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Huperzia serrata endophytic fungi and its use in production of huperzine a

A technology of endophytic fungus, Huperzine serrata, applied in the field of microorganisms, can solve the problems of unreachable, degraded strains, and the inability to realize large-scale production of Huperzine A, etc., and achieve the effect of solving the bottleneck problem

Active Publication Date: 2013-05-15
吴水生
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Problems solved by technology

All the above studies have shown that Huperzine A can be fermented by the endophytic fungus of Huperzia serrata, but the huperzine A-producing strains isolated so far are only at the ng level, far less than the mg level, because the yield is too low , and there are problems such as strain degradation, so that large-scale production of huperzine A cannot be realized, making the shortage of huperzine A still unsolved

Method used

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  • Huperzia serrata endophytic fungi and its use in production of huperzine a

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Embodiment 1

[0023] a. Please refer to figure 1 , is the microscopic morphological characteristic figure of the bacterial strain of the present invention. The Huperzia serrata endophytic fungus bacterial strain producing huperzine A of the present invention is taken, and under aseptic conditions, a small amount of mycelium is picked with a bamboo stick, and inserted into sterilized PDA Solid medium test tube, activated at 28°C for 72 hours;

[0024] b. Take the strains activated and cultivated in step a, transfer them to the sterilized PDA solid medium plate under aseptic conditions, and cultivate them at 28°C for 10 days;

[0025] c. Take the cultured mycelia and its culture medium, chop them up, put them in a stoppered Erlenmeyer flask, add 2% tartaric acid to soak for 24 hours, ultrasonically for one hour, filter, and extract the filtrate with 2-4 times the amount of chloroform for 2- 5 times, take the acidic water solution and adjust the pH value to 9-11 with ammonia water, extract 2-...

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Abstract

The invention relates to the technical field of microbes and especially relates to huperzia serrata endophytic fungi and its use in production of huperzine a. The huperzia serrata endophytic fungi are named as Colletotrichum sp. SY-1, are preserved in the China center for type culture collection (CCTCC) on August 19, 2011, and have the preservation number CCTCC M2011293. Through fermentation, the huperzia serrata endophytic fungi can produce a huperzine a compound. Through utilization of characteristics of microbe-based huperzine a production and the modern fermentation breeding technology, huperzine a industrial production is realized; bottleneck problems of huperzine a exploitation are solved; and the endangered huperzine a natural resource is protected.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an endophytic fungus of Huperzia serrata and its application for producing huperzine A. Background technique [0002] Alzheimer's disease is a serious, degenerative brain disease. Due to its high incidence rate and high disability rate, it has caused serious harm and huge burden to society and families; the treatment of Alzheimer's disease is one of the current social tasks. big problem. Huperzine A was launched in the market in 1994. It is an efficient and safe drug for the treatment of Alzheimer's disease with independent intellectual property rights in my country. Compared with similar drugs developed abroad, it has fewer adverse reactions, lower prices, and smaller doses. Advantages, has broad application prospects. At present, Huperzine A mainly comes from the whole herb of the wild plant Huperzia serrata, which grows slowly, has a small biomass, and has a narrow dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/12C12R1/645
Inventor 吴水生王明兹刘海元
Owner 吴水生
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