97 results about "Colletotrichum sp." patented technology

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

The invention discloses bacillus methylotrophicus F7 and an application thereof. The invention aims at providing a strain which has a plurality of beneficial properties, namely producing siderophore, cellulase and protease, producing auxin, producing ammonia and the like; the strain not only can be used for effectively inhibiting various pathogenic bacteria such as ralstonia solanacearum, fusarium oxysporum f.sp.cubense, colletotrichum gloeosporioides, fusarium oxysporum f.sp cucumerinum but also can promote plant growth. In accordance with the technical scheme, the bacillus methylotrophicus F7 is preserved in China General Microbiological Culture Collection Center (CGMCC) on February 1, 2016, with preservation number of CGMCC No.12124; in addition, five surfactin homologs, six fengycin homologs and three iturin homologs, which have an antibacterial effect, are extracted from fermentation liquid of the bacillus methylotrophicus F7; and the bacillus methylotrophicus F7 belongs to the field of agricultural biotechnology.

The invention discloses application of bacillus amyloliquefaciens in preventing and controlling fungal disease of plants. The application has the advantages that found by study, the bacillus amyloliquefaciens, especially bacillus amyloliquefaciens BSY3 separated and screened from African marigold by an inventor, has obvious antagonistic function on phellinus noxius, pyricularia cannaecola hashioka, sclerotinia sclerotiorum, colletotrichum sp., pestalotiopsissp, fusarium oxysporum f.sp.cubense and the like, and has obvious biological control function and potential application value on preventing and controlling of the fungi diseases due to pathogenic bacteria; the bacillus amyloliquefaciens comes from endophytic bacteria in plants, so that compared with the traditional chemical agent preventing and control method, the safer biological control effect on the fungi diseases is realized; because the bacillus amyloliquefaciens stays in the plant for a long time, the survival relationship with harmonious coexistence and stable development is established with the plant; compared with the chemical agent, the biocontrol function can be more effectively and stably realized.

The invention mainly provides a thiosemicarbazone compound which has an inhibition effect on fusarium oxysporum, fusarium moniliforme, fusarium solani and colletotrichum and a preparation method thereof. The chemical structural formula of the compound is shown in the specification. According to the invention, a novel way for preventing and controlling plant diseases caused by fusarium oxysporum, fusarium moniliforme, fusarium solani and colletotrichum is provided; and the preparation method is simple and convenient and has strong operability.

The invention relates to the technical field of microbes and especially relates to huperzia serrata endophytic fungi and its use in production of huperzine a. The huperzia serrata endophytic fungi are named as Colletotrichum sp. SY-1, are preserved in the China center for type culture collection (CCTCC) on August 19, 2011, and have the preservation number CCTCC M2011293. Through fermentation, the huperzia serrata endophytic fungi can produce a huperzine a compound. Through utilization of characteristics of microbe-based huperzine a production and the modern fermentation breeding technology, huperzine a industrial production is realized; bottleneck problems of huperzine a exploitation are solved; and the endangered huperzine a natural resource is protected.

The invention belongs to the field of biological pesticides, and discloses a sugarcane endophytic bacillus amyloliquefaciens RGB15 strain and an application thereof. The bacillus amyloliquefaciens CGB15 strain is preserved in the Guangdong Province Microbial Culture Collection Center on October 17, 2019, and the preservation number is GDMCC No: 60742, and the preservation address is the 5th floorof No.59 building of No.100 courtyard of the Xianlie Middle Road of Guangzhou city. The bacillus amyloliquefaciens RGB15 strain has a strong inhibition effect on ustilago scitaminea, pyricularia grisea, peronophythora litchii, colletotrichum litchii and banana fusarium oxysporum, and has an excellent biological control effect. A microbial preparation prepared from the strain is simple in preparation and use method, has broad-spectrum disease resistance, has little influence on the ecological environment, does not influence the crop quality, and has an excellent application prospect.

