrichum is provided; and the preparation method is simple and convenient and has strong operability.
Owner:福建省农业科学院农业生物资源研究所
Bacillus licheniformis 201 and use thereof
InactiveCN101486978AAntagonisticEnhanced inhibitory effectBiocideBacteriaBiotechnologyBacillus licheniformis
The invention discloses a Bacillus licheniformis 201, which is preserved in China Center for Type Culture Collection that is located in Wuhan University, Logosun, Wuchang, Wuhan, Hubei, and with CCTCC NO. of M207092. The invention simultaneously discloses a preparation method and applications of the Bacillus licheniformis 201, which has strong bacteriostasis of banana wilt fusarium, wide bacteriostasis spectrum and antagonism to various pathogenic fungi including banana wilt fusarium, watermelon wilt fusarium, tomato wilt fusarium, banana colletotrichum, gloeocystidium colletotrichum, corn leaf spot curvularia, cotton verticillium lecanii, gibberella zeae, and the like, and the Bacillus licheniformis 201 has high control efficiency, good environmental security and good development and application prospect.
Owner:SOUTH CHINA AGRI UNIV
Huperzia serrata endophytic fungi and its use in production of huperzine a
ActiveCN103103134ASolve bottlenecksSave natural resourcesFungiMicroorganism based processesBiotechnologyNatural resource
The invention relates to the technical field of microbes and especially relates to huperzia serrata endophytic fungi and its use in production of huperzine a. The huperzia serrata endophytic fungi are named as Colletotrichum sp. SY-1, are preserved in the China center for type culture collection (CCTCC) on August 19, 2011, and have the preservation number CCTCC M2011293. Through fermentation, the huperzia serrata endophytic fungi can produce a huperzine a compound. Through utilization of characteristics of microbe-based huperzine a production and the modern fermentation breeding technology, huperzine a industrial production is realized; bottleneck problems of huperzine a exploitation are solved; and the endangered huperzine a natural resource is protected.
Owner:吴水生
Bacillus subtilis Baisha2C for restraining plant pathogenic fungi
The invention belongs to the field of biocontrol strain excavation and utilization, in particular to bacillus subtilis Baisha2C for restraining plant pathogenic fungi, and the preservation number of the bacillus subtilis Baisha2C is CGMCC No.10963. The invention further discloses molecular biological characteristics, physiological-biochemical characteristics and a cultivation method of the bacillus subtilis Baisha2C, and the bacillus subtilis Baisha2C has activity for restraining various kinds of the plant pathogenic fungi, such as fusarium colletotrichum, colletotrichum gloeosporioides and fusarium.
Owner:ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
Sugarcane endophytic bacillus and application thereof
ActiveCN110791448AExclude the risk of pathogenicityAvoid negative effectsBiocideBacteriaBiotechnologyEcological environment
The invention belongs to the field of biological pesticides, and discloses a sugarcane endophytic bacillus amyloliquefaciens RGB15 strain and an application thereof. The bacillus amyloliquefaciens CGB15 strain is preserved in the Guangdong Province Microbial Culture Collection Center on October 17, 2019, and the preservation number is GDMCC No: 60742, and the preservation address is the 5th floorof No.59 building of No.100 courtyard of the Xianlie Middle Road of Guangzhou city. The bacillus amyloliquefaciens RGB15 strain has a strong inhibition effect on ustilago scitaminea, pyricularia grisea, peronophythora litchii, colletotrichum litchii and banana fusarium oxysporum, and has an excellent biological control effect. A microbial preparation prepared from the strain is simple in preparation and use method, has broad-spectrum disease resistance, has little influence on the ecological environment, does not influence the crop quality, and has an excellent application prospect.
Owner:SOUTH CHINA AGRI UNIV
Method for quickly detecting latent infection and medication of strawberry anthracnose in seedling stage
ActiveCN108085410AImprove practicalityGet rid of dependenceMicrobiological testing/measurementFragariaLoop-mediated isothermal amplification
The invention discloses a loop-mediated isothermal amplification (LAMP) detection method for detecting strawberry anthracnose in the seedling stage and an LAMP primer used in the method; and the method can be used for quickly detecting whether strawberry seedlings carry germs or not on the site. The invention also discloses an LAMP detection method for detecting anti-QoI germicide G143A genotype strawberry colletotrichum gloeosporioides and an LAMP primer used in the method; and the method can be used for specifically detecting strawberry colletotrichum gloeosporioides with high resistance toQoI germicides. The method disclosed by the invention has the characteristics of simplicity, quickness, low cost, high sensitivity and the like, can greatly improve the detection efficiency, and has important practical significance for seedling stage detection and drug resistance epidemic early warning of strawberry anthracnose.
