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Application of Bacillus velezensis YM-11-C for preventing and curing colletotrichum gloeosporioides Penz.

A technology for bacillus and anthracnose, applied in the application, bacteria, fungicides and other directions, can solve the problems of high cost and drug resistance of pathogenic bacteria, and achieve the effect of good effect, reduction of secondary pollution, good effect and prospect

Active Publication Date: 2020-12-04
INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the aspect of postharvest pathogen suppression and preservation of mangoes, chemical control is mostly used, but the cost of chemical control is high, and long-term use of these chemical agents will cause pathogenic bacteria to develop drug resistance

Method used

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  • Application of Bacillus velezensis YM-11-C for preventing and curing colletotrichum gloeosporioides Penz.
  • Application of Bacillus velezensis YM-11-C for preventing and curing colletotrichum gloeosporioides Penz.
  • Application of Bacillus velezensis YM-11-C for preventing and curing colletotrichum gloeosporioides Penz.

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The isolation, screening and identification of embodiment 1 Veles bacillus YM-11-C

[0039] 1.1 Isolation of bacteria

[0040] Take a healthy mango epidermis, and in an ultra-clean workbench, first sterilize it with 75% alcohol for 10 seconds, then sterilize it with 2% NaClO for 1 minute, and finally rinse it with sterile water for 3 times, cut it into tissue pieces of about 3×3mm, After drying. Place 3-4 tissue pieces on the PDA medium and culture them in a 25°C incubator for 3-5 days. When colonies grow out of the tissue blocks, bacteria with different colony forms are picked to be purified and cultured on PDA medium and labeled.

[0041] 1.2 Primary screening of bacteria

[0042] Preliminary screening: Using the plate confrontation method, use a strain of Mango anthracis as an indicator bacterium to screen the bacterial strains isolated and purified in 1.1 on a PDA plate, and save the antagonistic strains for future use.

[0043] Re-screening: Screen the bacteria...

Embodiment 2

[0049] The influence of embodiment 2 Veles bacillus YM-11-C fermented liquid on mango anthracnose mycelium

[0050] The mango anthracnose fungus Colletotrichum fructicola was activated on a PDA plate and cultured at a constant temperature of 25°C for 5-7d. Use a sterilized puncher with a diameter of 5 mm to punch holes in the cultured anthrax bacteria, and place them in the center of a new PDA plate. With the center of the petri dish as the origin, place sterilized 5mm circular filter paper pieces at a distance of 2.5cm from the center, pipette 10 μL of bacterial solution with a pipette gun, and drop it on the filter paper pieces. Three replicates were set up for each treatment, and the treatment with water added was used as a control, and the culture was incubated at a constant temperature of 27°C. When the control colony is full of the plate, the diameter of the pathogenic bacteria colony is measured by the cross method, and the average value and the bacteriostatic rate are...

Embodiment 3

[0051] Embodiment 3 The impact of the volatile gas of Bacillus Velez YM-11-C on the hyphae of the mango anthracis fungus

[0052] Using the buckle method, put a sterile filter paper sheet with a diameter of 5mm in the center of the NA plate, and add 50μL of YM-11-C fermentation broth dropwise; use a puncher with a diameter of 5mm to cultivate the mango anthracnose bacteria for 5-7 days (Colletotrichum fructicola) bacterium cake was taken from the edge of the colony, and the bacterium cake was inoculated in the center of the PDA plate. The PDA plate inoculated with the mango anthracnose bacteria is buckled with the NA plate inoculated with the bacterial fermentation broth, and sealed. The NA plate not inoculated with bacterial fermentation broth was used as the control, and three replicates were set up for each treatment, and cultured at a constant temperature of 27°C. When the control colony is full of the plate, the diameter of the pathogenic bacteria colony is measured by t...

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Abstract

The invention relates to application of Bacillus velezensis YM-11-C for preventing and curing colletotrichum gloeosporioides Penz.. One strain of Bacillus velezensis YM-11-C is screened from the peelof Mangifera indica L. and has a collection number of CGMCC No. 20599. Fermentation broth for the strain of Bacillus velezensis YM-11-C has an inhibition function for Colletotrichum fructicola hyphae,and volatile gases generated by the fermentation broth can inhibit growth of the Colletotrichum fructicola hyphae, and have a good preventing and curing effect on the indoor preventing and curing ofcolletotrichum gloeosporioides Penz. After the Mangifera indica L. is harvested.

Description

technical field [0001] The invention relates to the prevention and treatment of plant diseases by antagonistic microorganisms, in particular to the application of Bacillus Velez YM-11-C in the prevention and treatment of mango anthracnose. Background technique [0002] Mango is an important economic crop in subtropical and tropical regions of my country. Because of its rich aroma, juicy and tender meat, sweet and sour taste and high nutritional value, it is deeply loved by people and is known as the "king of tropical fruits". The distribution of mango planting is wide, and the yield is high. Its yield ranks among the top in the world among fruits, and it is widely distributed in tropical and subtropical regions. In China, mangoes are mainly grown in Hainan, Yunnan, Sichuan, Guangdong, Guangxi, Fujian, Guizhou and other provinces. [0003] Mango production has brought great economic benefits to my country's fruit industry, but mango diseases have also caused serious threats ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/22A01P3/00A23B7/155A01G7/06A01G13/00A01G17/00C12R1/07
CPCC12N1/20A01N63/22A23B7/155A01G7/06A01G13/00A01G17/005C12R2001/07C12N1/205
Inventor 李其利杨芝霓莫贱友郭堂勋唐利华黄穗萍韦继光
Owner INST OF PLANT PROTECTION GUANGXI ACADEMY OF AGRI SCI
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