The invention discloses a primer and a detection method for real-time fluorescent quantitative PCR detection of colletotrichum gloeosporioides of a hevea brasiliensis. A specific primer is designed according to a ribosome ITS sequence of colletotrichum gloeosporioides of the hevea brasiliensis, quantitative analysis is performed on the colletotrichum gloeosporioides through real-time fluorescent quantitative PCR, and the change rule of DNA of the colletotrichum gloeosporioides in tissue after inoculation of leaves of the hevea brasiliensis along with time is determined. The hevea brasiliensiscolletotrichum gloeosporioides quantitative detection technology provided by the invention is high in specificity, high in sensitivity, high in repeatability, accurate in quantification, high in speedand capable of achieving a full-closed reaction, colletotrichum gloeosporioides is identified and can be accurately quantified, the thallus content of hevea brasiliensis leaves at different time points after thallus infection is obtained, and therefore, early warning and preventive measures are carried out when the disease does not appear, the loss caused by the disease is reduced to the minimum,and the method is of great significance to early diagnosis, disease prediction and comprehensive prevention and treatment of the disease.

A methylotrophic bacillus strain (Bacillus methylotrophicus) for biocontrol and application thereof are disclosed. The bacterium Bacillus methylotrophicus BCN2 is deposited in the China General Microbiological Culture Collection Center under the accession number CGMCC No: 16443. The invention comprises isolation and culture of the strain and molecular identification of 16S rDNA; effective inhibiting effects of Methylotrophic Bacillus volatiles against common spoilage fungi (Fusarium oxysporum, Staphylococcus aureus, Trichoderma viride, Colletotrichum gloeosporioides, Penicillium expansum) ontwo-zone plate and loquat fruits and the antiseptic application of the volatile matter in loquat storage. The volatile antimicrobial substances produced by Bacillus methylotrophicus (BCN2) could not contact fruit directly, It can be used for preparing biocontrol fresh-keeping agent which can inhibit spoilage fungi such as Fusarium oxysporum, Staphylococcus aureus, Trichoderma viride, Colletotrichum gloeosporioides, Penicillium expansum and so on, and has wide bacteriostasis spectrum.

The invention discloses a Yangbi walnut germin-like protein gene JsGLP1 and an application thereof. The nucleotide sequence of the JsGLP1 gene is as shown in SEQ ID NO:1, and is capable of encoding germin-like protein. Through functional genomics related technological research, the gene JsGLP1 is proved to have the function of enhancing plant fungal infection resistance; the antifungal gene disclosed by the invention is constructed on a plant expression vector and is transferred into tobacco for over expression; the transgenic tobacco plant is strong in the in-vitro antifungal activity; and JsGLP1 over-expression transgenic tobacco has a significant inhibitory effect on the growth of sclerotinia sclerotiorum, beaded gibberella, colletotrichum gloeosporioides and fusarium oxysporum.

The invention provides noni endophytic bacillus siamensis, which is named as bacillus siamensis QN2MO-1, is stored in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC M 2021305. The strain has stable broad-spectrum antagonistic activity; and strong antibacterial effect on tomato fusarium wilt, banana fusarium wilt, cucumber fusarium wilt, pepper colletotrichum gloeosporioides, strawberry colletotrichum gloeosporioides, banana colletotrichum gloeosporioides, cabbage colletotrichum gloeosporioides, corn curvularia leaf spot, mango leaf blight, wheat scab, cryphonectria parasitica, mango pestalotiopsis leaf blight, rice blast, and colletotrichum gloeosporioides; its fermentation broth can effectively inhibit the infection of pathogen filaments on in-vitro leaves, fruits and the like, increases the safe sources of the effective components of the biocontrol agent, expands a new field for the prevention and treatment of tomato fusarium wilt and other plant diseases, and has good development and application prospects.

The invention relates to the field of a microorganism, and particularly relates to a microorganism derived from Chinese chestnuts and an application thereof as well as a microbial agent preparation method. The microorganism derived from Chinese chestnuts is collected at the China Center for Type Culture Collection (CCTCC) on November 12, 2013 with the CCTCC No. M2013568, and the collection name of the microorganism is bacillus amyloliquefaciens subspecies CMN1308. The microorganism is an endophyte of Chinese chestnuts and has good effects in the terms of preventing and treating Chinese chestnut seed rot caused by at least one of cryponectria parasitica, fusarium solani, stachybotrys atra, penicillium expansum and colletotrichum, serving as a feed additive to promote the growth of animals and improve the immune ability and the like. Compared with the prior art using chemical pesticides for preventing and treating the seed rot, the microorganism does not cause the harm to human health and environmental pollution, and has important environmental protection significance.