Owner:ZHEJIANG FORESTRY UNIVERSITY
Primer and detection method for real-time fluorescent quantitative PCR detection of colletotrichum gloeosporioides of hevea brasiliensis
PendingCN111534621AQuantitatively accurateHigh sensitivityMicrobiological testing/measurementMicroorganism based processesDiseaseMicrobiology
The invention discloses a primer and a detection method for real-time fluorescent quantitative PCR detection of colletotrichum gloeosporioides of a hevea brasiliensis. A specific primer is designed according to a ribosome ITS sequence of colletotrichum gloeosporioides of the hevea brasiliensis, quantitative analysis is performed on the colletotrichum gloeosporioides through real-time fluorescent quantitative PCR, and the change rule of DNA of the colletotrichum gloeosporioides in tissue after inoculation of leaves of the hevea brasiliensis along with time is determined. The hevea brasiliensiscolletotrichum gloeosporioides quantitative detection technology provided by the invention is high in specificity, high in sensitivity, high in repeatability, accurate in quantification, high in speedand capable of achieving a full-closed reaction, colletotrichum gloeosporioides is identified and can be accurately quantified, the thallus content of hevea brasiliensis leaves at different time points after thallus infection is obtained, and therefore, early warning and preventive measures are carried out when the disease does not appear, the loss caused by the disease is reduced to the minimum,and the method is of great significance to early diagnosis, disease prediction and comprehensive prevention and treatment of the disease.
Owner:HAINAN UNIVERSITY
A methylotrophic bacillus strain for biocontrol and application thereof
ActiveCN109182217ABroad antibacterial spectrumEnhanced inhibitory effectFruit and vegetables preservationBacteriaMolecular identificationStaphylococcus aureus
A methylotrophic bacillus strain (Bacillus methylotrophicus) for biocontrol and application thereof are disclosed. The bacterium Bacillus methylotrophicus BCN2 is deposited in the China General Microbiological Culture Collection Center under the accession number CGMCC No: 16443. The invention comprises isolation and culture of the strain and molecular identification of 16S rDNA; effective inhibiting effects of Methylotrophic Bacillus volatiles against common spoilage fungi (Fusarium oxysporum, Staphylococcus aureus, Trichoderma viride, Colletotrichum gloeosporioides, Penicillium expansum) ontwo-zone plate and loquat fruits and the antiseptic application of the volatile matter in loquat storage. The volatile antimicrobial substances produced by Bacillus methylotrophicus (BCN2) could not contact fruit directly, It can be used for preparing biocontrol fresh-keeping agent which can inhibit spoilage fungi such as Fusarium oxysporum, Staphylococcus aureus, Trichoderma viride, Colletotrichum gloeosporioides, Penicillium expansum and so on, and has wide bacteriostasis spectrum.
Owner:ZHEJIANG UNIV
Yangbi walnut germin-like protein gene JsGLP1 and application thereof
ActiveCN104878019AIncrease resistanceReduce usageFermentationGenetic engineeringBiotechnologyNicotiana tabacum
The invention discloses a Yangbi walnut germin-like protein gene JsGLP1 and an application thereof. The nucleotide sequence of the JsGLP1 gene is as shown in SEQ ID NO:1, and is capable of encoding germin-like protein. Through functional genomics related technological research, the gene JsGLP1 is proved to have the function of enhancing plant fungal infection resistance; the antifungal gene disclosed by the invention is constructed on a plant expression vector and is transferred into tobacco for over expression; the transgenic tobacco plant is strong in the in-vitro antifungal activity; and JsGLP1 over-expression transgenic tobacco has a significant inhibitory effect on the growth of sclerotinia sclerotiorum, beaded gibberella, colletotrichum gloeosporioides and fusarium oxysporum.
