The invention discloses a PBP-1A affinity beta-
lactam antibiotic electrochemical biological sensor, and a making method and an application thereof. The electrochemical biological sensor is characterized in that the surface of a
glassy carbon electrode is commonly modified by
graphene,
penicillin G and
nafion. The making method comprises the steps of bare
electrode polishing, cleaning and activating, and concretely comprises: sequentially adding
graphene and
penicillin G to the surface of the
glassy carbon electrode in a dropwise manner, well mixing, carrying out blow
drying at 37DEG C, adding
nafion in a dropwise manner, naturally air-
drying the surface, immersing the electrode in 5wt%
bovine serum albumin, placing the immersed electrode in an oven,
drying at 37DEG C for 30min, taking out the electrode, washing the electrode with a PBS solution to obtain a
glassy carbon electrode with the
surface modified by
graphene-
penicillin G-
nafion as a work electrode, placing the work electrode in a
system solution, carrying out DPV detection, and determining the concentration of beta-
lactam antibiotics in a sample to be detected according to a
standard curve. The electrochemical biological sensor has the advantages of high sensitivity, fast detection speed, low
detection limit, high efficiency and cheapness.