The invention provides streptomyces hygroscopicus, which is named streptomyces hygroscopicus PA-4 and is preserved in Guangdong Microbial Culture Collection Center on May 24, 2021, and the preservation number is GDMCC No.61679. The strain has stable and broad-spectrum bacteriostatic activity and has a relatively strong bacteriostatic effect on various common crop pathogenic fungi such as fusarium oxysporum, colletotrichum, cryptoscoparia, corynespora, alternaria, fusarium and the like, a fermentation crude extract of the strain can influence normal growth and development of Foc4 mycelia, spores and the like and has a poisoning or inhibiting effect on Foc4 germs. In addition, the streptomyces hygroscopicus disclosed by the invention can also inhibit pathogenic bacteria from infecting plant root systems and promote plant growth, expands a new field for preventing and treating various plant diseases such as fusarium wilt, promoting plant growth and the like, and has a good development and application prospect.

The invention discloses application of a Yangbi big-bubble walnut transcription factor gene JsWRKY1. The JsWRKY1 gene is proved to have a function of enhancing the fungal disease resistance of a plant through technical research related to functional genomics. The antifungal gene JsWRKY1 is established to a plant expression vector and transferred into a tobacco for overexpression, and a transgenic tobacco plant has very high in-vitro antifungal activity, and a transgenic tobacco subjected to JsWRKY1 overexpression achieves obvious inhibiting effect on the growth of botryosphaeria dothidea, moniliform gibberella, colletotrichum gloeosporioides and fusarium oxysporum.

The invention discloses a trichoderma fermentation medium for antagonizing tea colletotrichum bacteria and a fermentation method, and relates to the technical field of microbial fermentation. According to key points of the technical scheme, the diameter of a tea colletotrichum bacterial colony treated by trichoderma fermentation liquor prepared by the fermentation medium and the fermentation method designed by the invention is 2.98-6.50 cm; the diameter of the tea colletotrichum bacterial colony is generally lower than the diameter 7.0 cm of a tea colletotrichum bacterial colony treated by trichoderma fermentation liquor prepared by an existing trichoderma culture medium, wherein the optimal fermentation medium is prepared from 200 g/L of potatoes, 18.34 g/L of glucose, 1.88 g/L of yeast extract, 0.86 g/L of dipotassium hydrogen phosphate and 0.63 g/L of ferrous sulfate. Under the condition, the diameter of the tea colletotrichum bacterial colony treated by the trichoderma fermentationliquor is 3.01 cm and is reduced by 13% compared with the diameter of the bacterial colony before optimization. A liquid fermentation medium is designed for trichoderma with an effect of antagonizingthe tea colletotrichum bacteria, so that the content of substances for inhibiting the growth of the tea colletotrichum bacteria in the trichoderma fermentation liquor is increased, the cost is savedto a certain extent, and the fermentation efficiency is improved.

The invention relates to application of Bacillus velezensis YM-11-C for preventing and curing colletotrichum gloeosporioides Penz.. One strain of Bacillus velezensis YM-11-C is screened from the peelof Mangifera indica L. and has a collection number of CGMCC No. 20599. Fermentation broth for the strain of Bacillus velezensis YM-11-C has an inhibition function for Colletotrichum fructicola hyphae,and volatile gases generated by the fermentation broth can inhibit growth of the Colletotrichum fructicola hyphae, and have a good preventing and curing effect on the indoor preventing and curing ofcolletotrichum gloeosporioides Penz. After the Mangifera indica L. is harvested.

The invention discloses a special primer for identifying a pathogenic bacterium colletotrichum siamese of a colletotrichum leaf disease of rubber trees, a rapid molecular detection method of the colletotrichum siamese, and an application of the primer. The special primer for identifying the colletotrichum siamese is a primer pair consisting of a sequence 1 and a sequence 2. According to the rapidmolecular detection method, a PCR reaction is carried out on DNA contained in a sample, and when a PCR product presents a 416bp specific product, the sample is the pathogenic bacterium colletotrichumsiamese. The method has simple operation and high specificity, and the lowest detection content of fungus DNA is 10pg.