Owner:KUNMING UNIV OF SCI & TECH
Application of Noni endophytic bacillus siamensis
ActiveCN113308392AStabilized broad-spectrum antagonistic activityAvoid infectionBiocideBacteriaBiotechnologyFragaria
The invention provides noni endophytic bacillus siamensis, which is named as bacillus siamensis QN2MO-1, is stored in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC M 2021305. The strain has stable broad-spectrum antagonistic activity; and strong antibacterial effect on tomato fusarium wilt, banana fusarium wilt, cucumber fusarium wilt, pepper colletotrichum gloeosporioides, strawberry colletotrichum gloeosporioides, banana colletotrichum gloeosporioides, cabbage colletotrichum gloeosporioides, corn curvularia leaf spot, mango leaf blight, wheat scab, cryphonectria parasitica, mango pestalotiopsis leaf blight, rice blast, and colletotrichum gloeosporioides; its fermentation broth can effectively inhibit the infection of pathogen filaments on in-vitro leaves, fruits and the like, increases the safe sources of the effective components of the biocontrol agent, expands a new field for the prevention and treatment of tomato fusarium wilt and other plant diseases, and has good development and application prospects.
Owner:INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI +1
Microorganism derived from Chinese chestnuts and application thereof as well as microbial agent preparation method
The invention relates to the field of a microorganism, and particularly relates to a microorganism derived from Chinese chestnuts and an application thereof as well as a microbial agent preparation method. The microorganism derived from Chinese chestnuts is collected at the China Center for Type Culture Collection (CCTCC) on November 12, 2013 with the CCTCC No. M2013568, and the collection name of the microorganism is bacillus amyloliquefaciens subspecies CMN1308. The microorganism is an endophyte of Chinese chestnuts and has good effects in the terms of preventing and treating Chinese chestnut seed rot caused by at least one of cryponectria parasitica, fusarium solani, stachybotrys atra, penicillium expansum and colletotrichum, serving as a feed additive to promote the growth of animals and improve the immune ability and the like. Compared with the prior art using chemical pesticides for preventing and treating the seed rot, the microorganism does not cause the harm to human health and environmental pollution, and has important environmental protection significance.
Owner:HUANGGANG NORMAL UNIV
Antifungal streptomyces hygroscopicus and application thereof
ActiveCN113957003AStable broad-spectrum antibacterial activityAffect normal growth and developmentPlant growth regulatorsBiocideBiotechnologySporeling
The invention provides streptomyces hygroscopicus, which is named streptomyces hygroscopicus PA-4 and is preserved in Guangdong Microbial Culture Collection Center on May 24, 2021, and the preservation number is GDMCC No.61679. The strain has stable and broad-spectrum bacteriostatic activity and has a relatively strong bacteriostatic effect on various common crop pathogenic fungi such as fusarium oxysporum, colletotrichum, cryptoscoparia, corynespora, alternaria, fusarium and the like, a fermentation crude extract of the strain can influence normal growth and development of Foc4 mycelia, spores and the like and has a poisoning or inhibiting effect on Foc4 germs. In addition, the streptomyces hygroscopicus disclosed by the invention can also inhibit pathogenic bacteria from infecting plant root systems and promote plant growth, expands a new field for preventing and treating various plant diseases such as fusarium wilt, promoting plant growth and the like, and has a good development and application prospect.
Owner:HAIKOU EXPERIMENTAL STATION CHINESE ACAD OF TROPICAL AGRI SCI
Application of Yangbi big-bubble walnut transcription factor gene JsWRKY1
ActiveCN104878041AIncreased fungal resistanceIncrease resistanceGenetic engineeringFermentationBiotechnologyNicotiana tabacum
The invention discloses application of a Yangbi big-bubble walnut transcription factor gene JsWRKY1. The JsWRKY1 gene is proved to have a function of enhancing the fungal disease resistance of a plant through technical research related to functional genomics. The antifungal gene JsWRKY1 is established to a plant expression vector and transferred into a tobacco for overexpression, and a transgenic tobacco plant has very high in-vitro antifungal activity, and a transgenic tobacco subjected to JsWRKY1 overexpression achieves obvious inhibiting effect on the growth of botryosphaeria dothidea, moniliform gibberella, colletotrichum gloeosporioides and fusarium oxysporum.
