Rapid molecular detection method for pathogenic bacterium colletotrichum siamese of colletotrichum leaf disease of rubber trees, and application of primer
A technology for rubber tree anthracnose and pathogenic bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, and microorganism measurement/inspection, can solve the problems that there are no composite species-specific primers and PCR detection methods, etc., to achieve Rapid and efficient identification of bacterial species, shortened identification time, early diagnosis and timely prevention, reliable results
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[0020] Embodiment 1, be used for the acquisition of the Siamese anthracis primer that detects rapidly
[0021] The ApMat gene sequence of different anthrax species obtained by sequencing in our laboratory was compared with the ApMat gene sequence registered in the NCBI database, and specific primers were designed according to the difference between Siamese anthrax and other species, and the obtained primers were the invention. The primers, the primer sequence is upstream primer CSAMPF: 5'-AGTCGTGACAGCGGCTGTGT-3' (sequence 1 in the sequence listing); downstream primer CSAMPR2: 5'-ATTCCCTTCCCGCATGCTGC-3' (sequence 2 in the sequence listing).
[0022] Two, the present invention is used for the effect verification of the rapid detection primer of Siamese anthracnose
[0023] 1. Primer synthesis
[0024] Upstream primer CSAMPF: 5'-AGTCGTGACAGCGGCTGTGT-3' (sequence 1 in the sequence listing); downstream primer CSAMPR2: 5'-ATTCCCTTCCCGCATGCTGC-3' (sequence 2 in the sequence listing)...
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