Owner:KUNMING UNIV OF SCI & TECH
Method for detecting bacillus subtilis Y13 volatile bacteriostatic substances based on HS-SPME and GC-MS
InactiveCN110988184AGood antibacterial effectAvoid it happening againComponent separationWithdrawing sample devicesBiotechnologyChemical compound
The invention belongs to the field of testing, and particularly discloses a method for detecting bacillus subtilis Y13 volatile bacteriostatic substances based on HS-SPME technology and GC-MS. According to the method, volatile substances of bacillus subtilis Y13 are collected by utilizing the HS-SPME technology, and GC-MS is used for detecting the components and amounts of the volatile substances.The method can comprehensively and accurately determine the components and amounts of the bacillus subtilis Y13 volatile substances, also can be completed in a normal-temperature range, and avoids the generation of a new compound in an analysis process. The working conditions of the HS-SPME technology provided by the invention can effectively enrich each component of the Y13 volatile substances;GC-MS detection adopts sectional heating, volatile substance components can be effectively separated, and the volatile substance components can be rapidly and accurately identified through corresponding mass spectrum parameters; in addition, colletotrichum fructicola is used as an indicator bacterium to identify the effective components of the bacillus subtilis Y13 volatile substances with strongbacteriostatic effect.
Owner:HAINAN KEDA FORESTRY CO LTD +1
Application of colletotrichum gloeosporioides fatty acid hydroxylase CsSCS7 and method for constructing gene knockout vector and gene knockout mutant
ActiveCN112280757APromote degradationFungiMicroorganism based processesFatty acid hydroxylaseGenetics
The invention provides an application of colletotrichum gloeosporioides fatty acid hydroxylase CsSCS7 and a method for constructing a gene knockout vector and a gene knockout mutant, and particularlyrelates to the application of the colletotrichum gloeosporioides fatty acid hydroxylase CsSCS7 in degrading bactericides, the CsSCS7 gene knockout mutant is obtained by constructing a CsSCS7 gene knockout vector, through functional tests, it is proved that the hydroxylase is extremely important for growth of colletotrichum gloeosporioides hyphae, participates in regulation and control of degradation of fungi to fludioxonil, can be applied to degradation of pyrrole sterilizing agents such as fludioxonil and has a good application prospect.
Owner:HAINAN UNIVERSITY
Bacillus velezensis and application thereof in preventing and treating various vegetable diseases
The invention discloses bacillus velezensis and an application thereof in preventing and treating various vegetable diseases. The bacillus velezensis with strain number of ZF438 is preserved in the China General Microbiological Culture Collection Center with registration number of CGMCC No.22291. Experiments prove that the bacillus velezensis ZF438 has an inhibition effect on various pathogenic fungi and bacteria, and has a relatively good inhibition effect on the pathogenic fungi such as rhizoctonia solani, colletotrichum capsici and stemphylium lycopersici. Through in-vitro and potting control effect determination on anthracnose of capsicum fruits, the control effect of the bacillus velezensis ZF438 on the colletotrichum capsici is obviously higher than that of a control group. Meanwhile, the control effect on cucumber soft rot disease and the potting control effect on tomato bacterial wilt are both higher than a control group. In actual production, the bacillus velezensis ZF438 can be widely applied to prevention and treatment of various vegetable diseases.
Owner:INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
Trichoderma fermentation medium for antagonizing tea colletotrichum bacteria and fermentation method
PendingCN112111413AHigh substance contentImprove filtration efficiencyBiocideFungiBiotechnologyDipotassium hydrogen phosphate
The invention discloses a trichoderma fermentation medium for antagonizing tea colletotrichum bacteria and a fermentation method, and relates to the technical field of microbial fermentation. According to key points of the technical scheme, the diameter of a tea colletotrichum bacterial colony treated by trichoderma fermentation liquor prepared by the fermentation medium and the fermentation method designed by the invention is 2.98-6.50 cm; the diameter of the tea colletotrichum bacterial colony is generally lower than the diameter 7.0 cm of a tea colletotrichum bacterial colony treated by trichoderma fermentation liquor prepared by an existing trichoderma culture medium, wherein the optimal fermentation medium is prepared from 200 g / L of potatoes, 18.34 g / L of glucose, 1.88 g / L of yeast extract, 0.86 g / L of dipotassium hydrogen phosphate and 0.63 g / L of ferrous sulfate. Under the condition, the diameter of the tea colletotrichum bacterial colony treated by the trichoderma fermentationliquor is 3.01 cm and is reduced by 13% compared with the diameter of the bacterial colony before optimization. A liquid fermentation medium is designed for trichoderma with an effect of antagonizingthe tea colletotrichum bacteria, so that the content of substances for inhibiting the growth of the tea colletotrichum bacteria in the trichoderma fermentation liquor is increased, the cost is savedto a certain extent, and the fermentation efficiency is improved.
Owner:贵州省生物技术研究所
Method of controlling anthracnose on tropical fruit plants
A method of controlling anthracnose caused by Colletotrichum on tropical fruits, tropical fruit plants or cultivars or plant parts or locus thereof is described. The method comprises the steps of contacting the tropical fruit plant or cultivar in need of treatment with an agrochemical composition comprising an effective amount of a guanidine or a salt and / or solvate thereof. The contacting step may be repeated one or more times at a fixed interval. The guanidine may be dodine, iminoctadine trialbesilate, iminoctadine triacetate, guazatine, salts and / or solvates of any of the foregoing and combinations of one or more of the foregoing.
Owner:ARYSTA LIFESCIENCE INC
PCR primer and quantitative detection method for rapidly detecting strawberry colletotrichum gloeosporioides
InactiveCN111893208ARapid implementation of identification workHigh amplification efficiencyMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyFragaria
The invention relates to a PCR primer and quantitative detection method for rapidly detecting strawberry colletotrichum gloeosporioides. The PCR primer is designed according to a cutinase gene consensus sequence of colletotrichum gloeosporioides physiological races and comprises two primers Cfcut1 and Cfcut2 and an amplification product 115bp; by utilizing the PCR primer and the quantitative detection method, fungal genome DNA or field sample DNA can be subjected to PCR detection without separation and culture of pathogenic bacteria, the amplification efficiency is high, the specificity is good, the amplification efficiency of the primer is 99.47%, and colletotrichum gloeosporioides compound species causing strawberry anthracnose can be rapidly detected and identified; and through real-time fluorescent quantitative PCR, absolute quantitative detection can be carried out on the biomass of the colletotrichum gloeosporioides in a sample, the occurrence period and the prevalence intensityof the anthracnose are predicted timely and accurately, the anthracnose is prevented and treated and even avoided, negative influences caused by use of pesticide can be reduced, and a basis is provided for comprehensive prevention and treatment of the anthracnose.
Owner:SHANGHAI ACAD OF AGRI SCI
Biocontrol Bacillus thuringiensis strain and application thereof
ActiveCN109988726AAchieve freshnessExtended storage timeFruit and vegetables preservationBacteriaDiseaseAureobasidium sp.
The invention discloses a biocontrol Bacillus thuringiensis strain and an application thereof. The collection number of Bacillus thuringiensis BCN10 is CGMCC No: 16675. Fermentation liquor of the Bacillus thuringiensis effectively inhibits common loquat surface putrefying fungi (fusarium oxysporum, botryosphaeria parva, trichoderma aureoviride, colletotrichum gloesporioides and penicillium expansum) on a bi-zone flat plate and a loquat fruit. Volatile substances generated by the fermentation liquor are applied to corrosion resistance in loquat storage, natural putrefaction disease time of theloquat fruit can be delayed, and incidence rate is decreased. Volatile antibacterial substances generated by the Bacillus thuringiensis BCN10 can avoid direct contact with the fruit, the fruit is preserved at normal temperature, food storage time is prolonged, and the strain can be used for preparing a biocontrol preservative which is used for inhibiting the putrefying fungi such as the fusarium oxysporum, the botryosphaeria parva, the trichoderma aureoviride, the colletotrichum gloesporioides and the penicillium expansum and has a wide antibacterial spectrum.
Owner:ZHEJIANG UNIV
Bacillussubtilis used for inhibiting formation of Colletotrichum capsici appressoria and antibacterial peptides thereof
InactiveCN103589657AInhibition formationPrevent diseaseBacteriaMicroorganism based processesBiotechnologyBacilli
The invention relates to a Bacillussubtilis C-D6 strain used for inhibiting formation of Colletotrichum capsici appressoria and applications. The strain is collected in China General Microbiological Culture Collection Center (CGMCC). The number is CGMCCNo. 5345. The invention also provides a suitable culture method for the strain for generation of active substances for inhibiting formation of appressoria and a separation and purification method for antibacterial proteins. By utilization of the above method, the culture solution of the C-D6 strain can strongly inhibit formation of Colletotrichum capsici 501 appressoria. The separated antibacterial proteins from the culture solution and with a low concentration can completely inhibit formation of the strain appressoria. The C-D6 strain and the produced antibacterial proteins have specific inhibition effects, can effectively interdict invasion of pathogenic bacteria, and can be used for biological prevention and control of crop anthracnose caused by Colletotrichum capsici.
Owner:SHENZHEN POLYTECHNIC
Application of Bacillus velezensis YM-11-C for preventing and curing colletotrichum gloeosporioides Penz.
ActiveCN112029691ANo pollution in the processGood effectBiocideFruit and vegetables preservationBiotechnologyHypha
The invention relates to application of Bacillus velezensis YM-11-C for preventing and curing colletotrichum gloeosporioides Penz.. One strain of Bacillus velezensis YM-11-C is screened from the peelof Mangifera indica L. and has a collection number of CGMCC No. 20599. Fermentation broth for the strain of Bacillus velezensis YM-11-C has an inhibition function for Colletotrichum fructicola hyphae,and volatile gases generated by the fermentation broth can inhibit growth of the Colletotrichum fructicola hyphae, and have a good preventing and curing effect on the indoor preventing and curing ofcolletotrichum gloeosporioides Penz. After the Mangifera indica L. is harvested.
Owner:INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
Rubber tree colletotrichum drug-resistant strain HcgHNQZ1736 and application thereof in drug resistance research
The invention belongs to the technical field of plant genetic engineering, and particularly relates to karst colletotrichum and application thereof in the field of rubber anthracnose research. The karst colletotrichum is found on rubber trees in Hainan Province for the first time; and a strain obtained through separation is C.karstii. Biological characteristic determination finds that the optimumgrowth temperature of the strain is 28 DEG C; the lethal temperature is 35 DEG C; the optimum growth pH is 6; light and dark alternation is beneficial to colony growth; and pectin and peptone are respectively an optimal carbon source and an optimal nitrogen source. Pathogenicity evaluation finds that the strain has relatively strong pathogenicity on four main recommended varieties including PR107,RRIM600, hot research 7-33-97 and Dafeng 95; a drug resistance evaluation result shows that the strain HcgHNQZ1736 has no drug resistance to a sterol demethylation inhibitor (DMIs) bactericide prochloraz-manganese chloride complex, and has high drug resistance to a benzimidazole bactericide carbendazim.
Owner:ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
Cunninghamia lanceolata Epicoccum dendrobii fermentation filtrate, extract, preparation method and application thereof
ActiveCN111808888ADecreased appressoria formation rateImprove the effect of prevention and controlBiocideFungiBiotechnologySporeling
The invention discloses a cunninghamia lanceolata Epicoccum dendrobii fermentation filtrate, an extract, a preparation method and an application thereof. The fermentation filtrate of the Epicoccum dendrobii SMEL disclosed by the invention can be used for remarkably inhibiting the colletotrichum gloeosporioides spore germination of the cunninghamia lanceolata and inhibiting the appressorium development of the colletotrichum gloeosporioides of the cunninghamia lanceolata; the ethyl acetate extract of the Epicoccum dendrobii SMEL fermentation filtrate can obviously inhibit the mycelial growth ofthe colletotrichum gloeosporioides of the cunninghamia lanceolata, and obviously inhibit the spore germination and appressorium development of the colletotrichum gloeosporioides of the cunninghamia lanceolata; and an inoculation test shows that the ethyl acetate extract obviously inhibits the pathogenicity of colletotrichum gloeosporioides of the cunninghamia lanceolata.
Owner:NANJING FORESTRY UNIV
Rapid molecular detection method for pathogenic bacterium colletotrichum siamese of colletotrichum leaf disease of rubber trees, and application of primer
PendingCN111321242AEfficient identificationShorten identification timeMicrobiological testing/measurementMicroorganism based processesBiotechnologyMicrobiology
The invention discloses a special primer for identifying a pathogenic bacterium colletotrichum siamese of a colletotrichum leaf disease of rubber trees, a rapid molecular detection method of the colletotrichum siamese, and an application of the primer. The special primer for identifying the colletotrichum siamese is a primer pair consisting of a sequence 1 and a sequence 2. According to the rapidmolecular detection method, a PCR reaction is carried out on DNA contained in a sample, and when a PCR product presents a 416bp specific product, the sample is the pathogenic bacterium colletotrichumsiamese. The method has simple operation and high specificity, and the lowest detection content of fungus DNA is 10pg.
Owner:ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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